13-Title: Prevalence and clinico-pathology of bovine respiratory syncytial virus infection in dairy calves

13-Title: Prevalence and clinico-pathology of bovine respiratory syncytial virus infection in dairy calves

Authors: Sameeksha Koundal, Vipan Kumar Gupta, Rajendra Damu Patil and Pooja Thapa

Source: Ruminant Science (2021)-10(2):313-316.

How to cite this manuscript: Koundal Sameeksha, Gupta Vipan Kumar, Patil Rajendra Damu and Thapa Pooja (2021). Prevalence and clinico-pathology of bovine respiratory syncytial virus infection in dairy calves. Ruminant Science 10(2):313-316.


The present research work was performed to determine the prevalence and clinical pathology of bovine respiratory syncytial virus (BRSV) infection in dairy calves. A total of 233 nasal samples including swabs and tissue, were collected from apparently healthy, clinically sick animals and during necropsy of up to one year calves of Himachal Pradesh and Haryana. The samples were examined for BRSV infection by clinico-pathological parameters and molecular detection by RT-PCR assay. Clinical cases of BRSV revealed pyrexia, serous to mucopurulent nasal discharge, serous ocular discharge, violent coughing and abdominal breathing. Necropsy examination showed hyperemic nasal turbinates with or without mucopurulent exudation. Cytology of nasal turbinates revealed clusters of degenerated mucosal epithelium and/or inflammatory cells. Microscopically, nasal turbinates revealed congestion, degenerated and sloughed lining epithelium with variable loss of cilia and infiltration of mononuclear cells in the mucosa. Out of total 233 samples, 145 nasal swabs and 41 nasal turbinate tissues were subjected for BRSV glycoprotein gene (246bp) detection using reverse transcriptase-polymerase chain reaction (RT-PCR) and showed an overall prevalence of 43.64% in dairy calves. It was concluded that BRSV is prevalent in dairy calves and this is the first report of detection of BRSV from nasal samples (swabs and tissues) by RT-PCR from Himachal Pradesh and Haryana in India. Combined clinico-pathological findings and molecular detection were found to be useful in the early and confirmatory diagnosis of BRSV infection in dairy calves.


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