Title: Supra-nutritional trace elements affect ruminal protein metabolism in buffalo (Bubalus bubalis) bulls
Authors: Vishal Mudgal, Anil Kumar Garg and Ram Sharan Dass
Source: Ruminant Science (2019)-8(1):9-12
Cite this reference as: Mudgal Vishal, Garg AK and Dass RS (2019). Supra-nutritional trace elements affect ruminal protein metabolism in buffalo (Bubalus bubalis) bulls. Ruminant Science 8(1):9-12.
An experiment was conducted on 4 rumen fistulated buffalo bulls (aged about 4 years, mean body weight 352 kg) in 4 x 4 Latin square switchover design, to elucidate the effect of supra-nutritional levels of copper (Cu) or /and selenium (Se). Different groups were fed either targeted minerals sufficient (Cu 10.02 ppm and Se 0.22 ppm) control (C) diet or supplemented with 10 ppm Cu (T1), 0.3 ppm Se (T2) and both trace elements simultaneously (T3). Bulls were fed on wheat straw and concentrate mixture diet to meet their nutrient requirements. After 21 days of feeding on a particular diet, and before switching the animals to the next diet, rumen liquor was collected for three consecutive days from all the animals at zero (pre-prandially), 2, 4, 6 and 8-hour post-prandially to measure different ruminal nitrogen fractions in buffalo bulls. Results revealed no significant effect of treatments on ammonical nitrogen (N), total-N, trichloroacetic acid (TCA)- precipitable-N and non-protein-N in rumen liquor of buffalo bulls. Overall mean concentrations of total-N were found to be significantly (P<0.05) higher at 2 hours, as compared to 0, 6 and 8 hours, whereas the ammonia nitrogen concentration was significantly lower (P<0.01) at 0 and 8 hours as compared to 2 hours. Values at 0 hours were also lower (P<0.05) as compared to 4 and 6 hours. The non-protein-N values were found to be significantly (P<0.01) higher at 2 hours as compared to 0, 6 and 8 hours.
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