6-Title: Effect of seasons on in-vitro maturation and cleavage of oocyte and in-vitro development of buffalo embryo
Authors: Vijay Singh, AK Misra, Suresh Kumar and Vijay Kumar
Source: Ruminant Science (2015)-4(1):29-31.
How to cite this manuscript: Singh Vijay, Misra AK, Kumar Suresh and Kumar Vijay (2015). Effect of seasons on in-vitro maturation and cleavage of oocyte and in-vitro development of buffalo embryo. Ruminant Science 4(1):29-31.
The present study was conducted on oocytes aspirated from buffalo ovaries obtained from slaughter house. The study was conducted throughout the year dividing it into three seasons viz. summer, monsoon and winter. Oocytes were matured in tissue culture medium-199 (TCM-199) containing serum and hormones for 24 hours. Matured oocytes were co-incubated with ~1×106 sperms of Murrah buffalo bull in mSOF medium with heparin and after 22 hours of co-incubation, fertilized oocytes were stripped off of cumulus cells mass and cultured in mSOF medium for 8 days to study embryo development. Significantly (P<0.05) high maturation rate of oocytes was observed during winter (75.48%) compared to monsoon (65.53%) and summer (62.34%). Cleavage rate was also more during winter (29.94%) compared to monsoon (24.28%) and summer (21.48%), however, differences were not significant. Significantly (P<0.05) more embryos developed upto morula stage during winter (25.53%) compared to monsoon (9.52%) and summer (3.13%). Blastocyst development rate was also high during winter (10.64%) compared to monsoon (4.76%) and summer (3.13%), however, the differences were not significant.
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