Bilateral epididymo-orchitis caused by Brucella melitensis in a Madras red sheep

Title: Bilateral epididymo-orchitis caused by Brucella melitensis in a Madras red sheep

Authors: P Preena, UW Wasudeorao, PI Ganesan, BSM Ronald, S Balakrishnan and V Vibin

Source: Ruminant Science (2018)-7(1):145-147.

Cite this reference as: Preena P, Wasudeorao UW, Ganesan PI, Ronald BSM, Balakrishnan S and Vibin V (2018). Bilateral epididymo-orchitis caused by Brucella melitensis in a Madras red sheep. Ruminant Science 7(1):145-147.

Abstract

Present study describes bilateral epididymo-orchitis in a Madras red sheep (Ovis aries) due to Brucella melitensis infection. Clinical, serological, bacteriological and molecular investigation confirmed the presence of infection in the affected ram. A three year old Madras red ram was presented with astasis, fever, asthenia and painful bilateral enlargement of the scrotum. The ram was serologically positive for brucella antibodies by i-ELISA and RBPT. Based on isolation and identification by a battery of biochemical and agglutination tests, Brucella melitensis induced infection was established which was confirmed by Multiplex AMOS PCR assay. The ram was euthanized to prevent the spread of infection. The scrotum was examined, and the gross lesions included multiple abscesses with yellowish creamy pus, conspicuous fibro-granulomatous lesions, dense fibrous adhesions between testicular tunics and atrophy of testicular parenchyma.

 References

Abu-Seida AM, Ahmed KA, Torad FA and Marouf SA (2015). Ultrasonographic and histopathological findings in rams with epididymo-orchitis caused by Brucella melitensis. Pakistan Veterinary Journal 35(4):456-460.

Alton GG, Jones LM and Pietz DE (1975). Laboratory techniques in brucellosis. World Health Organization. Monograph series 55. 2nd Edn, WHO, Geneva.

Alton GG, Jones LM, Angus RD and Verger JM (1988). Techniques for Three Brucellosis Laboratory Institute. INRA, Puplication Paris, ISEN, France.

Bricker BJ and Halling SM (1994). Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis and Brucella suis bv. 1 by PCR. Journal of clinical microbiology 32(11):2660-2666.

Dadawala AI, Chandel BS, Chauhan HC, Kumar Pankaj, Bhagat AG and Shah NM (2012). Detection of brucella antibody by using different serological tests in cattle from north Gujarat. Ruminant Science 1(2):145-148.

Darshana UW (2014). Studies on the prevalence of brucellosis in small ruminants. MVSc Thesis submitted to TANUVAS,  Chennai.

OIE (2009). Caprine and ovine brucellosis (excluding Brucella ovis). In: Terrestrial Manual. http://www.oie.int/fileadmin/Home/eng/Health_standards /tahm/2.07. 02 _ Caprine_Ovine_Bruc.pdf, 2009 (accessed on 09.08.2016).

Panchasara HH, Patel JS and Patel PR (2015). Seroprevalence of brucella infection in organized farms and field conditions in Gujarat. Ruminant Science 4(1):37-41.

Patel KB, Patel SI, Patel BK, Chauhan HC, Bhagat AG and Chandel BS (2017). Comparative study on cultural and molecular methods for detection of Brucella melitensis in sheep and goat. Ruminant Science 6(2):309-314.

Priyanka, Shringi BN, Patel Kirit, Chauhan HC, Chandel BS and Kashyap SK (2017). Seroprevalence of brucellosis in cattle in western Rajasthan. Ruminant Science 6(2):305-307.