12-Title: Etiopathology of diarrhoea in goats and antimicrobial resistance of bacterial isolates
Authors: Ritesh Limbat, Mamta Kumari, Rajesh Singathia and Sandhya Morwal
Source: Ruminant Science (2024)-13(1):75-86.

Abstract

How to cite this manuscript: Limbat Ritesh, Kumari Mamta, Singathia Rajesh and Morwal Sandhya (2024). Etiopathology of diarrhoea in goats and antimicrobial resistance of bacterial isolates. Ruminant Science 13(1):75-86.
Abstract
Goat farming contributes significantly to the nutrition and income of rural people but goats are commonly affected with diarrhea. The present investigation was conducted to study the etiopathology of the diarrheic condition of goats in the Southern region of Rajasthan. Out of total 80 cases examined, 35 goats were having clinical diarrhea. Bacterial isolation and antibiotic sensitivity patterns were done along with the study of pathological changes in various organs. Bacterial isolation revealed the presence of E.coli (40%), Pseudomonas aeruginosa (16.66%), Proteus vulgaris (23.33%) and Citrobacter koseri (20%). The different serotypes of E.coli identified were O26, O98, O7 and O17. Antibiotic sensitivity patterns of various bacterial isolates revealed that Amikacin followed by Streptomycin and Cefixime are the most sensitive drugs. However, Rifampicin, Azithromycin and Tetracycline were showing resistance to two or more bacterial isolates. The uspA and Oprl genes of E.coli and Pseudomonas aeruginosa, respectively were successfully amplified using species-specific primers. Major pathological lesions included the presence of congestion and haemorrhage in the intestine and abomasum. A thin fibrinous layer and necrotic foci were seen in the liver. In the histopathological investigation, various lesions recorded were congestion, haemorrhage, oedema, necrosis, degeneration and infiltration of leukocytes. Escherichia coli was found to be the predominant bacteria affecting goats. It had the highest sensitivity to Amikacin. The major pathological lesions encountered were hepatitis, enteritis and abomasitis.
References
Abdullah FFA, Chung ELT, Sabrina L, Abba Y, Sadiq MA, Mohammed K, Hambali IU, Bitrus AA, Haron AW and Lila MA (2016). Clinical case of severe anaemia in a sheep due to parasitic gastroenteritis (PGE) infection concurrent with eperythrozoonosis. International Journal of Livestock Research 4(2):75-78.
Agrawal Himanshu, Tripathi Arvind Kumar, Srivastava Ashish, Sharma Ambika, Panigrahi PN, Jaiswal Manu and Dhuriya Shubhasini (2021). Evaluation of therapeutic potential of Dalbergia sissoo and Punica granatum on clinical cases of calf diarrhoea. Ruminant Science 10(2):367-374.
Ali ME, Sujan KM, Rasel IH and Islam MK (2021). Prevalence of diarrhoea of goats and their concurrent blood profile in North West region of Bangladesh. Asian Journal of Medical and Biological Research 7(1):6-11.
Amadi VA, Avendano E, Onyegbule OA, Pearl Z, Graeme S, Sharma R and Hariharan H (2015). Antimicrobial drug resistance in Escherichia coli including an O157: H7 isolate from feces of healthy goats in Grenada. Annual Research and Review in Biology 68-74.
Asopa Shesh, Vyas I, Dadhich H, Joshi A and Rani S (2021). Haematobiochemical alterations associated with enteritis in dromedary camels (Camelus dromedarius). Ruminant Science 10(2):363-366.
Basic Animal Husbandry Statistics (2019). Department of Animal Husbandry and Dairying Ministry of Fisheries, Animal Husbandry and Dairying Goat of India New Delhi. Retrieved from dahdnicin /circulars/basic-animal-husbandry-statistics-2019
Bauer AW, Kirby MM, Sherris JC and Truck M (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology 45:493-496.
Begum F, Islam MM, Sohidullah M, Kabir SML, Islam M and Rahman MT (2016). Molecular identification and antibiogram profiles of Escherichia coli isolated from apparently healthy and diarrheic goats. Bangladesh Journal of Veterinary Medicine 14(2):203-208.
Bhati Anita, Shringi Archana, Vyas Indu, Mandovera Vijay and Rani Sunita (2013). A study of pathological conditions in buffalo intestine. Ruminant Science 2(1):43-44.
Carter ME, Quinn PJ, Markey B and Carter GR (2004). Text Book of Clinical Veterinary Microbiology. Elsevier, 1-646.
Cid D, Piriz S, Ruiz Santa, Quiteria JA, Valle J, Vadillo S and De La Fuente R (1996). In vitro susceptibility of Escherichia coli strains isolated from diarrhoeic lambs and goat kids to 14 antimicrobial agents. Journal of Veterinary Pharmacology and Therapeutics 19(5):397-401
Cruickshank R, Duguid JP, Marsion BP and Swain RHA (1975). Medical-Microbiological. Volume II 12th Edn, Churchill Livingstone, Edinburgh, London and New York.
