10-Title: Occurrence of antibiotic resistance genes in Escherichia coli isolated from diarrhoeic and healthy camel calves
Authors: Kirti Suthar, Shirish Dadarao Narnaware, Rakesh Ranjan, Anil Moolchandani and Narendra Singh Rathore
Source: Ruminant Science (2024)-13(1):65-69.

Abstract

How to cite this manuscript: Suthar Kirti, Narnaware Shirish Dadarao, Ranjan Rakesh, Moolchandani Anil and Rathore Narendra Singh (2024). Occurrence of antibiotic resistance genes in Escherichia coli isolated from diarrhoeic and healthy camel calves. Ruminant Science 13(1):65-69.
Abstract
A total of 51 rectal swab samples were collected from apparently healthy (n=39) as well as diarrhoeic (n=12) camel calves from an organized camel farm of Bikaner, Rajasthan. Bacterial culture revealed isolation of E. coli colonies from 14 (35.89%) healthy and 12 (100%) diarrhoeic camel calves. From these colonies extraction of bacterial DNA was performed. Detection of different antibiotic resistance genes encoding extended-spectrum â lactamase (ESBL) (blaTEM, blaSHV, blaOXA-1, blaCTX-M-1, blaCMY-2, blaKPC and blaOXA-48), colistin resistance (mcr-1), class 1 integron-integrase (int1) and tetracycline resistance (tetA, tetB) from E. coli isolates was carried out by PCR. Screening for different antibiotic resistance genes in E. coli isolates revealed the highest prevalence of tetA gene (73.06%) followed by int1 (69.23%), blaCTX-M-1 (65.38%), tetB (61.53%), blaTEM (57.69%), mcr-1 (42.30%), blaOXA-48 (38.46%), blaSHV (30.76%), blaOXA-1 (30.76%), blaCMY-2 (26.92%) and blaKPC (0%) genes. This study reveals a high percentage of camel calves as carriers of tetracycline resistance, ESBL producing and colistin resistance genes in India which can contribute to the dissemination of multidrug-resistant E. coli between the three axes of one health i.e. animal, human and environment.
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