Fertility response to insulin modified Ovsynch protocol in postpartum acyclic buffaloes

Title: Fertility response to insulin modified Ovsynch protocol in postpartum acyclic buffaloes

Authors: S Singh, SN Shukla and KK Gupta

Source: Ruminant Science (2019)-8(1):45-48.

Cite this reference as: Singh S, Shukla SN and Gupta KK (2019). Fertility response to insulin modified Ovsynch protocol in postpartum acyclic buffaloes. Ruminant Science 8(1):45-48.


The fertility response to various fixed time artificial insemination protocols was studied in terms of oestrus synchronization and conception rate on sixty postpartum buffaloes. The selected buffaloes were randomly divided into five groups, each comprising twelve (n=12) animals. Buffaloes of group I received GnRH and PGF2α as per Ovsynch protocol [GnRH at day 0 and 9 and PGF2α at day 7]; group II buffaloes received treatment similar to group I except half dose of GnRH at day 9; group III buffaloes received similar treatment as group I except additional administration of insulin (0.25 IU/kg. b.wt.) on day 8, 9 and 10; group IV animals were treated similar to group III except half dose of GnRH at day 9;  group V animals were received treatment similar to group I except replacement of second GnRH injection with insulin on day 8, 9 and 10. Fixed time insemination by frozen semen was done at 60 and 72 h after PGF2α administration in all the buffaloes and pregnancy was confirmed at 60 days post insemination. The results indicated satisfactory and comparable synchronization of oestrus (83.33 to 100%) and conception rate (41.66 to 66.66%) among the various treatment groups. The fertility response following insulin modified Ovsynch based timed AI protocols in postpartum buffaloes were found quite satisfactory and comparable.


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