Diagnosing subclinical endometritis in repeat breeder cattle using cytobrush technique

Title: Diagnosing subclinical endometritis in repeat breeder cattle using cytobrush technique

Authors: Brahmanand, Omprakash Shrivastava and Satyanidhi Shukla

Source: Ruminant Science (2018)-7(1):87-88.

Cite this reference as: Brahmanand, Shrivastava Omprakash and Shukla Satyanidhi (2018). Diagnosing subclinical endometritis in repeat breeder cattle using cytobrush technique. Ruminant Science 7(1):87-88.


Present study was conducted on 100 repeat breeder cattle to evaluate the endometrial cytology (EC) in diagnosis of subclinical endometritis. The repeat breeder cows with clear discharge were further subjected to uterine cytobrush technique for confirmation of subclinical endometritis i.e. on the basis of presence of >5 per cent polymorphnuclear (PMN) cells. The incidence of subclinical endometritis was 33 per cent (33/100). PMN percentage in the endometrial cytology samples in different groups varied from 5.67±0.21 to 7.66±0.19. It was concluded that endometrial cytology by cytobrush technique found easy and accurate method to diagnose subclinical endometritis in repeat breeder cattle.


Carneiro LC, Ferreira AF, Padua M, Saut JP, Ferraudo AS and Dos Santos RM (2014). Incidence of subclinical endometritis and its effects on reproductive performance of crossbred dairy cows. Tropical Animal Health Production 46(8):1435-1439

Dubuc J, Duffield TF, Leslie KE, Walton JS and LeBlanc SJ (2010). Definitions and diagnosis of postpartum endometritis in dairy cows. Journal of Dairy Science 93:5225-5233.

Honparkhe M, Ghuman SPS, Singh J, Dhindsa SS, Kumar A, Chandra M and Brar PS (2014). Diagnosing sub-clinical endometritis through uterine cytobrush cytology and its treatment with proteolytic enzymes in buffaloes. In: 29th Annual Convention and National Symposium of Indian Society for Study of Animal Reproduction held at Nagpur, Maharastra, 8-10 January 2014.

Kasimanickam R, Duffield TF, Foster RA, Gartely CJ, Leslie KE, Walton JS and Johnson WH (2005). A comparison of cytobrush and uterine lavage techniques to evaluate endometrial cytology in clinically normal postpartum dairy cattle. Canadian Veterinary Journal 46:255-259.

Madoz LV, Giuliodori MJ, Migliiorisi AL, Jaureguiberry M and Sota RL (2014). Endometrial cytology, biopsy and bacteriology for the diagnosis of sub-clinical endometritis in grazing dairy cows. Journal of Dairy Science 97:1-7.

Plontzke J, Madoz LV, Dela Sota RL, Heuwieser W and Drillich M (2011). Prevalence of clinical endometritis and its impact on reproductive performance in grazing dairy cattle in Argentina. Reproduction in Domestic Animals 46:520-526.

Sheldon IM, Lewis GS, LeBlanc S and Gilbert RO (2006). Defining postpartum disease in cattle. Theriogenology 65(8):1516-1530.

Snedecor GW and Cocharn WG (1994). Statistical Methods. 8th Edn, Iowa State University Press, USA.