Dadawala AI , BS Chandel, HC Chauhan and AG Bhagat (2013). Molecular detection of bovine rotavirus from north Gujarat, India. Ruminant Science 2(2):131-137.
Dapgh AN, Hakim AS, Abouelhag HA, Abdou AM and Elgabry EA (2019). Detection of virulence and multidrug resistance operon in Pseudomonas aeruginosa isolated from Egyptian Baladi sheep and goat. Veterinary world 12(10):1524-1528
Dhaka Prachi, Gottam GS, Gupta Barkha, Meharania Smeer and Maida Sushila (2023). Physiological and haematological alterations in diarrhoeic cattle calves during different seasons. Ruminant Science 12(2):347-354.
Dohare AK, Singh B, Bangar Y, Prasad S, Kumar D and G Shakya (2013) Influence of age, sex and season on morbidity and mortality pattern in goats under village conditions of Madhya Pradesh. Veterinary World 6(6):329-331.
El-Tahawy Abdelgawad Salah and Mostafa Ibrahim Ahmed (2015). Prevalence, risk factors and cross sectional epidemiology for some selected diseases and syndromes affecting Rahmani sheep with particular spotlight on their economic consequences. Ruminant Science 4(2):159-165.
Ershaduzzaman M, Rahman MM, Roy BK and Chowdhury SA (2007). Studies on the disease and mortality pattern of goats under farm condition and some factors affecting mortality and survival rates in black Bengal kids. Bangladesh Journal of Veterinary Medicine 5(1-2):71-76.
Ershaduzzaman Md, Das T, Haque Md M and Rahman M (2013). Concurrent infection of gastro intestinal parasites and bacteria associated with diarrhoea in Bengal goats in Bangladesh. International Journal of Innovation and Applied Studies 3:449-455.
Fuente R, Garcia S, Orden A, Ruiz A, Diez R and Cid D (2002). Prevalence and characteristics of attaching and effacing strains of Escherichia coli isolated from diarrheic and healthy sheep and goats. American Journal of Veterinary Research 63:262-266.
Islam S, Akhand MRN, Maruf BK and Mohammed KH (2021). Prevalence of the infectious diseases of goats in Sylhet District, Bangladesh. Scholars Journal of Agriculture and Veterinary Sciences 8(4):42-50
Jesse F, Chung L, Teik E and Abba Y (2019). A veterinary clinical case of severe chronic Haemonchus contortus infection in a goat. Advance in Animal and Veterinary Science 7(6):503.
Jolhe DK, Gumasta Poornima, Ghosh RC, Giri Devesh Kumar, Sannat Chandrahas, Argade Shubhangi and Prajapati Deepak (2019). Pathomorphological study of coccidiosis in goats (Capra hircus). Ruminant Science 8(2):221-224.
Kapadiya DK, Joshi DV, Chaudhary SS, Shah NM, Panchasara HH and Patel BJ (2012a). Diagnosis of rotavirus infection in neonatal calves by ribose nucleic acid polyacrylamide electrophoresis (RNA-PAGE) and double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA). Ruminant Science 1(1):15-18.
Kapadiya DK, Joshi DV, Shah NM and Panchasara HH (2012b). Serotyping and in vitro drug sensitivity of Escherichia Coli isolated from neonatal buffalo calves with diarrhoea. Ruminant Science 1(2):141-143.
Kataria N and Kataria AK (2012). Evaluation of health status by serum metabolic profiling in Rathi cows with some disorders. Ruminant Science 1(2):131-135.
Kebede F (2010). Pseudomonas infection in chickens. Journal of Veterinary Medicine and Animal Health 2(4):55-58
Kumar Sanjay, Dadhich Abhilasha, Choudhary Saroj and Mathur M (2015a). Pathological observations of catarrhal enteritis in camel (Camelus dromedarius). Ruminant Science 4(1):55-56.
Kumar S, Jakhar KK, Nehra V and Pal M (2015b). Pathomorphological and microbiological studies in sheep with special emphasis on gastrointestinal tract disorders. Veterinary World 8(8):1015-1020.
Lillie RD (1965). Histopathological Technique and Practical Histochemistry. McGraw Hill Book Co, New York and London
Lounis M, Khadir IH Mokhtari M and Mokhtari Z (2019). Antimicrobial resistance of Escherichia coli isolates from sheep in the Department of Djelfa, Algeria. Agriculture 3(4):111-112.
