Title: Effect of oyster glycogen on cervical mucus microbiota and conception rate in repeat breeding cross bred cows

Authors: VL Solanki, VK Sharma, PM Chauhan, BI Prajapati, SP Desai and HC Nakhashi

Source: Ruminant Science (2019)-8(1):49-52.

Cite this reference as: Solanki VL, Sharma VK, Chauhan PM, Prajapati BI, Desai SP and Nakhashi HC (2019). Effect of oyster glycogen on cervical mucus microbiota and conception rate in repeat breeding cross bred cows. Ruminant Science 8(1):49-52.

Abstract

The microbiota of cervical mucus besides conception rate were studied following intrauterine administration of Oyster glycogen @ 500 µg in 500 ml PBS to repeat breeder cross bred cow suffered with endometritis of mild (Group I) and severe grades (Group II). Each group included 10 ailing cows while 6 repeat breeders with mild to severe grade endometritis constituted Group III to serve as control and received only PBS. The pre-treatment bacterial load (5.31±0.22 and 5. 73±0.18 x 108 cfu/ml) was higher significantly (P<0.05) in Group I and II which diminished to 0.89±0.11 and 0.97±0.07  x 108 cfu/ml at post-treatment oestrus  in both groups, respectively; whereas such a decline was absent in control group. The E. coli, Proteus, Streptococci and mixed isolates were identified in all three groups but the pre-treatment spectra of their prevalence varied with an abundance of E. coli, 40 and 30 %, followed by mixed isolates, 30 and 40%, in Group I and II, respectively which in conjuction with other isolates decreased to yield no bacterial growth in 70 and 60 percent sample at post-treatment oestrus in these two groups. Higher overall conception rate (7/10, 70%) with an optimum percentage (40%) to the first service was resulted in Group I than slightly lower percentage in Group II (6/10, 60%) than its absolute failure in control group. It is concluded that Oyster glycogen@500 µg, intra-uterine, effectively cured the endometritis of mild and severe grades in repeat breeders and also improved the conception with slightly higher rate from first service in group of mild endometritis.

References

Agarwal P, Kumar D and Prasad S (2002). Therapeutic evaluation of certain drugs for treatment of endometritis in crossbred cows. Indian Veterinary Journal 79:502-503.

Arora AK, Singh J, Pangaonkar GR and Nand AS (2000). Bacteriological studies on the genital infection in repeat breeder bovines. Indian Journal of Animal Science 15:205-207.

Bhat FA, Bhattacharyya HK and Hussain SA (2014). White side test: A simple and rapid test for evaluation of nonspecific bacterial genital infections of repeat breeding cattle. Veterinary Research Forum 5(3):177-180.

Bhuyan M, Nath KC, Deka BC, Bhuyan D and Goswami S (2015). Efficacy of E. Coli LPS and Oyster glycogen in terms of recovery and consequent conception rate in the treatment of metritis in cows. International Journal of Recent Scientific Research. 6(7):5086-5088.

Biswal SS, Das S and Mohanty DN (2013). Immunomodulatory effect of Oyster glycogen on endometritic crossbred cows. Indian Journal of Animal Reproduction 34(2):21-24.

Biswal SS, Das S, Balasubramanian S, Mohanty DN, Sethy K and Dasgupta M (2014). Serum amyloid A and haptoglobin levels in crossbred cows with endometritis following different therapy. Veterinary World 7(12):1066-1070.

Brahmanand, Shrivastava Omprakash and Shukla Satyanidhi (2018). Diagnosing subclinical endometritis in repeat breeder cattle using cytobrush technique. Ruminant Science 7(1):87-88.

Cowan ST and Steele (1975). Manual for Identification of Medical Bacteria. Cambridge University Press, London.

Dabas YPS and Maurya SN (1988). A field method for collection of bovine cervical mucus for microbiological studies. Indian Journal of Animal Reproduction 9:138-139.

Deori S (2002). Use of bacterial modulins in treatment of endometritis cows. MVSc Thesis submitted to IVRI, Izatnagar (U.P.), India.

Desai SP, Sharma VK, Chauhan PM, Sutaria TV, Suthar BN and Mody SK (2018). Gynaeco-clinical and physical properties of cervical mucus in repeat breeding crossbred cows following E.Coli LPS therapy. Ruminant Science 7(1):77-82.

Foldi JM, Kulcsar A, Pecsi B, Huyghe JA, Lohuisand G and Huszenicza (2006). Bacterial complications of postpartum uterine involution in cattle. Animal Reproduction Science 96:265-281.

Gomez KA and Gomez AA (2012). Statistical Procedure for Agricultural Research. 2nd Edn, Jhon Wieley and Sons Inc, UK.

González TM, Rodrigo Ríos R, Bacteriólogo and Salim Mattar V (2007). Prevalence of bacteria associated with infectious infertility in bovines of Monteria, Colombia. Rev. MVZ Cordoba. 12(2)

Hussain AM and Daniel RCW (1992). Effects of intrauterine infusion of Escherichia coli endotoxin in normal cows and in cows with endometritis induced by experimental infection with Streptococcus agalactiae. Theriogenology 37:791-810.

Joshi R, Mudasir M, Sharma D, Saraswat N and Singh R (2013). Bacterial microflora with repeat breeding in crossbred dairy cattle. Indian Veterinary Journal 90(6):52-54.

Madushanka DNN, Ranasingha VM, Bandara AMS, Mayurawansha WRAS and Magamage MPS (2016). Body condition score and locomotion score help to predict reproductive and health performances of dairy cattle. Ruminant Science 5(2):179-186.

Palanisamy M, EzakialNapolean R and Krishnakumar K (2015). Comparative evaluation of therapeutics for management of bovine endometritis- A study of 72 crossbred cows. Intas Polivet 16(2):218-220.

Sheldon IM, Cronin J, Goetze L, Donofrio G and Schuberth HJ (2009). Defining postpartum uterine disease and the mechanisms of infection and immunity in the female reproductive tract in cattle. Biology of Reproduction 81(6):1025-1032.

Singh J, Sidhu S, Dhaliwal G, Pangaonkar G, Nanda A and Grewal A (2000). Effectiveness of lipopolysaccharide as an intrauterine immunomodulator in curing bacterial endometritis in repeat breeding cross-bred cows. Animal Reproduction Science 59(3-4):159-166.

Sirohi NS, Monga DP and Knar SK (1989). Microbiological studies on some reproductive disorders of cattle. Indian Journal of Animal Science 59(5):537-541.

Sood P, Verma S, Katoch RC, Singh M and Vasishta NK (2003). Impact of uterine microbial panorama on the therapeutic efficacy of single injection of PGF2alpha in cows with clinical endometritis. Indian Journal of Animal Science 73(1):68-69.

Sudarshan Kumar, Bhardwaz A and Srivastava AK (2015). White side test- A field test on the cervical mucus of cows for diagnosis of endometritis. Intas Polivet 16(2):207-213.

Wakayo BU, Brar PS, Prabharkar S and Arora AK (2015). Evaluation of prophylactic cloprostenol and E. coli LPS treatment against endometritis in dairy cows and buffaloes. Journal of Reproduction and Infertility 6(1):17-21.