10-Title: Assessment of comparative efficacy of drugs against benzimidazole resistance in gastrointestinal nematodes of goats
Authors: Kusum Lata, G Das, NK Kumbhakar and S Nath
Source: Ruminant Science (2020)-9(1):49-54.
How to cite this manuscript: Lata Kusum, Das G, Kumbhakar NK and Nath S (2020). Assessment of comparative efficacy of drugs against benzimidazole resistance in gastrointestinal nematodes of goats. Ruminant Science 9(1):49-54.
The present investigation was undertaken to study the status of Benzimidazole (BZ) resistance in caprine nematodes of Jabalpur, Madhya Pradesh and to study the efficacy of Fenbendazole (FBZ) with Piperonyl Butoxide (PBT) and Methimazole (MTH) against caprine GI nematodes. Prevalence of BZ resistance, detected by in-vivo faecal egg count reduction test (FECRT), revealed goats reared in farm condition were resistance as compared to the field. In-vitro egg hatch assay (EHA) also revealed resistance in strongyles of the farm as compared to field conditions. Log probit analysis with ED50 value of 0.149-0.238 µg TBZ/ml in farm and 0.003 – 0.053 µg TBZ/ml in the field, confirmed the existence of BZ resistant nematodes in farm conditions. Haemonchus contortus was the dominant nematode exhibiting resistance to BZ treatment followed by Trichostrongylus sp. and Strongyloides sp.
In order to study the comparative efficacy, the farm goats showing resistance to BZ anthelminthic were treated with FBZ+PBT @ 7.5 mg/kg and 63 mg/kg b.wt. and [email protected] 7.5 mg/kg and 3 mg/kg b.wt., respectively. The rates of reduction were higher for FBZ+PBT treated goats with 98.5% FECR as compared to FBZ+MTH and only FBZ treated goats with 65.60% and 80.60% FECR, respectively indicated high efficacy of FBZ+PBT combination against resistant GI nematode in comparison to FBZ treatment. Results of in vitro EHA corroborating with that of FECRT by showing high efficacy of FBZ+PBT combination. To conclude, the prevalence of BZ resistance was detected against caprine GI nematodes in farm conditions and can be successfully treated by a combination of Fenbendazole and Piperonyl Butoxide.
Assis LM, Bevilaqua CML, Morais SM, Vieira LS, Costa CTS and Souza JAL (2003). Ovicidal and larvicidal activity in vitro of Spigelia anthelmia Linn extracts on Haemonchus contortus. Veterinary Parasitology 117:43-49.
Benchaoui HA and Mckellar QA (1996). Interaction between fenbendazole and piperonyl butoxide: pharmacokinetic and pharmacodynamic Implications. Journal of Pharmacy and Pharmacology 48:753-759.
Chartier C, Pors I, Hubert J, Rocheteau D, Benoit C and Bernard N (1998). Prevalence of anthelmintic resistant nematodes in sheep and goats in western France. Small Ruminant Research 29:33-41.
Coles GC, Bauer C, Borgsteede FHM, Geerts S, Klei TR, Taylor MA and Waller PJ (1992). World Association for the Advancement of Veterinary Parasitology (WAAVP) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Veterinary Parasitology 44:35-44.
Coles GS, Jackson F, Pomroy WE, Prichard RK, Von Samson-Himmelstjerna G, Silvestre A, Taylor MA and Vercruysse J (2006). The detection of anthelmintic resistance in nematodes of veterinary importance. Veterinary Parasitology 136:167-185.
Das G, Dixit AK, Nath S, Agrawal V and Dongre S (2015). Levamisole and fenbendazole resistance among gastrointestinal nematodes in goats at Jabalpur, Madhya Pradesh. Journal of Veterinary Parasitology 29:98-102.
Das M and Singh S (2005). Anthelmintic resistance to nematodes in sheep and goat farms in Hissar. Journal of Veterinary Parasitology 19:103-106.
Dixit AK (2016). Detection of benzimidazole resistance in Haemonchus contortus of goats. PhD thesis submitted to NDVSU, Jabalpur.
Gill BS (1996). Anthelmintic resistance in India. Veterinary Parasitology 63:173-176.
Gillham RJ and Obendorf DL (1985). Therapeutic future of levamisole in dairy goats. Australian Veterinary Journal 62:426-427.
Hall CA, Campbell NJ and Carroll SN (1978). Resistance to thiabendazole in a field population of Ostertagia circumcincta from sheep. Australian Veterinary Journal 55:5-7.
Jackson F (1993). Anthelmintic resistance-The state of play. British Veterinary Journal 49:123-128.
Jackson F and Coop RL (2000). The development of anthelmintic resistance in sheep nematodes. Parasitology 120:95-107.
Kumbhakar NK, Sanyal PK, Rawte D, Kumar D, Kerketta AE and Pal S (2015). Efficacy of pharmacokinetic interactions between piperonyl butoxide and albendazole against gastrointestinal nematodiasis in goats. Journal of Helminthology 90:624-629.
Le Jambre LF (1976). Egg hatch as an in vitro assay of thiabendazole resistance in nematodes. Veterinary Parasitology 2:385-391.
Martin PJ and Wursthorn L (1991). RESO faecal egg count reduction test calculator. Council of Scientific and Industrial Research Organization, Division of Animal Health, Melbourne, Australia.
Sanyal PK (1998). Effect of single and divided dose administration on the pharmacokinetics of albendazole in sheep and goat. Veterinary Journal 155:311-316.
Shahardar RA, Itoo SA, Allaie IM and Wani ZA (2014). Anthelmintic resistance in GI nematodes of sheep at farms of Kashmir valley. Journal of Veterinary Parasitology 28:151-153.
Taylor MA, Hunt KR and Goodyear KL (2002). Anthelmintic resistance detection methods. Veterinary Parasitology 103:183-194.
Van Wyk JA, Cabaret J and Michael LM (2004). Morphological identification of nematode larvae of small ruminants and cattle simplified. Veterinary Parasitology 119:277-306.
Waller PJ (1987). Anthelmintic resistance and the future for roundworm control. Veterinary Parasitology 25:177-191.