Authors: P Mayengbam*, TC Tolenkhomba and RC Upadhyay

Source: Ruminant Science (2017)-6(1):69-74.

Cite this reference as: Mayengbam P, Tolenkhomba TC and Upadhyay RC (2017). Expression of Mn-SOD and Cu, Zn-SOD mRNA in relation to thermotolerance of Sahiwal and Karan-Fries cattle. Ruminant Science 6(1):69-74.


The present study aimed to investigate the expression of Mn-SOD and Cu, Zn-SOD mRNA in relation to physiological index of Sahiwal and Karan-Fries. Six numbers each of Sahiwal and Karan-Fries heifers in the age group of 2 to 2.5 years were exposed at 45±1oC and 50% relative humidity (RH) inside a psychrometric chamber for 4 h. Respiration rate (RR), heart rate (HR) and rectal temperature (RT) were recorded to calculate Dairy Search Index (DSI). Blood samples collected at 0, 1, 2, 3 and 4 h of heat exposure were used for isolation of lymphocytes and subsequent estimation of Mn-SOD and Cu, Zn-SOD mRNA expression by RT-PCR. RR and RT increased after 1 h in both the breeds while HR was not affected by heat exposure. Heat exposure increased DSI of Sahiwal and Karan-Fries after 2 and 1 h, respectively. Expression of Mn-SOD and Cu, Zn-SOD mRNA of both the breeds were not influenced by heat exposure. The two breeds exhibited similar RR, RT, HR, DSI, Mn-SOD and Cu, Zn-SOD mRNA expression during heat exposure.


Arya Ashwani, Verma Archana, Gupta ID, Kumar Dhaman, Magotra Ankit, Mir MA and Singh AP (2016). Influence of parity, THI and HSPB1 gene SNP on heat tolerance indicator traits in murrah buffalo (Bubalus bubalis). Ruminant Science 5(2):143-148.

Baumgard LH and Rhoads RP (2013). Effects of heat stress on post-absorptive metabolism and energetics. Annual Review of Animal Biosciences 1:311-337.

Bernabucci U, Lacetera N, Baumgard LH, Rhoads RP, Ronchi B and Nardone A (2010). Metabolic and hormonal adaptations to heat stress in domesticated ruminants. Animal 4:1167-1183.

Bernabucci U, Ronchi B, Lacetera N and Nardone A (2002). Markers of oxidative status in plasma and erythrocytes of transition dairy cows during hot season. Journal of Dairy Science 85:2173-2179.

Chauhan SS, Celi P, Fahri FT, Leury BJ and Dunshea FR (2014). Dietary antioxidants at supranutritional doses modulate skeletal muscle heat shock protein and inflammatory gene expression in sheep exposed to heat stress. Journal of Animal Science 92:4897-4908.

Corrˆea GA, Rumpf R, Mundim TCD, Franco MM and Dode MAN (2007). Oxygen tension during in vitro culture of bovine embryos: Effect in production and expression of genes related to oxidative stress. Animal Reproduction Science 67:767-775.

Duanghaklang N, Duangjinda M and Sujikara K (2015). Expression of antioxidant enzyme and heat stress genes in Thai native chicken (Chee KKU12) and Thai synthetic chicken (Khai Mook Esarn KKU50). Khon Kaen Agriculture Journal 4 (2):178-182.

Gaughan JB, Mader TL, Holt SM, Josey MJ and Rowan KJ (1999). Heat tolerance of Boran and Tuli crossbred Steers.  Journal of Animal Science 77:2398-2405.

IPCC (2007). Contribution of working groups I, II and III to the fourth assessment report of the intergovernmental panel on climate change. In: Climate Change 2007: Synthesis Report, Eds Core Writing Team, RK Pachauri and A Reisigner. Geneva, Switzerland.

Kearl LC (1982). Nutrient Requirements of Ruminants in Developing Countries. International Feedstuffs Institute, Utah Agricultural Experiment Station, Utah State University Logan, Utah USA.

Lazzari G, Wrenzycki C, Herrmann D, Duchi R, Kruip T, Niemann H and Galli C (2002). Cellular and molecular deviations in bovine in vitro-produced embryos are related to the large offspring syndrome. Biology of Reproduction 67:767-775.

Lonergan P, Rizos D, Gutie´rrez-Ada´n A, Moreira PM, Pintado B, de la Fuente J and Boland MP (2003). Temporal divergence in the pattern of messenger RNA expression in bovine embryos cultured from the zygote to blastocyst stage in vitro or in vivo. Biology of Reproduction 69:1424-1431.

Loven DP, Leeper DB and Oberley LW (1985). Superoxide dismutase levels in Chinese hamster ovary cells and ovary carcinoma cells after hyperthermia of exposure to cycloheximide. Cancer Research 45:3029-3033.

Maibam U, Hooda O K, Sarma Ph S and Upadhyay RC (2017). Seasonal variation in HSP70 expression and oxidative stress in skin of Zebu (Tharparkar) and crossbred (Karan Fries) cattle under tropical climate. Biological Rhythm Research 48(4):1-15.

Mayengbam P, Tolenkhomba TC and Upadhyay RC (2015). Mn-SOD and Cu, Zn-SOD mRNA expression in relation to physiological indices of Sahiwal and Karan-Fries heifers under different temperature humidity indices. Journal of Agrometrology 17(2):172-178.

Montilla SIR, Johnson TP, Pearce SC, Gardan-Salmon D, Gabler NK, Ross JW, Rhoads RP, Baumgard LH, Lonergan SM and  Selsby JT (2014). Heat stress causes oxidative stress but not inflammatory signaling in porcine skeletal muscle. Temperature 1(1):42-50.

Rimoldi S, Lasagna E,  Sarti FM, Marelli SP, Cozzi MC, Barnardini G and Terova G (2015). Expression profile of six stress-related genes and productive performances of fast and slow growing broiler strains reared under heat stress conditions. Meta Gene 6:17-25.

Sambrook J, Fristsch EF and Maniatis T (1989). Molecular cloning: A Laboratory Manual. 3rd Edn, Cold Spring Harbor, New York.

Scharf B, Carroll JA, Riley DG, Chase Jr CC, Coleman SW, Keisler DH, Weaber RL and Spiers DE (2010). Evaluation of physiological and blood serum differences in heat-tolerant (Romosinuano) and heat-susceptible (Angus) Bos taurus cattle during controlled heat challenge. Journal of Animal Science 88:2321-2336.

Sies H (1997). Oxidative stress: oxidants and antioxidants. Experimental Physiology 82:291-295.

St-Pierre NR, Cobanov B and Schnitkey G (2003). Economic losses from heat stress by US livestock industries. Journal of Dairy Science 86(Suppl):E52-77.

Thomas CK, Sharma KNS, Georgie GC and Razdan MN (1973). A new heat tolerance index for cattle.  Indian Journal of Animal Science 43(6):505-510.

Volodina O, Ganesan S, Pearce SC, Gabler NK, Baumgard LH, Rhoads RP and Selsby JT (2017). Short-term heat stress alters redox balance in porcine skeletal muscle. Physiogical Reports 5(8):1-10.

Yatoo MI, Dimri U and Sharma MC (2014). Seasonal changes in certain blood antioxidants in cattle and buffaloes. Indian Journal of Animal Science 84(2):173-176.