17-Title: Sero-epidemiology of brucella organisms in cattle, buffalo and human
Authors: Ankita N Modi, HC Chauhan, KK Sharma, SS Patel, AC Patel, MD Shrimali, SK Mohapatra and BS Chandel
Source: Ruminant Science (2023)-12(2):271-278.

Abstract

How to cite this manuscript: Modi Ankita N, Chauhan HC, Sharma KK, Patel SS, Patel AC, Shrimali MD, Mohapatra SK and Chandel BS (2023). Sero-epidemiology of brucella organisms in cattle, buffalo and human. Ruminant Science 12(2):271-278.
Abstract
Brucellosis is recognized as an important occupation zoonotic disease for high-risk group individuals including farmers, veterinarians, and livestock owners. Therefore, the disease surveillance is required in animals as well as humans. A sero-surveillance study was conducted in cattle & buffaloes (635 sera) and humans (300 sera) by using different serological tests and agreement between tests were analysed. In animals, 127/635 (20.00%), 71/635 (11.18%), and 84/635 (13.23%) samples were detected positive by Rose Bengal plate test (RBPT), lateral flow assay (LFA) and i-ELISA, respectively. In cattle, 79/390 (20.26%), 59/390 (15.13%) and 65/390 (16.67%) sera were found positive by RBPT, LFA and i-ELISA, respectively. Whereas in buffaloes (N=245), 48 (19.59%), 12 (4.90%) and 19 (7.76%) samples were found positive by RBPT, LFA and i-ELISA, respectively. Upon i-ELISA, the overall sensitivity and specificity of RBPT and LFA were found to be 88.10% & 90.38%, and 84.52% & 100%, respectively. 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