Effect of volume of flushing fluid on bacterial load in preputial wash of Murrah and Sahiwal bulls

Title: Effect of volume of flushing fluid on bacterial load in preputial wash of Murrah and Sahiwal bulls

Authors: Ankesh Kumar, JK Prasad, SK Ghosh, GK Das, S Chakravarti, HK Yadav and MR Verma

Source: Ruminant Science (2017)-6(1):169-172.

Cite this reference as: Kumar Ankesh, Prasad JK, Ghosh SK, Das GK, Chakravarti S, Yadav HK and Verma MR (2017). Effect of volume of flushing fluid on bacterial load in preputial wash of Murrah and Sahiwal bulls. Ruminant Science 6(1):169-172.


Bacteria present in the semen causes detrimental effect on the spermatozoa through toxin production and generated metabolic end products that affect the future fertility. It is now well known that preputial cavity is one of the main sources of contamination in the ejaculated semen. Despite of the fact, there is no standard methodology and recommendations to eliminate such contaminations from prepuce prior to semen collection. In context of the above mentioned facts, the study was designed to examine the effect of different volume of flushing fluid to reduce the preputial bacterial load. Total preputial capacity (100%) was determined in 11 bulls of both Murrah buffalo and Sahiwal cattle by using in-house designed Preputial Douching and Cleaning device (PDC device) and subsequently two different volumes i.e. 50% and 70% were calculated based on the full capacity keeping conventional (Syringe method) 100 ml as control. The preputial bacterial load was increased significantly (p<0.05) in bulls of both species with the increase of washing fluid volume from 100 ml to full capacity. Following washing, at day 3, the mean bacterial count in washing fluid decreased significantly (p<0.05) with the increase of washing fluid volume except full capacity volume in which the count characteristically increased (p<0.05). When bacterial load was compared on day 12 among the different methods, 70% of volume of fluid showed significantly (p<0.05) lower bacterial load indicating the superiority of this method over other methods.


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