2-Title: Establishment of primary culture of mammary epithelial cell in Bakarwali goat of Jammu region
Authors: Vaishali Sharma, Gurjeet Kour, Jafrin Ara Ahmed, Nawab Nashiruddullah, Asma Khan, Dipanjali Konwar and Biswajit Brahma
Source: Ruminant Science (2022)-11(2):261-265.
How to cite this manuscript: Sharma Vaishali, t KourGurjee, Ahmed Jafrin Ara, Nashiruddullah Nawab, Khan Asma, Konwar Dipanjali and Brahma Biswajit (2022). Establishment of primary culture of mammary epithelial cell in Bakarwali goat of Jammu region. Ruminant Science 11(2):261-265.
Abstract
The present study was conducted to establish the primary culture of mammary epithelial cells in the Bakarwali goat of the Jammu region. The luminal epithelial cells of a Bakarwali goat’s mammary tissues were extracted by mincing and grown in the presence of complete media (DMEM F-12, FBS, ITS and EGF). Differential digestion, selective trypsinization, and continuous passaging were used to isolate the cells. By observing the doubling time of epithelial cells, growth properties were evaluated. Trypan blue exclusion method was used to determine cell viability and number. Specific markers (Krt18, Csn2 and Vim) were assessed by their mRNA expression analysis. The primary culture of goat mammary epithelial cells exhibited typical cobblestone morphology and growth characteristics. The primary mammary epithelial cell culture would facilitate research in mammary gland development and function.
References
Ahn JY, Aoki N, Adachi T, Mizuno Y, Nakamura R and Matsuda T (1995). Isolation and culture of bovine mammary epithelial cells and establishment of gene transfection conditions in the cell. Bioscience, Biotechnology and Biochemistry 59:59-64.
Akers RM (2002). Lactation and the Mammary Gland. Ames, Iowa: Iowa State University Press. p 278.
Anand V, Dogra N, Singh S, Kumar SN, Jena, MK, Malakar D, Dang AK, Mishra BP, Mukhopadhyay TK, Kaushik JK and Mohanty AK (2012). Establishment and characterization of a buffalo (Bubalus bubalis) mammary epithelial cell line. PLoS ONE 7:e40469
Cao YH, J Luo J, Zhang M, Zhao XE and Zhen HL (2008). Morphological observation of mammary epithelial cells of goats in vitro. Chinese Journal of Veterinary Medicine 44:20-21.
Choudhary RK and Choudhary Shanti (2012). Ruminant mammary stem cells: Methods of identification and status. Ruminant Science 1(2):101-108.
Choudhary Ratan K, Pathak Devendra, Deka Dipak and Ramneek (2013). Vimentin identifies myoepithelial cells of buffalo mammary tissue. Ruminant Science 2(2):157-161.
Colomb E, Berthon P, Dussert C, Calvo F and Martin PM (1991). Estradiol and EGF requirements for cell-cycle progression of normal human mammary epithelial cells in culture. International Journal of Cancer 49:932-937.
Fu M, ChenY, Xiong X, Lan D and Li J (2014). Establishment of mammary gland model: Culture in vitro and evaluation of a yak mammary epithelial cell line. PLoS One 9:e113669.
Jedrzejczak M and Szatkowska I (2014). Bovine mammary epithelial cell cultures for the study of mammary gland functions. In Vitro Cellular and Developmental Biology-Animal 50:389-398.
Jernej O, Zorc M and Dovc P (2018). Development of an in vitro goat mammary model: Establishment, Characterization and Applications of Primary Goat Mammary Cell Cultures. Intech Open 69:10-18.
Ouyang WQ and Qian JF (1999). Establishment of goat mammary epithelial cell line. Chinese Journal of Veterinary Medicine 19:561-562.
Pal K and Grover PL (1983). A simple method for the removal of contaminating fibroblasts from cultures of rat mammary epithelial cells. Cell Biology International Reports 7(10):779-83.
Pantschenko AG, Woodcock-Mitchell J, Bushmich SL and Yang TJ (2000). Establishment and characterization of a caprine mammary epithelial cell line (CMEC). In Vitro Cellular and Developmental Biology-Animal 36(1):26-37.
Peng XR, Zheng YM and Zhang Y (2005). Study on the appearance of in vitro-cultured bovine mammary epithelial cells. Journal of Northwest Sci-Tech University of Agriculture and Forestry 33:13-17.
Stampfer MR, Yaswen P and Taylor-Papadimitriou J (2002). Culture of human mammary epithelial cells. In: Culture of the Epithelial Cells. Eds: RI Freshney and MG Freshney, Wiley-Liss Inc, New York, pp 95-135.
Tong HL, Li QZ, Gao XJ and Yin DY (2012). Establishment and characterization of a lactating dairy goat mammary gland epithelial cell line. In Vitro Cellular and Developmental Biology -Animal 48:149-155.
Warri AM, Saarinen NM, Makela SI (2007) Can modulation of mammary gland development by dietary factors support breast cancer prevention? Hormne Research 68:248–260