17-Title: Protective effect of quail egg yolk on sperm characteristics of Barbari buck semen during short-term storage at 4 ºC

17-Title: Protective effect of quail egg yolk on sperm characteristics of Barbari buck semen during short-term storage at 4 ºC

Authors: Mukul Anand, Sarvajeet Yadav, Brijesh Yadav, Rahul Dhariya and Deependra Chaudhary

Source: Ruminant Science (2023)-12(1):95-98.

How to cite this manuscript: Anand Mukul, Yadav Sarvajeet, Yadav Brijesh, Dhariya Rahul and Chaudhary Deependra (2023). Protective effect of quail egg yolk on sperm characteristics of Barbari buck semen during short-term storage at 4 ºC. Ruminant Science 12(1):95-98.


In this experiment, different concentrations of quail egg yolk in semen extender of buck were evaluated for their capacity to protect sperm from cold damage during its short-term storage at 4 ºC. Four Barbari bucks maintained at the goat unit were selected for the experiment. Twelve ejaculates were collected from each buck, with a collection frequency of 2 ejaculates per week. Ejaculates of all bucks were collected on the same day and later evaluated and pooled. Each pooled sample was divided in 5 equal aliquots and diluted with a Tris-egg yolk-glycerol-based extender. Sample supplemented with 3% hen egg yolk in the extender was marked as control (C) while the samples diluted with variable concentrations of quail egg yolk in the semen extender were marked as treatment groups’ viz. T1 (2.5%), T2 (10%), T3 (15%) and T4 (20%). Diluted semen was extended to reach a final concentration of 200 x 106 spermatozoa/ml. The diluted sample was stored at 4°C for 48 hours and later evaluated for viability, motility, acrosomal integrity, hypo-osmotic swelling test, path velocity and kinematic characters. Significantly (p<0.01) higher values of per cent live sperm were observed in T2 and T3 while sperm exhibiting progressive motion, per cent uncapacitated sperm, HOST positive sperm and consistency of sperm membrane were significantly (p<0.01) higher in T2 as compared to other groups evaluated during the experiment. Evaluation of motion characteristics exhibited significantly (p<0.01) higher values for curvilinear velocity (VCL, µm / sec), Beat cross frequency (BCF, hz), amplitude of lateral head displacement (ALH, µm) in T2 and T3 while other kinematic characters average path velocity (VAP, µm/sec), straight-line velocity (VSL, µm/sec), Linearity (LIN, %) and Straightness (STR, %) exhibited by sperm were significantly (p<0.01) higher in T3 as compared to other treatments and control. It may be concluded that 10% quail egg yolk in extender has a better capacity to protect sperm against cold damage and can be an alternate to hen egg yolk for short-term storage of buck semen at 4 °C.


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