1-Title: Sperm phenotype and flowcytometry parameters in productive buffalo bulls of different age
Authors: KV Jayanthi, GS Naveen Kumar, M Ashokan, K Hemanth Gowda, D Ramesh, Shivan Gouda Patil and A Kumaresan
Source: Ruminant Science (2023)-12(2):185-190.

Abstract

How to cite this manuscript: Jayanthi KV, Kumar GS Naveen, Ashokan M, Gowda K Hemanth, Ramesh D, Patil Shivan Gouda and Kumaresan A (2023). Sperm phenotype and flowcytometry parameters in productive buffalo bulls of different age. Ruminant Science 12(2):185-190.
Abstract
The present study aimed to analyze sperm phenotypic and flow cytometry parameters and their association with age among healthy breeding Murrah buffalo bulls. Data on sperm phenotypic characters of twenty Murrah buffalo bulls of different ages and in semen production stationed at Nandini Sperm Station, Bengaluru were analyzed immediately after semen collection, and semen straws of the same bulls were subjected to semen flow cytometry analysis. Buffalo bulls were divided into three groups based on age G-I (33-36 months), G-II (49-63months), and G-III (72-113 months). The mean±SE of semen phenotypic characters ejaculate volume, semen pH, sperm concentration, initial motility, and post-thaw motility were 3.867±0.059 ml, 6.479±0.017, 1347±65.84 (106/ml), 77.778±1.22 (%) and 41.627±1.65 (%), respectively. A significantly higher ejaculate volume, pH, post-thaw motility and lower sperm concentration with age was observed. The mean flow cytometry parameter values were sperm acrosomal integrity (LAI% (44.93±2.94), LAR% (8.49±0.51), DAI% (7.81±0.62) and DAR % (38.11±2.61)), plasma membrane integrity (total live % 48.20±3.10 and dead % 42.83±4.65), mitochondrial reactive oxygen species (62.69±2.66 %) and intracellular calcium concentration (LLC % (16.30±1.37), LHC % (38.27±2.52), DLC % (34.39±2.68) and DHC % (11.03±0.87)). However, flow cytometric parameters were not significantly associated with age among productive healthy bulls.
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