13-Title: Effect of curcumin as a semen additive on seminal attributes and biochemical parameters of Kankrej bull spermatozoa
Authors: KF Chaudhary, BN Suthar, HC Nakhashi and SK Mohapatra
Source: Ruminant Science (2022)-11(1):57-62.
How to cite this manuscript: Chaudhary KF, Suthar BN, Nakhashi HC and Mohapatra SK (2022). Effect of curcumin as a semen additive on seminal attributes and biochemical parameters of Kankrej bull spermatozoa. Ruminant Science 11(1):57-62.
Total three Kankrej bulls were used to collect 30 semen ejaculates (10 ejaculates from each bull) to determine the effect of different concentrations of curcumin (CUR) as a semen additive in Tris-egg yolk-based semen extender for cryopreservation. Each ejaculate was divided into four equal aliquots and diluted in a Tris-based extender containing curcumin (Control (G-I), 25 µM (G-II), 50 µM (G-III) and 75 µM (G-IV) which were cooled to 5 ºC and frozen in 0.25 ml French straws using programmable bio freezer. Each group was evaluated for initial motility, sperm viability, sperm abnormality, acrosome integrity, Hypoosmotic swelling test, seminal plasma enzymes (ALT and AST) and oxidative stress markers (SOD and LPO). Based on the findings of seminal characteristics, the leakage of AST and ALT enzymes and the oxidative stress markers (SOD activity and LPO levels) at post-equilibrated and post-thawed stages of semen cryopreservation, it was concluded that the addition of 25 µM curcumin in TFYG extender is more suitable to protect the Kankrej bull spermatozoa in comparison to 50 µM and 75 µM curcumin.
Bansal AK and Bilaspuri GS (2011). Impacts of oxidative stress and antioxidants on semen functions. Veterinary Medicine International 2011.
Bilodeau JF, Chatterjee S, Sirard MA and Gagnon C (2000). Levels of antioxidant defenses are decreased in bovine spermatozoa after a cycle of freezing and thawing. Molecular Reproduction and Development: Incorporating Gamete Research 55(3):282-288.
Bucak MN, Baspinar N, Tuncer PB, Coyan K, Sarýozkan S, Akalin PP and Kucukgunay S (2012). Effects of curcumin and dithioerythritol on frozen thawed bovine semen. Andrologia 44:102-109.
Bucak MN, Sariozkan S, Tuncer PB, Sakin F, Ateþþahi A, Kulaksýz R and Cevik M (2010). The effect of antioxidants on post-thawed Angora goat (Capra hircus ancryrensis) sperm parameters, lipid peroxidation and antioxidant activities. Small Ruminant Research 89 (1):24-30.
Buege JA and Aust SD (1978). Microsomal lipid peroxidation. Methods in Enzymology 52: 302-310.
Chanapiwat P and Kaeoket K (2015). The effect of Curcuma longa extracted (curcumin) on the quality of cryopreserved boar semen. Animal Science Journal 86(9):863-868.
Esterbauer H and Cheeseman KH (1990). Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods in Enzymology 186:407-21.
Esteves SC, Sharma RK, Thomas AJ Jr and Agarwal A (2007). Evaluation of acrosomal status and sperm viability in fresh and cryopreserved specimens by the use of fluorescent peanut agglutinin lectin in conjunction with hypo-osmotic swelling test. International Brazilian Journal of Urology 33:364-374.
Ismail AA, Abdel-Khalek AKE, Khalil WA, Yousif AI, Saadeldin IM, Abomughaid MM and El-Harairy MA (2020). Effects of mint, thyme, and curcumin extract nanoformulations on the sperm quality, apoptosis, chromatin decondensation, enzyme activity, and oxidative status of cryopreserved goat semen. Cryobiology 97:144-152.
Jalili C, Khani F, Salahshoor MR and Roshankhah SH (2014). Protective effect of curcumin against nicotine-induced damage on reproductive parameters in male mice. International Journal of Morphology 32(3):844-849.
Jaruga E, Salvioli S, Dobrucki J, Chrul S, Bandorowicz-Pikua J, Sikora E, Franceschi C, Cossarizza A and Bartosz G (1998). Apoptosis-like, reversible changes in plasma membrane asymmetry and permeability and transient modifications in mitochondrial membrane potential induced by curcumin in rat thymocytes. FEBS letters 433(3):287-293.
Kapadiya PS, Nakhashi HC, Chauhan PM, Sutaria TV and Suthar BN (2018). Efficacy of melatonin as an antioxidant for cryopreservation of Kankrej bull semen. Ruminant Science 7(1):89-92.
Kaur Navjot, Sharma Manjinder, Lonare MK and Singh Digvijay (2020). Cytoprotective potential of curcumin and resveratrol during freeze-thaw cycle of buffalo bone marrow derived mesenchymal stem cells. Ruminant Science 9(2):215-222.
