16-Title: Reproductive response and progesterone profile of aged Corriedale ewes following different hormone based oestrous synchronization schemes

Authors: F Rasool, FA Lone and MN Banday

Source: Ruminant Science (2020)-9(1):77-82.

 

How to cite this manuscript: Rasool F, Lone FA and Banday MN (2020). Reproductive response and progesterone profile of aged Corriedale ewes following different hormone based oestrous synchronization schemes. Ruminant Science 9(1):77-82.

Abstract

The aim of this study was to determine the impact of various oestrous synchronization schemes on the reproductive response and progesterone (P4) profile of aged Corriedale ewes. Twenty four (n=24) healthy aged Corriedale ewes (8-9 years) were selected randomly and equally divided into three groups designated as SP, SPG and SE. Ewes in group SP and SPG received intravaginal P4 sponges for a period of 9 days followed by an intramuscular injection of PGF2alpha (250 µg Cloprostenol sodium; Pragma-Intas India) at 24 hours before sponge. However, the SPG group did also receive an intramuscular injection of GnRH (8.2 µg Buserelin acetate; Receptal Vet-MSD India)) on day 12 post breeding. Ewes in group SE also received intravaginal P4 sponges for a period of 9 days followed by an intramuscular injection of eCG (500IU; Folligon, Intervet India) at the time of sponge removal. The pregnancy rate (PR) and lambing rate (LR) were significantly (P<0.05) higher in the SP group as compared to SPG and SE. Time of blood collection affected P4 concentration which was significantly (P<0.05) higher at day 35 in the SP group than SE but non-significantly (P>0.05) higher than the SPG group. Pooled P4 concentration was significantly (P<0.05) higher in pregnant compared with non-pregnant ewes at day 15 and 35. Further, significant (P<0.05) decline in serum P4 concentration was observed from day 0 to 35 in non-pregnant ewes, from day 0 to breeding in pregnant ewes followed by significant (P<0.05) increase from breeding to day 15 and 35. The scheme of oestrous synchronization used in the SP group showed better fertility in older ewes, hence could be used as a valuable tool for better reproductive outcome in aged ewes.

References

Anghel A, Zamfirescu S, Coprean D, Elena S and Dobrin N (2011). Assessment of progesterone and pregnancy associated glycoprotein concentrations for early pregnancy diagnosis in ewe. Annals of the Romanian Society for Cell Biology 16(2):133-136.

Ataman MB, Akoz M and Akman O (2006). Induction of synchronized oestrus in akkaraman cross-bred ewes during breeding and anestrus seasons, the use of short-term and long-term progesterone treatments. Revue de Médicine Vétérinaire 157(5):257-260.

Ataman MB, Aköz M, Saribay MK, Erdem H and Bucak MN (2013). Prevention of embryonic death using different hormonal treatments in ewes. Turkish Journal of Veterinary and Animal Sciences 37:6-8.

Clemente M, La Fuente T, Fair T, Al Naib A, Gutierrez-Adan A, Roche JF, Rizos D and Lonergan P (2009). Progesterone and conceptus elongation in cattle, a direct effect on the embryo or an indirect effect via the endometrium? Reproduction 138:507-517.

Dudi V, Mehta JS, Chaudhary AK, Kumar Pramod, Kumar Amit and Ruhil Swati (2017). Efficacy of different estrus synchronizing protocols on estrus induction in postpartum lactating dairy cows. Ruminant Science 6(2):337-340.

El-Tahawy Abdelgawad Salah and Mostafa Ibrahim Ahmed (2015). Prevalence, risk factors and cross sectional epidemiology for some selected diseases and syndromes affecting Rahmani sheep with particular spotlight on their economic consequences. Ruminant Science 4(2):159-165.

Fukui Y, Ishikawa D, Ishida N, Okada M, Itagaki R and Ogiso T (1999). Comparison of fertility of estrous synchronized ewes with four different intravaginal devices during the breeding season. Journal of Reproduction and Development 45:337-343.

Gardón JC, Escribano B, Astiz S and Ruiz S (2015). Synchronization protocols in Spanish Merino sheep, reduction in time to estrus by the addition of eCG to a progesterone-based estrus synchronization protocol. Annals of Animal Science 15:409-418.

