12-Title: Molecular identification of epsilon gene and pathomorphology of intestine in sheep with reference to Clostridium perfringens type D
Authors: Renu, H Dadhich, M Mathur, PK Boyal and Subhita Kumari
Source: Ruminant Science (2022)-11(1):53-56.
How to cite this manuscript: Renu, Dadhich H, Mathur M, Boyal PK and Kumari Subhita (2022). Molecular identification of epsilon gene and pathomorphology of intestine in sheep with reference to Clostridium perfringens type D. Ruminant Science 11(1):53-56.
Abstract
The aim of this study was to determine molecular identification of epsilon toxin secreted by Clostridium perfringens type D from intestine of sheep that died due to enterotoxemia and pathological changes observed in intestine. Necropsy was performed on 362 sheep irrespective of age, sex and breeds. Among these, 123 sheep suspected for enterotoxemia were subjected detection of Etx toxin gene of Clostridium perfringens type D from intestinal contents by using PCR. PCR results of 66 sheep samples found positive for Clostridium perfringens type D. PCR positive sheep intestinal tissues were further processed for histopathological studies. Grossly, affected intestines were congested, hemorrhagic and filled with gas. Microscopically, intestine showed goblet cells hyperplasia, edema, haemorrhages, blunted and fused villi, mononuclear cells and polymorphonuclear cells infiltration. In conclusion, Clostridium perfringens type D associated enterotoxemia is a fatal enteric disease which can be identified by histopathological alteration and molecular identification.
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