18-Title: Amplification of FecB (BOOROOLA) gene of BMPR1B in Garole sheep through LAMP technique
Authors: MK Agarwal, NS Rathore, AK Pandey, RC Sharma and B Gupta
Source: Ruminant Science (2022)-11(2):343-346.
How to cite this manuscript: Agarwal MK, Rathore NS, Pandey AK, Sharma RC and Gupta B (2022). Amplification of FecB (BOOROOLA) gene of BMPR1B in Garole sheep through LAMP technique. Ruminant Science 11(2):343-346.
Abstract
Loop Mediated Isothermal Amplification (LAMP) of DNA is one of the promising isothermal techniques rapidly gaining popularity among researchers. The LAMP technique requires Bst DNA polymerase and a set of 4-6 primers recognizing 6-8 regions of target DNA. The end product comprises 10-30 µg of dumb-bell shaped DNA which can be easily visualized through naked eyes or using a dye. In this report, the assay is extended for genotyping of A/G746 SNP in FecB or Booroola gene situated on chromosome no. 6 of sheep. The developed allele-specific LAMP (AS-LAMP) based colorimetric test finds utility in genotyping sheep breeds with G at the 746th position of mutant-type multiparous sheep and A at the same position in wild-type uniparous sheep breeds. The test requires 61.5 °C and delivers results in merely 31 min. The reaction mixture turns blue colour for mutant-type animals, while remains violet for wild-type animals. The Polymerase Chain Reaction (PCR) amplification of DNA suffers from various drawbacks like the requirement of laboratory and expertise to perform.
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