Luna LG (1968). Manual of Histologic Staining Method of Armed Forces Institute. McGraw Hill Book Co, New York and London
Malaviya Sanjay G, Kalyani IH, Sharma KK, Sakhare PS and Patel DR (2017). Prevalence of toxigenic Escherichia coli (E.Coli) and rotavirus among neonatal calves in south Gurarat region (India) using classical and PCR based methods. Ruminant Science 6(1):139-144.
Manishimwe R, Moncada PM, Musanayire V, Shyaka A, Scott HM and Loneragan G H (2021). Antibiotic-resistant Escherichia coli and salmonella from the feces of food animals in the east province of Rwanda. Animals 11(4):1013.
Mishra AK, Singh DD, Kumar N, Kumarsen G, Paul S and Kumar A (2020). Role of bacterial and parasitic pathogens in occurrence of neonatal diarrhoea in goat-kids. Indian Journal of Animal Research 10(3):389-395
Nayak TC, Singh AP, Savita, Yadav R, Gupta SR and Kachhawa JP (2019). Studies on prevalence and risk factors associated with colibacillosis in cattle calves in Bikaner. Ruminant Science 8(1):89-92.
Osek J (2001). Multiple polymerase chain reaction assay for identification of enterotoxigenic Escherichia coli strains. Journal of Veterinary Diagnostic Investigation 13(4):308-311.
Pachaury Ruchi and Kataria AK (2012). Serogrouping and colicin-related properties of Escherichia coli isolated from neonate diarrhoeic ruminants. Ruminant Science 1(1):9-13.
Padua SB, Debora PM, Fernanda AS, Fabiana P, Mauricio LM and Marcia MI (2014). Isolation, characterization and pathology of Citrobacter freundii infection in native Brazilian catfish Pseudoplatystoma. Brazil Journal of Veterinary Pathology 7(3):151-157.
Poonia Rachna, Mathur Manisha, Dhadhich Hemant, Renu, Kumawat Aditi, Marwaha Sumnil and Rani Sunita (2021). Occurrence and pathology of intestinal tract affections in buffaloes. Ruminant Science 10(1):169-172.
Saleim RS, Wafaa AFE and Nareman AR (2004). Bacteriological and immunological studies on Escherichia coli isolates recovered from diarrhoeic and contact apparently healthy sheep with histopathological changes encountered. Veterinary Medical Journal-Giza 52(2):245-248.
Sayeed A, Khan R, Alam M, Haq F and Hassan J (2010). Isolation and molecular characterization of Escherichia coli from goat of apparently healthy and clinical cases. Bangladesh Journal of Microbiology 27:14-17
Shabana I and Bouqellah N (2017). Investigation of viral and bacterial enteropathogens of diarrhoeic sheep and goat in Medina, Saudi Arabia. Tropical Biomedicine 34(4):1-12.
Sharma SK, Joshi Monika and Tanwar RK (2017). Evaluation of various therapeutic regimens for the treatment of calf diarrhoea. Ruminant Science 6(2):383-388.
Shukla S and Mishra P (2015). Pseudomonas aeruginosa infection in broiler chicks in Jabalpur. International Journal of Extensive Research 6:3-30.
Singh DD, Pawaiya RVS, Gururaj K, Gangwar NK, Mishra AK, Singh R, Andani D and Kumar A (2018). Detection of Clostridium Perfringes toxin types, enteropathogenic E. coli, rota and corona viruses in the intestine of neonatal goat kids by molecular techniques. Indian Journal of Animal Sciences 88(6):655-661
Singh S, Chandratre GA, Dhaka AP and Narang G (2020). Pathomorphological alteration in gastrointestinal tract of sheep affected with Haemonchus conturtus. Haryana Veterinary 59:107-108.
Tanwar Mahendra, Gahlot TK, Palecha Sakar and Bishnoi P (2016). Diagnosis of traumatic reticuloperitonitis in bovines using computed radiography. Ruminant Science 5(2):301-305.
Tiwari AN Jakhar KK, Sharma V and Jangir BL (2018a). Pathomorphological studies in goats effected with gastrointestinal tract disorders. Pharma Innovation Journal 7(3):157-160
Tiwari AN, Jakhar KK and Sharma V (2018b). Pathomorphological study in gastrointestinal tract disorders of sheep. International Journal of Pure and Applied Bioscience 6(2):122-127.
Ye S, Xu S, Haung Y, Zhang L, Wong Z, Jiang W, Ye SZ, Xu SL Haung YJ, Zhang LM, Wong ZJ and Jiang WM (1995). Investigation of Proteus infections in chickens. Chinese Journal of Veterinary Science and Technology’s 25:14-15.
Zain-Eldin, MM, Ghanem, MM, Abd El-Raof, YM and El-Attar HM (2013). Clinical, haematobiochemical and electrocardiographic changes of diarrheic sheep. Benha Veterinary Medical Journal 24:329-342.