25-Title: Superovulatory response, in vivo embryo production and birth of et calves in Gir cow’ (Bos indicus) at PGIVER, RAJUVAS, Jaipur

Authors: Chandra Shekher Sarswat, Navav Singh, Nirmal Jeph, Anand Kumar, Sanjita Sharma and SP Singh

Source: Ruminant Science (2022)-11(1):121-124.

How to cite this manuscript: Sarswat Chandra Shekher, Singh Navav, Jeph Nirmal, Kumar Anand, Sharma Sanjita and Singh SP (2022). Superovulatory response, in vivo embryo production and birth of et calves in Gir cow’ (Bos indicus) at PGIVER, RAJUVAS, Jaipur. Ruminant Science 11(1):121-124.

Abstract

Donor Gir cows (Bos indicus) in two phases first (n=4) and second (n=3) were subjected to superovulation with 140 micrograms of injection stimufol (pFSH and pLH) divided into morning and evening decreasing doses over 4 days to study the superovulatory response and in vivo embryo production and subsequent birth of ET calves during the two different seasons (Autumn (Ist) and Winter (IInd)) at LFC, PGIVER, RAJUVAS, Jaipur. Duration of estrus and corpus luteum count were used to determine the super ovulatory response of donor Gir cows in both phases. The responded donors in phase-I (04) and phase-II (02) were flushed and embryos were retrieved on day 07 post artificial insemination by non-surgical method and further evaluated for the development stage and quality grade as per IETS standards. The duration of from injection prostaglandin (along with 3rd 3rd day of injection of stimufol) was recorded as 24-48 hours and 48-72 hours in phase I and II, respectively. The total corpus luteum count of all animals was higher in phase I (36) as compared to phase II (24). A mean number of 7.25 and 9.5 embryos were retrieved from donor Gir cows during phase –I and phase –II, respectively. The total number of transferable embryos produced is 29 and 19 and the number of embryos transferred in each phase is 21 and 15 with average transferable embryos were 5.25±0.85 and 7.5±5.5 in phase –I and phase –II, respectively. Subsequently, one male live ET calf during phase-I and two dead male and two female live ET calves during phase–II were produced from recipients. It was concluded that Gir cows’ response to superovulation and yield of better quality and number of embryos showed the high potential of the breed for in-vivo embryo production.

References

Agrawal Jitendra Kumar, Saxena Atul, Kharche SD, Pathak Juhi, Sikarwar AKS, Patel Akhil, Goel AK and Jindal SK (2016a). A study on parthenogenetic activation and embryo production from in vitro matured caprine oocytes. Ruminant Science 5(1):21-24.

Agrawal JK, Saxena A, Kharche SD, Kumar A, Yadav S and Pandey RP (2016b). Effects of different oxygen concentrations on developmental potential of goat tetraploid embryos. Ruminant Science 5(2):239-242.

Agrawal JK, Kharche SD, Saxena A, Yadav S and Panday RP (2017). A study on embryo production through in-vitro fertization of caprine oocytes. Ruminant Science 6(2):333-336.

Alvarez RH (1994). Recentes progressos na superovulação dos bovinos. Zootecnia 32:3-10.

Baruselli PS, De Sa Filho MF, Martins CM, Nasser LF, Nogueira MF, Barros CM and Bo GA (2006). Super ovulation and embryo transfer in Bos indicus cattle. Theriogenology 65:77-88.

Breuel KF, Baker RD, Butcher RL, Townsend EC, Inskeep EK, Dailey RA and Lerner SP (1991). Effect of breed, age of donor and dosage of follicle stimulating hormone on the superovulatory response of beef cows. Theriogenology 36:241-255.

Contreras David, Galina Carlos, Avila JG, Aspron Marco and Moreno-Mendoza N (2008). A system to evaluate the quality of frozen embryos through short-term culture. Animal Reproduction Science 106:369-79.

Grizelj J, Vince S, Samardzija M, Gonzalez de Bulnes A, Dovenski T, Turmalaj L and Ževrnja B (2013). Use of ultrasonography to detect ovarian response in goats submitted to multiple ovulation and embryo transfer program. Veterinarski Arhiv 83:125-134.

Kafi M and McGowan MR (1997). Factors associated with variation in the superovulatory response of cattle. Animal Reproduction Science 48(2-4):137-57.

Lerner SP, Thayne WV, Baker RD, Henschen T, Meredith S, Inskeep EK, Dailey RA, Lewis PE and Butcher RL (1986). Age, dose of FSH and other factors affecting super ovulation in Holstein cows. Journal of Animal Science 63(1):176-83.

Monniaux D, Chupin D and Saumande J (1983). Superovulatory responses of cattle. Theriogenology 19:55-82.

Puri Gopal and Bag Sadhan (2014). Generation of parthenogenetic embryos by ethanol activation in buffalo. Ruminant Science 3(1):5-7.

Quadri SA, Shukla SN, Singh AP, Kumar D and Sarkhel BC (2021). Effect of leptin and insulin like growth factor-I on in vitro maturation and subsequent development of goat oocytes. Ruminant Science 10(2):337-342.

Robertson I and Nelson RE (1998). Certification and identification of the embryo. In: Manual of the International Embryo Transfer Society (IETS).  Eds: Stringfellow DA, Seidel SM, Savoy, IL, pp 103-134.

Saraswat CS, Singh SP, Bhalodia S and Singh RK (2018a). Production of first Tharparkar ET calves at Livestock Research Station, Beechwal RAJUVAS, Bikaner. 34 annual convention of ISSAR and international symposium organized by AAU, Gujarat during December, 28-30.

Saraswat CS, Singh RK, Sharma S, Singh SP, Bhalodia S and Soni M (2018b). Recovery of Genetically superior embryos from Tharparkar Donor cows under Tharparkar Embryo Transfer Programme at Livestock Research Station, Beechwal. National symposium & XV annual convention of SOCDAB at RJUVAS, Bikaner held on February 8-10, p 121.

Silva JCC, Alvarez RH, Zanenga CA, Pereira GT, Alvarez RH, Zanenga CA and Pereira GT (2009).  Factors affecting embryo production in superovulated Nelore cattle. Animal Reproduction 6(3):440-445.

Singh Vijay, Misra AK, Kumar Suresh and Kumar Vijay (2015). Effect of seasons on in-vitro maturation and cleavage of oocyte and in-vitro development of buffalo embryo. Ruminant Science 4(1):29-31.

Stroud B and Hasler JF (2006). Dissecting why superovulation and embryo transfer usually work on some farms but not on others. Theriogenology 65:65-76.

Suresh A, Shukla MK, Kumar Dharmendra, Shrivastava OP and Verma Neeraj (2020). Efficacy of epididymal spermatozoa for in vitro fertilization in caprine species. Ruminant Science 9(2):383-386.