25-Title: Superovulatory response, in vivo embryo production and birth of et calves in Gir cow’ (Bos indicus) at PGIVER, RAJUVAS, Jaipur

25-Title: Superovulatory response, in vivo embryo production and birth of et calves in Gir cow’ (Bos indicus) at PGIVER, RAJUVAS, Jaipur

Authors: Chandra Shekher Sarswat, Navav Singh, Nirmal Jeph, Anand Kumar, Sanjita Sharma and SP Singh

Source: Ruminant Science (2022)-11(1):121-124.

How to cite this manuscript: Sarswat Chandra Shekher, Singh Navav, Jeph Nirmal, Kumar Anand, Sharma Sanjita and Singh SP (2022). Superovulatory response, in vivo embryo production and birth of et calves in Gir cow’ (Bos indicus) at PGIVER, RAJUVAS, Jaipur. Ruminant Science 11(1):121-124.


Donor Gir cows (Bos indicus) in two phases first (n=4) and second (n=3) were subjected to superovulation with 140 micrograms of injection stimufol (pFSH and pLH) divided into morning and evening decreasing doses over 4 days to study the superovulatory response and in vivo embryo production and subsequent birth of ET calves during the two different seasons (Autumn (Ist) and Winter (IInd)) at LFC, PGIVER, RAJUVAS, Jaipur. Duration of estrus and corpus luteum count were used to determine the super ovulatory response of donor Gir cows in both phases. The responded donors in phase-I (04) and phase-II (02) were flushed and embryos were retrieved on day 07 post artificial insemination by non-surgical method and further evaluated for the development stage and quality grade as per IETS standards. The duration of from injection prostaglandin (along with 3rd 3rd day of injection of stimufol) was recorded as 24-48 hours and 48-72 hours in phase I and II, respectively. The total corpus luteum count of all animals was higher in phase I (36) as compared to phase II (24). A mean number of 7.25 and 9.5 embryos were retrieved from donor Gir cows during phase –I and phase –II, respectively. The total number of transferable embryos produced is 29 and 19 and the number of embryos transferred in each phase is 21 and 15 with average transferable embryos were 5.25±0.85 and 7.5±5.5 in phase –I and phase –II, respectively. Subsequently, one male live ET calf during phase-I and two dead male and two female live ET calves during phase–II were produced from recipients. It was concluded that Gir cows’ response to superovulation and yield of better quality and number of embryos showed the high potential of the breed for in-vivo embryo production.


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