Title: Genetic polymorphism of cluster of differentiation (CD) 14 gene and its association with mastitis in Deoni cattle

Authors: Shivashanker, MR Jayashankar, R Nagaraja, S Yathiraj, YB Rajeshwari and Shrikrishna Isloor

Source: Ruminant Science (2018)-7(1):25-27.

Cite this reference as: Shivashanker, Jayashankar MR, Nagaraja R, Yathiraj S, Rajeshwari YB and Isloor Shrikrishna (2018). Genetic polymorphism of cluster of differentiation (CD) 14 gene and its association with mastitis in Deoni cattle. Ruminant Science 7(1):25-27.

Abstract

A study was conducted to ascertain the polymorphism of CD14 gene and its association with mastitis in Deoni cattle. Milk and blood samples were collected from 92 Deoni cattle. Milk samples were screened for subclinical mastitis using three indirect tests viz. California Mastitis Test (CMT), Electrical Conductivity and Somatic Cell Count (SCC), and grouped into mastitis and mastitis free. Genomic DNA was isolated by high salt method from blood samples, the quantity and quality of DNA was ascertained by spectrophotometer and 0.8 per cent agarose gel electrophoresis. A 832 bp fragment (part of the promoter, 5’UTR, exon 1, introns 1and part of exon 2) of CD14 gene was amplified by PCR using published primers. The PCR-RFLP method using HinfI enzyme revealed three genotypes CC, CD and DD with frequencies of 0.23, 0.47 and 0.30, respectively. The gene frequency was 0.46 and 0.54 for C and D, respectively. Chi-square analysis revealed that there is significant difference between these genotypes regarding mastitis incidence. CD genotype animals were found to be less susceptible for mastitis.

 

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