10-Title: Assessment of comparative efficacy of drugs against benzimidazole resistance in gastrointestinal nematodes of goats
Authors: Kusum Lata, G Das, NK Kumbhakar and S Nath
Source: Ruminant Science (2020)-9(1):49-54.
How to cite this manuscript: Lata Kusum, Das G, Kumbhakar NK and Nath S (2020). Assessment of comparative efficacy of drugs against benzimidazole resistance in gastrointestinal nematodes of goats. Ruminant Science 9(1):49-54.
The present investigation was undertaken to study the status of Benzimidazole (BZ) resistance in caprine nematodes of Jabalpur, Madhya Pradesh and to study the efficacy of Fenbendazole (FBZ) with Piperonyl Butoxide (PBT) and Methimazole (MTH) against caprine GI nematodes. Prevalence of BZ resistance, detected by in-vivo faecal egg count reduction test (FECRT), revealed goats reared in farm condition were resistance as compared to the field. In-vitro egg hatch assay (EHA) also revealed resistance in strongyles of the farm as compared to field conditions. Log probit analysis with ED50 value of 0.149-0.238 µg TBZ/ml in farm and 0.003 – 0.053 µg TBZ/ml in the field, confirmed the existence of BZ resistant nematodes in farm conditions. Haemonchus contortus was the dominant nematode exhibiting resistance to BZ treatment followed by Trichostrongylus sp. and Strongyloides sp.
In order to study the comparative efficacy, the farm goats showing resistance to BZ anthelminthic were treated with FBZ+PBT @ 7.5 mg/kg and 63 mg/kg b.wt. and FBZ+MTH@ 7.5 mg/kg and 3 mg/kg b.wt., respectively. The rates of reduction were higher for FBZ+PBT treated goats with 98.5% FECR as compared to FBZ+MTH and only FBZ treated goats with 65.60% and 80.60% FECR, respectively indicated high efficacy of FBZ+PBT combination against resistant GI nematode in comparison to FBZ treatment. Results of in vitro EHA corroborating with that of FECRT by showing high efficacy of FBZ+PBT combination. To conclude, the prevalence of BZ resistance was detected against caprine GI nematodes in farm conditions and can be successfully treated by a combination of Fenbendazole and Piperonyl Butoxide.
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