2-Title: Oxidative stress, immunological and systemic inflammatory changes in lumpy skin disease affected cattle

2-Title: Oxidative stress, immunological and systemic inflammatory changes in lumpy skin disease affected cattle

Authors: Sandeep, Dhawal Kant Yadav, Amit Kumar, JP Kachhawa, Sita Ram Gupta, AP Singh and Rajender Yadav

Source: Ruminant Science (2023)-12(1):9-12.

How to cite this manuscript: Sandeep, Yadav Dhawal Kant, Kumar Amit, Kachhawa JP, Gupta Sita Ram, Singh AP and Yadav Rajender (2023). Oxidative stress, immunological and systemic inflammatory changes in lumpy skin disease affected cattle. Ruminant Science 12(1):9-12.


The present study was conducted to access the alterations in oxidative stress markers, immunological markers, and inflammatory markers in Lumpy skin disease (LSD)-affected cattle. Thirty-two adult cattle irrespective of sex and breed were selected out of which eight animals were observed and inspected as apparently healthy and considered as the control group and the other 24 animals with apparent clinical signs and confirmed positive by PCR were considered as the infected group. Clinical manifestations were observed and blood as well as sera samples were collected from both groups. Results of oxidative stress markers revealed that significant decrease in TAC and GSH-Px, while a significant increase in MDA and CAT in the serum of LSD-affected animals. Serum immunological markers revealed a significant increase in values of IgG and IgM in diseased animals. The results of inflammatory cytokines revealed that significant increase in IL-4, TNF-alpha, and IFN-gamma in affected animals. This study indicates that LSDV infection provokes oxidative stress and systemic inflammation as well as stimulates the host response to viral infection. This study will help in exploring the pathogenesis of LSD and adopting an anti-oxidant, immunomodulatory, and anti-inflammatory remedial strategy for LSD-affected animals.


Agag BI, Mousa S, Hassan HB, Saber MS, El-Deghidy NS and El-Aziz AMA (1992). Clinical, serological and biochemical studies on lumpy skin disease. Journal of Applied Animal Research 1(1):13-23.

Ali H, Ali AA, Atta MS and Cepica A (2012). Common, emerging, vector borne and infrequent abortogenic virus infections of cattle. Transboundary and Emerging Diseases 59(1):11-25.

Boobis AR, Fawthrop DJ and Davies DS (1989). Mechanisms of cell death. Trends in Pharmacological Sciences 10(7):275-280.

Chen N, Bellone CJ, Schriewer J, Owens G, Fredrickson T, Parker S and Buller RML (2011). Poxvirus interleukin-4 expression overcomes inherent resistance and vaccine-induced immunity: Pathogenesis, prophylaxis and antiviral therapy. Virology 409(2):328-337.

El-Bahr SM and El-Deeb WM (2013). Acute phase proteins, lipid profile and pro-inflammatory cytokines in healthy and broncho-pneumonic water buffalo calves. American Journal of Biochemistry and Biotechnology 9(1):34-40.

El-Mandrawy SA and Alam RT (2018). Hematological, biochemical and oxidative stress studies of lumpy skin disease virus infection in cattle. Journal of Applied Animal Research 46(1):1073-1077.

Estes DM, Templeton JW, Hunter DM and Adams LG (1990). Production and use of murine monoclonal antibodies reactive with bovine IgM isotype and IgG subisotypes (IgG1, IgG2a and IgG2b) in assessing immunoglobulin levels in serum of cattle. Veterinary Immunology and Immunopathology 25(1):61-72.

Fay PC, Wijesiriwardana N, Munyanduki H, Sanz-Bernardo B, Lewis I, Haga IR, Moffat K, van Vliet AHM, Hope J, Graham SP and Beard PM (2022). The immune response to lumpy skin disease virus in cattle is influenced by inoculation route. Frontiers in Immunology 13:1051008.

Gari G, Waret-Szkuta A, Grosbois V, Jacquiet P and Roger F (2010). Risk factors associated with observed clinical lumpy skin disease in Ethiopia. Epidemiological Infect 138(11):1657-1666.