Hasani N, Ebrahimi M, Ghasemi-Panahi B and Khani AH (2018). Evaluating reproductive performance of three estrus synchronization protocols in Ghezel ewes. Theriogenology 122:9-13.

Hashem NM, El-Azrak KM, Nour El-Din AN, Taha TA and Salem MH (2015). Effect of GnRH treatment on ovarian activity and reproductive performance of low-prolific Rahmani ewes. Theriogenology 83:192-198.

Jackson CG, Neville TL, Mercadante VRG, Waters KM, Lamb GC, Dahlen CR and Redden RR(2014). Efficacy of various five-day oestrous synchronization protocols in sheep. Small Ruminant Research 120:100-107.

Karsch FJ, Legan SJ, Ryan KD and Foster DL (1980). Importance of estradiol and progesterone in regulating LH secretion and estrous behavior during the sheep estrous cycle. Biology of Reproduction 23:404-413.

Kumar Amit, Mehta JS, Ruhil Swati and Purohit GN (2018). Effects of different estrous synchronization protocols on estrus and subsequent fertility in cycling cows. Ruminant Science 7(1):83-86.

 Lone FA, Malik AA, Khatun A, Islam R, Khan HM and Shabir M (2016). Returning of cyclicity in infertile Corriedale sheep with natural progesterone and GnRH based strategies. Asian Pacific Journal of Reproduction 5:64-67.

Lone FA (2013). Concepts and physiological understanding of endocrine and ovarian events in relation to an ovarian cycle in cattle. Research and Reviews-Journal of Veterinary Science and Technology 1-9.

Martemucci G and D’Alessandro AG (2010). Synchronization of oestrus and ovulation by short time combined FGA, PGF2alpha, GnRH, eCG treatments for natural service or AI fixed-time. Animal Reproduction Science 123:32-39.

Martinez MF, McLeod B, Tattersfield G, Smaill B, Quirke LD and Juenge JL (2015). Successful induction of oestrus, ovulation and pregnancy in adult ewes and ewe lambs out of the breeding season using a GnRH+progesterone oestrus synchronisation protocol. Animal Reproduction Science 155:28-35.

McCappin N and Murray RD (2011). Factors affecting the pregnancy rate in ewes following AI. Veterinary Record 168:99.

McNatty KP, Smith P, Hudson NL, Heath DA and Tisdall DJO (1995). Development of the sheep ovary during fetal and early neonatal life and the effect of fecundity genes. Journal of Reproduction and Fertility 49:123-135.

Olivera-Muzante J, Fierro S, López V and Gil J(2011). Comparison of prostaglandin and progesterone-based protocols for timed artificial insemination in sheep. Theriogenology 75:1232-1238.

Santolaria P, Palacin I and Yaniz J (2011). Management factors affecting fertility in sheep. In: Artificial Insemination in Farm Animals. Eds: M Manafi. Intech Open Limited, London Bridge Street London, United Kingdom.

Sawalha MN, Kridli RT, Jawasreh KI and Meza-Herrera CA (2011). The use of melatonin and progestageneeCG to initiate reproductive activity in prepuberal Awassi ewe lambs. Tropical Animal Health and Production 43:1345-50.

Selaive-Villarroel AB and Kennedy JP (1983). Fertility studies in young and mature Merino ewes, cervical mucus production. Theriogenology 20:537-541.

Shakya NK, Shukla SN, Shrivastava OP and Inwati P (2019). Reproductive performance of goats under field condition in Jabalpur. Ruminant Science 8(2):189-190.

Shukla MK and Garg Abhishek (2012). Efficacy of different route of administration and doses of dinoprost in estrus induction/synchronization in subestrus Murrah buffaloes. Ruminant Science 1(1):63-65.

Simonetti L, Blanco MR and GardoÂn JC (2000). Estrus synchronization in ewes treated with sponges impregnated with different doses of medroxyprogesterone acetate. Small Ruminant Research 38:243-247.

Singh S, Shukla SN and Gupta KK (2019). Fertility response to insulin modified Ovsynch protocol in postpartum acyclic buffaloes. Ruminant Science 8(1):45-48.

Ungerfeld R and Rubianes E(2002). Short term primings with different progestagen intravaginal devices (MAP, FGA, and CIDR) for eCG-estrous induction in anestrus ewes. Small Ruminant Research 46:63-66.