Title: Genetic profiling and validation of point mutation in exon 10 of breast cancer 1 gene (BRCA1) and its relationship with clinical mastitis in Sahiwal cattle
Authors: Ankit Magotra, ID Gupta, Archana Verma, Rani Alex, Vineeth MR, Ashwani Arya, Vijay Kumar and Deepak Kumar Tiwari
Source: Ruminant Science (2016)-5(1):1-4.
Cite this reference as: Magotra Ankit, Gupta ID, Verma Archana, Alex Rani, Vineeth MR, Arya Ashwani, Kumar Vijay and Tiwari DK (2016). Genetic profiling and validation of point mutation in exon 10 of breast cancer 1 gene (BRCA1) and its relationship with clinical mastitis in Sahiwal cattle. Ruminant Science 5(1):1-4.
Breast cancer 1 (BRCA1) was cloned as one of the genes that conferred genetic predisposition to early onset breast cancer, which works in the process of DNA damage repair, cell cycle regulation, transcriptional regulation, other important pathway to inhibit tumor and make sure of the maintenance of genome stability. The objective of this study was to characterize and validate the candidate point mutation in breast cancer 1 gene, early onset gene (BRCA1) in Sahiwal cattle. The bovine BRCA1 gene was reported as one of the potential candidate gene influencing somatic cell score (SCS) and mastitis. A total of 120 Sahiwal cattle were selected to characterize targeted region i.e exon 10 of BRCA1 gene to identify polymorphism and its association with mastitis susceptibility/resistance. A PCR product of 217 bp amplifying the exon 10 of BRCA1 gene was digested with BspT I restriction enzyme to screen the reported point mutation C28300 A significantly associated with somatic cell score (SCS). All animals were found to be monomorphic with respect to reported SNP i.e (C28300A). The result indicates highly conserved sequence in Sahiwal population under study. Since present study has formulated the results based on a relatively small sample, further studies are required to explore candidate point mutation in large samples.
Das Gunjan, Lalnunpuia C, Sarma K, Behera SK, Dutta TK and Bandyopadhyay Samiran (2015). Prevalence of Staphylococcus aureus associated sub-clinical mastitis in crossbred cows in Mizoram. Ruminant Science 4(2):167-170.
Dobson H, Walker SL, Morris MJ, Routly JE and Smith RF (2008). Why is it getting more difficult to successfully artificially inseminate dairy cows? Animal 2:1104-1111.
Gupta PH, Patel NA, Rank DN and Joshi CG (2015). Genetic polymorphism of toll-like receptors 4 gene by polymerase chain reaction-restriction fragment length polymorphisms, polymerase chain reaction-single-strand conformational polymorphism to correlate with mastitic cows. Veterinary World 8(5):615-620.
Henna H, Azmat Alam K, Tufail M, Banday, Ifat A, Nida H, Asma Bashir S and Ambreen H (2013). Bovine mastitis – A disease of serious concern for dairy farmers. International Journal of Livestock Research 3:42-55.
Hogeveen H, Huijps K and Lam TJGM (2011). Economic aspects of mastitis: New developments. New Zealand Veterinary Journal 59:16-23.
Kumar Vaibhav, Patel JS, Patel BR, Mevada VK and Raval AP (2012). Therapeutic efficacy of antimicrobial drugs in clinical mastitis of cross bred cattle. Ruminant Science 1(2):177-180.
Langer Anil, AhujaAnil and Bihani DK (2014). Diagnosis of mycotic mastitis in cow. Ruminant Science 3(2):235-236.
Parmar VL, Prasad Amit, Patel JS, Dodiya PG, Javia BB and Mathpati BS (2015). Therapeutic management of clinical mastitis caused by Pseudomonas SPP with special reference to homeopathy medicine in Jafarabadi buffalo – A case study. Ruminant Science 4(2):245-246.
Raorane Abhay, Chothe Shubhadha, Dubal ZB, Barbuddhe SB, Karunakaran M, Doijad Swapnil, Pathak Ajay, Poharkar Krupali and Singh NP (2013). Antimicrobial resistance of the pathogens isolated from bovine mastitis in Goa. Ruminant Science 2(2):139-144.
Roy R, Chun J and Powell SN (2012). BRCA1 and BRCA2: Different roles in a common pathway of genome protection. Nature Reviews Cancer 12:68-78.
Ruegg PL (2005). Evaluating the effectiveness of mastitis vaccines. Resources Milk Money 3-21.
Rupp R and Boichard D (2003). Genetics of resistance to mastitis in dairy cattle. Veterinary Research 34:671-688
Sahana G, Guldbrandtsen B, Thomsen B, Holm L-E, Panitz F, Brøndum RF, Bendixen C and Lund MS (2014). Genome-wide association study using high-density singlenucleotide polymorphism arrays and whole-genome sequences for clinical mastitis traits in dairy cattle 1. Journal of Dairy Science 97:7258-7275.
Sambrook JF and Russell DW (2001). Molecular Cloning: A Laboratory Manual. 3rd Edn, Cold Spring Harbor Laboratory Press.
Trainer AH, Lewis CR, Tucker K, Meiser B and Friedlander M (2010). The role of BRCA mutation testing in determining breast cancer therapy. Nature Reviews Clinical Oncology 7:708-717
Wang F, Fang Q, Ge Z, Yu N, Xu S and Fan X (2012). Common BRCA1 and BRCA2 mutations in breast cancer families: A meta-analysis from systematic review. Molecular Biology Reports 39:2109-2118.
Wiggans GR, Van Raden PM and Cooper TA (2011). The genomic evaluation system in the United States: Past, present, future. Journal of Dairy Science 94:3202-3211.
Xu J, Wang B, Zhang Y, Li R, Wang Y and Zhang S (2012). Clinical implications for BRCA gene mutation in breast cancer. Molecular Biology Reports 39:3097-3102.
Yuan Z, Li J, Li J, Zhang L, Gao X, Gao H and Xu S (2012a). Investigation on BRCA1 SNPs and its effects on mastitis in Chinese commercial cattle. Gene 505:190-194.
Yuan Z, Li J, Li J, Zhang L, Gao X, Gao H and Xu S (2012b). BRCA1: A new candidate gene for bovine mastitis and its association analysis between single nucleotide polymorphisms and milk somatic cell score. Molecular Biology Reports 39:6625-6631.