18-Title: Amplification of FecB (BOOROOLA) gene of BMPR1B in Garole sheep through LAMP technique
Authors: MK Agarwal, NS Rathore, AK Pandey, RC Sharma and B Gupta
Source: Ruminant Science (2022)-11(2):343-346.
How to cite this manuscript: Agarwal MK, Rathore NS, Pandey AK, Sharma RC and Gupta B (2022). Amplification of FecB (BOOROOLA) gene of BMPR1B in Garole sheep through LAMP technique. Ruminant Science 11(2):343-346.
Loop Mediated Isothermal Amplification (LAMP) of DNA is one of the promising isothermal techniques rapidly gaining popularity among researchers. The LAMP technique requires Bst DNA polymerase and a set of 4-6 primers recognizing 6-8 regions of target DNA. The end product comprises 10-30 µg of dumb-bell shaped DNA which can be easily visualized through naked eyes or using a dye. In this report, the assay is extended for genotyping of A/G746 SNP in FecB or Booroola gene situated on chromosome no. 6 of sheep. The developed allele-specific LAMP (AS-LAMP) based colorimetric test finds utility in genotyping sheep breeds with G at the 746th position of mutant-type multiparous sheep and A at the same position in wild-type uniparous sheep breeds. The test requires 61.5 °C and delivers results in merely 31 min. The reaction mixture turns blue colour for mutant-type animals, while remains violet for wild-type animals. The Polymerase Chain Reaction (PCR) amplification of DNA suffers from various drawbacks like the requirement of laboratory and expertise to perform.
Artik Y, Cosgun AB, Cesur NP, Hizel N, Uyar Y, Sur H and Ayan A (2022). Comparison of COVID-19 laboratory diagnosis by commercial kits: Effectively of RT-PCR to the RT-LAMP. Journal of Medical Virology 94(5):1998-2007.
Badolo A, Bando H, Traore A, Ko-ketsu M, Guelbeogo WM, Kanuka H, Ranson H, Sagnon NF and Fufumoto S (2015). Detection of G119S ace-1 R mutation in field-collected Anopheles gambiae mosquitoes using allele-specific loop-mediated isothermal amplification (AS-LAMP) method. Malaria Journal 14:477.
Badolo A, Okado K, Guelbeogo WM, Aonuma H, Bando H, Fukumoto S, Sagnon NF and Kanuka H (2012). Development of an allele-specific, loop-mediated isothermal amplification method (AS-LAMP) to detect the L1014F kdr-w mutation in Anopheles gambiae sl. Malaria Journal 11:227.
Carlos FF, Veigas B, Matias AS, Doria G, Flores O and Baptista PV (2017). Allele specific LAMP-gold nanoparticle for characterization of single nucleotide polymorphisms. Biotechnology of Reproduction 16:21-25.
Duan Y, Zhang X, Ge C, Wang Y, Cao J, Jia, X, Wang J and Zhou M (2014). Development and application of loop-mediated isothermal amplification for detection of the F167Y mutation of carbendazim-resistant isolates in Fusarium graminearum. Scientific Reports 4:7094.
Fischbach J, Xander NC, Frohme M and Glokler JF (2015). Shining a light on LAMP assays’ A comparison of LAMP visualization methods including the novel use of berberine. Biotechniques 58(4):189-194.
Jang WS, Lim DH, Yoon J, Kim A, Lim M, Nam J, Yanagihara R, Ryu SW, Jung, BK, Ryoo H and Lim CS (2021). Development of a multiplex Loop-Mediated Isothermal Amplification (LAMP) assay for on-site diagnosis of SARSCoV-2. PLoS One 16(3):e0248042.
Kaymaz SV, Ergenc AF, Aytekin AO, Lucas SJ and Elitas M (2021). A low cost, portable and practical LAMP device for point of diagnosis in the field. Biotechnology and Bioengineering 119(3):994-1003.
Minnucci G, Amicarelli G, Salmoiraghi S, Spinelli O, Montalvo MLG, Giussani U, Adlerstein D and Rambaldi A (2012). A novel, highly sensitive and rapid allele-specific loop-mediated amplification assay for the detection of the JAK2V617F mutation in chronic myeloproliferative neoplasms. Hematologica 97(9):1394-1400.
Montgomery GW, McNatty KP and Davis GH (1992). Physiology and molecular genetics of mutations that increase ovulation rate in sheep. Endocrine Reviews 13(2):309-328.
Mullis K,Faloona F, Scharf S, Saiki R, Horn G and Erlich H (1986). Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harbor Symp. Quantitative Biology 51:263-273.
Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N and Hase T (2000). Loop-mediated isothermal amplification of DNA. Nucleic Acids Research 28(12):e63.
Piper LR, Bindon BM and Davis GH (1985). Single gene inheritance of the high litter size of the Booroola Merino. In: Genetics of Reproduction in Sheep. Eds: RB Land and DW Robinson, Butterworths. London, UK, pp 115-125.
Wilson T, Wu XY, Juengel JL, Ross IK, Lumsden JM, Lord EA, Dodds KG, Walling GA, McEvan JC, O’ Connel AR, McNatty KP and Montgomery GW (2001). Highly prolific Booroola sheep have a mutation in the intracellular kinase domain of bone morphogenetic protein IB receptor (ALK-6) that is expressed in both oocytes and granulosa cells. Biology of Reproduction 64(4):1225-1235.