Gornall AG (1980). Applied Biochemistry of Clinical Disorders. 2nd Edn, Harper Collins Publishers.

Hanan K, Heba G, Norhan N and Mohamed R (2016). 13th Science Congres, Egyptian Society for Cattle Disease 1-4 Feb, Hurggada, Egypt.

Karki R, Sharma BR, Tuladhar S, Williams E P, Zalduondo L, Samir P and Kanneganti TD (2021). Synergism of TNF-alpha and IFN-gamma triggers inflammatory cell death, tissue damage, and mortality in SARS-CoV-2 infection and cytokine shock syndromes. Cell 184(1):149-168.

Kataria N, Kataria AK, Maan R and Gahlot AK (2010). Evaluation of oxidative stress in brucella infected cows. Journal of Stress Physiology and Biochemistry 6(2):19-25.

Kumar N, Chander Y, Kumar R, Khandelwal N, Riyesh T, Chaudhary K and Tripathi BN (2021). Isolation and characterization of lumpy skin disease virus from cattle in India. PLoS One 16(1): e0241022.

Kumari Rashmi Rekha, Tripathi Manoj Kumar, Kumar Ravi, Kumar Prabhas, Kumar Manish, Kumar Abhay, Sarma Kamal and Kumar Pankaj (2021). Intricacy in cross-bred cattle with presumptive lumpy skin disease and prospective antiviral role of ivermectin. Ruminant Science 10(2):375-382.

Moran TM, Isobe H, Fernandez-Sesma A and Schulman JL (1996). Interleukin- 4 causes delayed virus clearance in influenza virus-infected mice. Journal of Virology 70(8):5230-5235.

Mulatu E and Feyisa A (2018). Lumpy skin disease. Journal of Veterinary Sciences 9(535):1-8.

Nashwa M H, Ahmed S A and Zeinab Y (2017). Molecular, clinico-pathological and sero-diagnosis of LSDV in cattle at Sharkia and Fayoum Governorates. Journal of Virological Sciences 1:1-11.

Neamat-Allah AN (2015). Immunological, hematological, biochemical and histopathological studies on cows naturally infected with lumpy skin disease. Veterinary World 8(9):1131.

Radostits OM, Gay CC, Hinchcliff KW and Constable PD (2007). Veterinary Medicine. 10th Edn, Saunders, Elsevier, Philadelphia, USA. pp 673-748.

Ratnam DV, Ankola DD, Bhardwaj V, Sahana DK and Kumar MR (2006). Role of antioxidants in prophylaxis and therapy: A pharmaceutical perspective. Journal of Controlled  Release 113(3):189-207.

Salib FA and Osman AH (2011). Incidence of lumpy skin disease among Egyptian cattle in Giza Governorate. Egyptian Veterinary World 4(4):162-67.

Sheikh FA, Manas KP, Mahendran K, Babul RP, Khanna S, Singh AK, De UK,  Agrawal RK, Gaur GK and  Dutt T (2023). Hematological and serum biochemical parameters and profiling of cytokine genes in lumpy skin disease in Vrindavani cattle. Biotech 13:66.

Sudhakar SB, Mishra N, Kalaiyarasu S, Jhade SK, Hemadri D, Sood R and Singh VP (2020). Lumpy skin disease (LSD) outbreaks in cattle in Odisha state, India in August 2019: Epidemiological features and molecular studies. Transboundary and Emerging Diseases 67(6):2408-2422.

Snedecor GW and Cochran WG (1994). Statistical Method. 8th Edn, Iowa State College Press, Iowa USA. p 950.

Tageldin MH, Wallace DB, Gerdes GH, Putterill JF, Greyling RR and Phosiwa MN (2014). Lumpy skin disease of cattle: An emerging problem in the Sultanate of Oman. Tropical Animal Health Production 46(1):241-246.

Tuppurainen E, Alexandrov T and Beltrán-Alcrudo DJFAP (2017). Lumpy Skin Disease a Manual for Veterinarians. FAO Animal Production and Health Manual (20).