Seasonal histoenzymic distribution of phosphatases and oxidoreductases in buffalo testis

Title: Seasonal histoenzymic distribution of phosphatases and oxidoreductases in buffalo testis

Authors: Beenish Aslam, Neelam Bansal, Varinder Uppal and Anuradha Gupta

Source: Ruminant Science (2018)-7(1):63-66.

Cite this reference as: Aslam Beenish, Bansal Neelam, Uppal Varinder and Gupta Anuradha (2018). Seasonal histoenzymic distribution of phosphatases and oxidoreductases in buffalo testis. Ruminant Science 7(1):63-66.


The present study was conducted on buffalo testis collected from the abattoirs immediately after sacrifice during each season (Spring, Summer, Autumn, Winter). The cryostat sections of 10-12 µm thickness were obtained at -23 ºC and were incubated in different substrates for demonstration of phosphatases and oxidoreductases. The study revealed a variable activity of phosphatases (AKPase, G-6-Pase) and oxidoreductases (SDH, LDH, G-6- PD, NADH AND NADPH) in different components of testis during each season of the year. The activity of different enzymes was found to be maximum during winter season, followed by autumn, spring and summer seasons which indicate more spermatogenic activity during winter as compared to other seasons.


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