Kutty IC, Joseph M and Iyer CPN (1996). A simplified nigrosine-eosin-Giemsa staining technique to distinguish acrosome damaged live and dead spermatozoa. Indian Journal of Animal Research 17(2):122-123.
Mustapha AR, Beigh YA, Abhishek K, Rahul K, Omer D, Kumar A, Prasad JK, Bhure S, Srivastava N and Ghosh SK (2017). Effects of partial deoxygenation of extender on plasma membrane integrity and enzyme activity in frozen-thawed crossbred bull semen. Ruminant Science 6(2):327-331.
Naz RK (2011). Can curcumin provide an ideal contraceptive? Molecular Reproduction and Development 78 (2):116-123.
Omur AD and Coyan K (2016). Protective effects of the antioxidants curcumin, ellagic acid and methionine on motility, mitochondrial transmembrane potential, plasma membrane and acrosome integrity in freeze-thawed Merino ram sperm. Veterinary Medicine 61(1):10-16.
Park EJ, Jeon CH, Ko G, Kim J and Sohn DH (2000) Protective effect of curcumin in rat liver injury induced by carbon tetrachloride. Journal of Pharmacy and Pharmacology 52(4):437-40.
Patel BR and Siddiquee GM (2013). Effect of semen additives on leakage of certain enzymes during cryopreservation of Kankrej semen. Ruminant Science 2(1):63-66.
Quintero-Moreno A, Rubio-Guillen J, Gonzalez-Villalobos D, Gutierrez JC, Madrid-Bury N and Lopez-Brea JJG (2011). Identification of cryo-damage on plasma membrane integrity in bull spermatozoa and its relationship with field fertility. Revista Científica 21(5):403-407.
Rao CV, Desai D, Rivenson A, Simi B, Amin S and Reddy BS (1995). Chemoprevention of colon carcinogenesis by phenylethyl-3- methylcaffeate. Cancer Research 55(11):2310-5.
Ruby J, Kuttan G, Babu KD, Rajashekharan KN and Kuttan R (1995). Antitumor and oxidant activity of natural curcuminoids. Cancer Letters 94:79-83.
Sahrawat Alok K, Saxena Atul and Mishra Sanjay K (2013). Cryopreservation of Bhadawari buffalo bull spermatozoa in tris and citrate based extenders. Ruminant Science 2(1):67-70.
Sayed MM and El-Kordy EA (2014). The protective effect of curcumin on paracetamol-induced liver damage in adult male rabbits: biochemical and histological studies. Egyptian Journal of Histology 37(4):629-639.
Shah SAH, Andrabi SMH and Qureshi IZ (2016). Freezability of water buffalo bull (Bubalus bubalis) spermatozoa is improved with the addition of curcumin (diferuoyl methane) in semen extender. Andrologia 49(8):e12713.
Shukla MK, Misra AK and Gupta HP (2012). Caffeine as semen additive to improve Murrah buffalo (Bubalus bubalis) semen cryopreservation. Ruminant Science 1(2):121-124.
Singh Vipin, Kumar Anuj, Yadava CL, Patel Akhil, Yadav Brijesh, Panday Vijay and Saxena Atul (2018). Effect of season on certain seminal plasma enzymes profile at pre-freeze and post thaw period in the Haryana bull. Ruminant Science 7(2):279-282.
Snedecor GW and Cochran WG (1994). Statistical Methods. 8th Edn, Affiliated East-West Press, New Delhi, India.
Sreejayan and Rao MN (1994). Curcuminoids as potent inhibitors of lipid peroxidation. Journal of Pharmacy and Pharmacology 46(12):1013-1016.
Tvrda E, Greifova H, Mackovich A, Hashim F and Lukac N (2018). Curcumin offers antioxidant protection to cryopreserved bovine semen. Czech Journal of Animal Science 63(7):247-255.
Tvrda E, Tusimova E, Kovacik A, Paal D, Greifova H, Abdramanov A and Lukac N (2016). Curcumin has protective and antioxidant properties on bull spermatozoa subjected to induced oxidative stress. Animal Reproduction Science 172:10-20.
Virmani Meenakshi, Malik Rakesh Kumar, Singh Mahavir, Patil CS, Singh Pradeep and Vijayalakshmy K (2020). Enrichment of semen extender with Syzygium aromaticum extract: It’s impact on bull sperm preservation as an alternative to antibiotics. Ruminant Science 9(1):63-68.
Wang AW, Zhang H, Ikemoto I, Anderson DJ and Loughlin KR (1997). Reactive oxygen species generation by seminal cells during cryopreservation. Urology 49(6):921-925.
Yadava Chhote Lal, Saxena Atul, Yadav Brijesh, Singh Vipin, Reddy A Vidhya Sagar, Patel Akhil, Kumar Anuj and Yadav Sarvajeet (2018). Effect of sericin supplementation on the semen quality of cryopreserved hariana bull semen. Ruminant Science 7(1):93-96.