2-Title: Establishment of primary culture of mammary epithelial cell in Bakarwali goat of Jammu region
Authors: Vaishali Sharma, Gurjeet Kour, Jafrin Ara Ahmed, Nawab Nashiruddullah, Asma Khan, Dipanjali Konwar and Biswajit Brahma
Source: Ruminant Science (2022)-11(2):261-265.
How to cite this manuscript: Sharma Vaishali, t KourGurjee, Ahmed Jafrin Ara, Nashiruddullah Nawab, Khan Asma, Konwar Dipanjali and Brahma Biswajit (2022). Establishment of primary culture of mammary epithelial cell in Bakarwali goat of Jammu region. Ruminant Science 11(2):261-265.
The present study was conducted to establish the primary culture of mammary epithelial cells in the Bakarwali goat of the Jammu region. The luminal epithelial cells of a Bakarwali goat’s mammary tissues were extracted by mincing and grown in the presence of complete media (DMEM F-12, FBS, ITS and EGF). Differential digestion, selective trypsinization, and continuous passaging were used to isolate the cells. By observing the doubling time of epithelial cells, growth properties were evaluated. Trypan blue exclusion method was used to determine cell viability and number. Specific markers (Krt18, Csn2 and Vim) were assessed by their mRNA expression analysis. The primary culture of goat mammary epithelial cells exhibited typical cobblestone morphology and growth characteristics. The primary mammary epithelial cell culture would facilitate research in mammary gland development and function.
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