Effect of chromium supplementation on haematobiochemical constituents and milk production in hot humid season in surti buffaloes

Title: Effect of chromium supplementation on haematobiochemical constituents and milk production in hot humid season in surti buffaloes

Authors: SS Chaudhary, VK Singh, RR Singh, SB Patel, TD Manat and LM Sorathiya

Source: Ruminant Science (2018)-7(1):97-100.

Cite this reference as: Chaudhary SS, Singh VK, Singh RR, Patel SB, Manat TD and Sorathiya LM (2018). Effect of chromium supplementation on haematobiochemical constituents and milk production in hot humid season in Surti buffaloes. Ruminant Science 7(1):97-100.


The present experiment was carried out on 18 lactating Surti buffaloes with the objective to find out the effect of dietary chromium supplementation in heat stress amelioration during hot humid season in the declining phase of lactation. These buffaloes were divided into two groups (9 animals each) i.e. control (no chromium supplementation) and treatment (chromium supplementation @ 0.5mg per kg of dry matter intake for 12 weeks. Temperature and humidity of the surrounding environment was measured and subsequently THI was calculated.  Blood samples were collected analyzed for hematological and biochemical parameters. Perusal of data revealed that after supplementation of chromium for a period of 12 weeks there was significant (P0.05) decrease in MCHC and MCH in treatment group. Rest of the hematological and biochemical parameters did not indicate any significant difference between the two groups. Significant (P0.05) differences were observed for oxidative stress parameters wherein SOD (superoxide dismutase) decreased in both, LPO (Lipid peroxide) increased in control group and GSH (Glutathione) increased in treatment groups significantly (P0.05). Milk yield was higher during most of the weeks up to 9 weeks of study in treatment group but was not significant. It can thus be concluded from the present study that supplementation of chromium in Surti buffaloes increases antioxidant defense i.e. in terms of GSH and reduces stress during hot humid season as indicated by decreased oxidative stress marker i.e. lipid peroxidation. Even though non-significant yet milk yield in declining phase of lactation showed marginally higher values upon chromium supplementation.


Al-Saiady MY, Al-Shaikh MA, Al-Mufarrej SI, Al-Showeimi, TA, Mogawer HH and Dirrar A (2004). Effect of chelated chromium supplementation on lactation performance and blood parameters of Holstein cows under heat stress. Animal Feed Science and Technology 117(3):223-233.

Bhat IH and Devi J (2014a). Influence of lactation on some enzyme activity in toggenberg goat. Ruminant Science 3(1):75-77

Bhat IH and Devi J (2014b). Study of changes in haematological parameters during different lactations in toggenberg goats. Ruminant Science 3(2):221-227.

El-Nouty FD, Al-Haidary, AA and Salah MS (1990). Seasonal variations in hematological values of high-and average-yielding holstein cattle in semi-arid environment. Journal of King Saud University 2(2):173-82.

Kegley EB, Galloway DL and Fakler TM (2000). Effect of dietary chromium-l-methionine on glucose metabolism of beef steers. Journal of Animal Science 78(12):3177-3183.

Kumar M, Kaur H, Deka RS, Mani V, Tyagi AK and Chandra G (2015). Dietary inorganic chromium in summer-exposed buffalo calves (Bubalus bubalis): Effects on biomarkers of heat stress, immune status, and endocrine variables. Biological Trace Element Research 167(1):18-27.

Lai A, Wang Z and Zhou A (2009). Effect of chromium picolinate supplementation on early lactation performance, rectal temperatures, respiration rates and plasma biochemical response of Holstein cows under heat stress. Pakistan Journal of Nutrition 8(7):940-945.

Mader TL, Davis, MS and Brown-Brand T (2006). Environmental factors influencing heat stress in feedlot cattle. Journal of Animal Science 84:712-719

Madesh M and Balasubramanian KA (1998). Microtitre plate assay for superoxidase dismutase using MTT reduction by superoxide. Indian Journal of Biochemistry and Biophysics 35:184-188.

Mc Namara JP and Valdez F (2005). Adipose tissue metabolism and production responses to calcium propionate and chromium propionate. Journal of Dairy Science 88:2498-2507.

Moron MS, Joseph WD and Bengt M (1979). Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochimica et Biophysica Acta (BBA)-General Subjects 582(1):67-78.

Pechova A, Podhorský A, Lokajova E, Pavlata L and Illek J (2002). Metabolic effects of chromium supplementation in dairy cows in the peripartal period. Acta Veterinaria Brno 71(1):9-18.

Preuss HG, Grojec PL, Lieberman S and Anderson RA (1997). Effects of different chromium compounds on blood pressure and lipid peroxidation in spontaneously hypertensive rats. Clinical nephrology 47(5):325-330.

Rehman SU (1984). Lead induced regional lipid peroxidation in brain. Toxicological letters 21:333-337.

Smith KL, Waldron MR, Drackley, JK, Socha MT and Overton TR (2005). Performance of dairy cows as affected by prepartum dietary carbohydrate source and supplementation with chromium throughout the transition period. Journal of Dairy Science 88(1):255-263.

Tezuka M, Momiyama K, Edano T and Okada S (1991). Protective effect of chromium (III) on acute lethal toxicity of carbon tetrachloride in rats and mice. Journal of Inorganic Biochemistry 42(1):1-8.

Zhang FJ, Weng XG, Wang JF, Zhou D, Zhang W, Zhai CC, Hou YX and Zhu YH (2014). Effects of temperature-humidity index and chromium supplementation on antioxidant capacity, heat shock protein 72, and cytokine responses of lactating cows. Journal of Animal Science 92(7):3026-3034.