25-Title: Evaluation of therapeutic potential of Dalbergia sissoo and Punica granatum on clinical cases of calf diarrhoea

25-Title: Evaluation of therapeutic potential of Dalbergia sissoo and Punica granatum on clinical cases of calf diarrhoea

Authors: Himanshu Agrawal, Arvind Kumar Tripathi, Ashish Srivastava, Ambika Sharma, PN Panigrahi, Manu Jaiswal and Shubhasini Dhuriya

Source: Ruminant Science (2021)-10(2):367-374.

How to cite this manuscript: Agrawal Himanshu, Tripathi Arvind Kumar, Srivastava Ashish, Sharma Ambika, Panigrahi PN, Jaiswal Manu and Dhuriya Shubhasini (2021). Evaluation of therapeutic potential of Dalbergia sissoo and Punica granatum on clinical cases of calf diarrhoea. Ruminant Science 10(2):367-374.


Present study was conducted on 24 diarrhoeic calves and 6 healthy calves of Sahiwal breed aged up to one month to evaluate the therapeutic potential of Dalbergia sissoo and Punica granatum. After shed drying of leaves of Dalbergia sissoo and peel of Punica granatum powder was prepared. The diarrhoeic calves were divided in four treatment groups T1, T2, T3 and T4, and one group was the healthy control group (Hc). The powder was supplemented at the dose rate of 50gm bid for five days. In addition to powder Ringer lactate fluid @ of 25 ml/kg IV along with antiinflammatory and multivitamin were administered. The therapeutic efficacy of the above therapeutic regimens was evaluated based on clinical recovery (days of recovery) and improvement in the altered values of the clinical score, haemato-biochemical alterations and electrolyte values towards normalcy (as par to the values in healthy control calves) on day 6th after treatment. Clinical observations and blood sampling were done on the day of occurrence of diarrhoea and on day 6th post-treatment. Blood samples were collected on day 0 and day 6th, from the jugular vein in clean glass tubes for haemato-biochemical alterations before treatment. Complete blood count was done by haematology analyzer. A total of 5 ml blood was collected from each animal in a sterilized glass tube at each of the specified intervals for serum biochemistry. Blood was allowed to coagulate by keeping the tubes in slants and serum was separated by spinning at 3000 rpm for 10 minutes. Therapeutic regimen adopted in the treatment of acute diarrhoeic calves under groups T5 in which Dalbergia sissoo and Punica granatum powder were given was found to be most efficacious as evident by the improvement in clinical score in terms of rehydration, improved faecal consistency, depression score and improved haemato-biochemical alteration.


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