11-Title: Detection of Trypanosoma evansi in cross breed cattle of North Gujarat

Authors: JG Patel, SH Raval, RS Parmar, SS Patel, RL Patel, BJ Patel and DV Joshi

Source: Ruminant Science (2020)-9(1):55-58.


How to cite this manuscript: Patel JG, Raval SH, Parmar RS, Patel SS, Patel RL, Patel BJ and Joshi DV (2020). Detection of Trypanosoma evansi in cross breed cattle of North Gujarat. Ruminant Science 9(1):55-58.


Present study was carried out on 997 cross breed cattle with recurrent fever, muscular weakness, anaemia and loss of appetite. Out of 997 samples, 116 were found anaemic (Hb<8.5) through haematological examination. Blood smear examination and polymerase chain reaction (PCR) were performed on 116 blood samples to detect trypanosoma. Examination of blood well as buffy coat smear(s) revealed that only 11 (9.48%) were found positive for Trypanosoma, while 37 (31.90%) out of 116 animals were found positive in polymerase chain reaction (PCR) analysis. Hence, PCR is considered the most suitable diagnostic and confirmatory test for early diagnosis of trypanosomosis infection and consequently controlling programs.


Bal MS, Sharma A, Ashuma Bath BK, Kaur P and Singla LD (2014). Detection and management of latent infection of Trypanosoma evansi in a cattle herd. Indian Journal of Animal Research 48(1):31-37.

Baticados WN, Castro DL and Baticados AM (2011a). Parasitological and PCR detection of Trypanosoma evansi in buffaloes from Luzon, Philippines. Ceylon Journal of Science (Biological Sciences) 40(2):141-146.

Baticados WN, Fernandez CP and Baticados AM (2011b). Molecular detection of Trypanosoma evansi in cattle from Quirino Province, Philippines. Veterinarski Arhiv 81(5):635-646.

Beltran MA, Copeman B, Copland R and Reid S (2004). PCR-ELISA for detection of Trypanosoma evansi in tissues in Buffaloes. Proceedings of 7th World Buffalo Congress, Makati City, Philippines, pp 89-90.

Desquesnes M and Davila AMR (2002). Applications of PCR-based tools for detection and identification of animal trypanosomes: A review and perspectives. Veterinary Parasitology 109(3-4):213–231.

Hilali M, Abdel-Gawad A, Nassar A and Abdel-Wahab A (2006). Haematological and biochemical changes in water buffalo calves (Bubalus bubalis) infected with Trypanosoma evansi. Veterinary Parasitology 139(1-3):237-243.

Martin SW (1977). The evaluation of tests. Canadian Journal of Comparative Medicine 41:19-25.

Moolchandani A and Sareen M (2015). Influence of trypanosomiasis on blood biochemical indices of camel (Camelus dromedarius). Ruminant Science 4(2):205-206.

Mugittu KN, Silayo RS, Majiwa PAO, Kimbita EK, Mutayoba BM and Maselle R (2001). Application of PCR and DNA probes in the characterisation of trypanosomes in the blood of cattle in farms in Morogoro, Tanzania. Veterinary Parasitology 94(3):177-189.

Nantulya V (1990). Trypanosomiasis in domestic animals: the problems of diagnosis. Revue Scientifique et Technique (International Office of Epizootics) 9(2):357-367.

Parashar R, Singla LD, Batra K, Kumar R, Kashyap N, Kaur P and Bal MS (2018). Unraveling cryptic epizootiology of equid trypanosomosis in Punjab state of India by parasitological and sero-molecular techniques. Acta Tropica 185:18-26.

Parashar Rahul and Singla LD (2019). Challenges in control of trypanosomosis in ruminants: Alarming bells on drug resistance. Ruminant Science 8(2):153-162.

Ponnudurai G, Sivaraman S, Rani N and Veerapandian C (2015). An outbreak of trypanosomosis in buffaloes caused by diminazene resistant Trypanosoma evansi. Buffalo Bulletin 34(1):1-4.

Shehu SA, Ibrahim NDG, Esievo KAN and Mohammed G (2006). Role of erythrocyte surface sialic acid in inducing anaemia in Savannah brown bucks experimentally infected with Trypanosoma evansi. Veterinarski Arhiv 76(6):521-530.

Singh Veer and Tiwari AK (2012). Bovine surra in India: An update. Ruminant Science 1(1):1-7.

Singla LD, Juyal PD and Sharma NS (2010). Immune responses to haemorrhagic septicaemia (HS) vaccination in Trypanosoma evansi infected buffalo-calves. Tropical Animal Health and Production 42:589-595.

Walia PS, Kalra IS, Juyal PD and Ahuja SP (1996). Role of activity of Trypanosoma evansi in inducing anemia and immunomodulation in buffalo calves. Journal of Veterinary Parasitology 10(1):1-9.