Genetic polymorphism of cluster of differentiation (CD) 14 gene and its association with mastitis in Deoni cattle

Title: Genetic polymorphism of cluster of differentiation (CD) 14 gene and its association with mastitis in Deoni cattle

Authors: Shivashanker, MR Jayashankar, R Nagaraja, S Yathiraj, YB Rajeshwari and Shrikrishna Isloor

Source: Ruminant Science (2018)-7(1):25-27.

Cite this reference as: Shivashanker, Jayashankar MR, Nagaraja R, Yathiraj S, Rajeshwari YB and Isloor Shrikrishna (2018). Genetic polymorphism of cluster of differentiation (CD) 14 gene and its association with mastitis in Deoni cattle. Ruminant Science 7(1):25-27.

Abstract

A study was conducted to ascertain the polymorphism of CD14 gene and its association with mastitis in Deoni cattle. Milk and blood samples were collected from 92 Deoni cattle. Milk samples were screened for subclinical mastitis using three indirect tests viz. California Mastitis Test (CMT), Electrical Conductivity and Somatic Cell Count (SCC), and grouped into mastitis and mastitis free. Genomic DNA was isolated by high salt method from blood samples, the quantity and quality of DNA was ascertained by spectrophotometer and 0.8 per cent agarose gel electrophoresis. A 832 bp fragment (part of the promoter, 5’UTR, exon 1, introns 1and part of exon 2) of CD14 gene was amplified by PCR using published primers. The PCR-RFLP method using HinfI enzyme revealed three genotypes CC, CD and DD with frequencies of 0.23, 0.47 and 0.30, respectively. The gene frequency was 0.46 and 0.54 for C and D, respectively. Chi-square analysis revealed that there is significant difference between these genotypes regarding mastitis incidence. CD genotype animals were found to be less susceptible for mastitis.

 

References

Choudhary RK, Kumar P, Sharma A and Bhattacharya TK (2014). Molecular characerization, polymorphism and association study of lactoferrin gene     with milk production and somatic cell traits in crossbred (Bos indicus x Bos taurus) cattle. Ruminant Science 3(2):157-162.

Haziot A, Ferrero E, Kontgen F, Hijiya N, Yamamoto S, Silver J, Stewart CL and Goyert SM (1996). Resistance to endotoxin shock and reduced dissemination of gram-negative bacteria in CD14-deficient mice. Immunity 4:407-414

Ibeagha-Awemu EM, Lee JW and Ibeagha AE (2008). Bovine CD14 gene characterization and relationship between polymorphisms and surface expression on monocytes and polymorphonuclear neutrophils. BMC Genetics 9:50-60.

Kumar V, Patel JS, Patel BR, Mevada VK and Raval AP (2012). Therapeutic efficacy of antimicrobial drugs in clinical mastitis of cross bred cattle. Ruminant Science 1(2):177-180.

Kumar V, Gupta ID, Verma A, Kumar SR and Chaudhari MV (2014). CD14 gene polymorphism using HinfIrestriction enzyme and its association with mastitis in Sahiwal cattle. Indian Journal of Animal Research 48:11-13

Miller SA, Dykess DD and Polesky HF (1988). A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research 16:1215

Mitra A (1994). Studies on restriction fragment length polymorphism in cattle and buffaloes. PhD thesis submitted to NDRI, Karnal, India

Selvan AS, Gupta ID, Verma A, Chaudhari MV and Kumar V (2014). Cluster of differentiation 14 gene polymorphism and its association with incidence of clinical mastitis in Karan fries cattle. Veterinary World 7(12):1037-1040

Senthil M (1995). Studies on restriction fragment length polymorphism (RFLP) in cattle. MVSc thesis submitted to TANUVAS, Chennai, India

Snedecor GW and Cochran WG (1994). Statistical Methods. 8th Edn, Iowa State University Press, Ames, Iowa.

Tizzard IR (1998). Veterinary Immunology-An Introduction. 5th Edn, Harcourt Brace and Company Asia PTE Ltd, USA.

Ulevitch RJ and Tobias PS (1995). Receptor-dependent mechanisms of cell stimulation by bacterial endotoxin. Annual Review of Immunology 13:437-457.