26-Title: Comparative studies on blood biochemical profile of cyclic, pregnant and infertile cows

26-Title: Comparative studies on blood biochemical profile of cyclic, pregnant and infertile cows

Authors: Pravesh Kumar, Madhumeet Singh, Akshay Sharma, Vijay Kumar, Ravindra Kumar and Pururava Sharma

Source: Ruminant Science (2022)-11(1):125-130.

How to cite this manuscript: Kumar Pravesh, Singh Madhumeet, Sharma Akshay, Kumar Vijay, Kumar Ravindra and Sharma Pururava (2022). Comparative studies on blood biochemical profile of cyclic, pregnant and infertile cows. Ruminant Science 11(1):125-130.


The present study was undertaken in cows (n=830) of different districts of Himachal Pradesh to study the effect of different minerals and biochemical attributes on animal infertility. These profiles of different types of infertile (endometritis, anestrus, silent estrus and cows showing metestrual bleeding) cows were evaluated and compared with normal cyclic and pregnant animals. The collected blood samples were analyzed for calcium, phosphorous, magnesium, copper, zinc, sodium, potassium, ALT, AST, total protein, creatinine and cholesterol. Most of the minerals were found deficits in the blood of anestrus and silent estrus cows when compared to normal cyclic and pregnant animals.


Ahmad I, Lodhi LA, Qureshi ZI and Younis M (2004).  Studies on blood glucose, total proteins, urea and cholesterol levels in cyclic, non-cyclic and endometritic crossbred cows. Pakistan Veterinary Journal 22(4):92-94.

Ahmed ME, Ahmed FO, Frah EAM and Elfaki I (2017). Blood biochemical profile of Sudanese crossbred repeat breeder cows. African Journal of Biotechnology 16(8):366-370.

Bindari YR, Shrestha S, Shrestha N and Gaire TN (2013). Effect of nutrition on reproduction-a review. Advances in Applied Science Research 4(1):421-429.

Chaurasia R, Kushwaha HS, Chaurasia D, Gendley MK and Santra AK (2016). Comparative studies of certain macro minerals during various reproductive states in buffaloes. Buffalo Bulletin 35 (1):33-38.

Chesworth J (1992). Ruminant Nutrition: Book Series. The Tropical; Agriculturist. McMillan Press Ltd. London, UK.

Cromwell GL (1997). Handbook of Copper Compounds and Applications. pp 177-202.

Das S, Bondopadhaya SK, Ghose BB and Dasgupta R (2002). Blood mineral profile of normal cyclic and repeat breeder crossbred cows under rural condition. Indian Journal of Animal Reproduction 23(2):167-169.

Desai SP, Sharma VK, Chauhan PM, Sutaria TV, Suthar BN and Mody SK (2018). Gynaeco-clinical and physical properties of cervical mucus in repeat breeding crossbred cows following E.Coli LPS therapy. Ruminant Science 7(1):77-82.

Dutta A, Baruah B, Sharma BC, Baruah KK and Goswami RN (2001). Serum macro mineral profile in cyclic and anestrus local heifers in lower Brahmaputra valley of Assam. Indian Journal of Animal Resource 35(1):44-46.

Dutta J, Sarma S, Kalita DJ, Magotra Ankit and Hazorika Mousumi (2016). Induction of oestrus in postpartum anoestrus crossbred cows. Ruminant Science 5(1):63-65.

Elrod CC and Butler WR (1993). Reduction of fertility and alteration of uterine pH in heifers fed excess ruminally degradable protein. Journal of Animal Science 71:694-701.

Gandotra VK, Choudhary KR and Sharma RD (1993). Serum biochemical constituents in normal and repeat breeder Rathi cows. Indian Journal of Animal Sciences 52:944-946.

Greene LW, Johnson AB, Paterson JA and Ansotegui RP (1998). Role of trace minerals in cow-calf cycle examined. Feedstuffs 70:34.

Gupta KK, Shukla SN and Shukla MK (2015). Prevalence of anoestrus in postpartum dairy buffaloes of Jabalpur. Ruminant Science 4(2):235-238.

Kalita DJ and Sarmah BC (2006). Mineral profile and serum enzymes activities of normal cycling and repeat breeding cows. Indian Journal of Animal Resources 40(1):49-51.

Kalita DJ, Barua KK, Goswami D, Saikia K and Dutta R (2015). Evaluation of micromineral status and ovarian hormonal profile and their interrelation in normal cyclings and repeat breeding cows. Indian Journal of Animal Research 49(6):798-801.

Kapadiya FM, Dadawala AI, Makwana PP, Bhati NB, Patel TP and Sutariya TV (2020). Biochemical attributes in repeat breeder Mehsana buffaloes. Ruminant Science 9(1):59-61.

Kekan PM and Shirbhate RN (2015). Biochemical status during oestrus cycle in regular and repeat breeding cows. Theriogenology Insight 5(3):213-217.

Marai IF, El-Darawany AA and Nasr AS (1992). Typical repeat breeding and its improvement in buffaloes. Beitrage zur Tropischen Landwirtschaft und Veterinarmedizin 30:305-14.

Merck Veterinary Manual (2005). Serum Biochemical Reference Ranges. 9th Edn, Merck and Co, Inc, USA.

Mimoune N, Messai CR, Khelef D, Salhi O, Azzouz MY and Kaidi R (2017). Reproductive parameters and metabolic profile of repeat breeder cows. Livestock Research for Rural Development 29(8):1-6.

Mishra Pankaj, Rajput DS, Yadav ML and Swami PD (2014). Constraints perceived by tribal livestock owners in Banswara district of Rajasthan. Ruminant Science 3(2):201-203.

Mishra SK (2006). Effect of supplementation of minerals on the reproductive performance of cows in Dhenkanal district of Orissa. PhD thesis submitted to OUAT, Bhubaneshwar, Odisha.

Modi LC, Suthar BN, Sharma VK, Nakhashi HC, Panchasara HH and Modi F (2017). Comparative biochemical profile of blood serum and estrual mucus in normal and repeat breeding Kankrej cow. Indian Journal of Animal Health 56(1):53-58.

Mudgal Vishal, Gupta Vishnu Kumar, Srivastava Shalini and Ganai Ajaz Ahmed (2012). Effect of species variation on level of different trace elements in the serum of anoestrus cattle. Ruminant Science 1(2):1-7.

Mudgal V, Gupta VK, Pankaj PK, Srivastava S and Ganai AA (2014). Effect of copper supplementation on the onset of estrus in anestrous buffalo cows and heifers. Buffalo Bulletin 33(1):1-5.

Pal SK, Mohanty BN, Ray SKH and Mohanty DN (1991). Studies on serum protein, cholesterol and certain enzymes in relation to reproductive status in bovine females. Indian Journal of Animal Research 12:28-29.

Pandey V, Singh AK and Sharma N (2009). Blood biochemical profile in fertile and repeat breeding crossbred cows under field conditions. Veterinary Practitioner 1:45-48.

Pariza KF, Alam J, Islam M R, Hossain MM and Awal MA (2013). Investigation of hematological and biochemical profiles of anestrous zebu cows. Bangladesh Journal of Veterinary Medicine 11(1):57-60.

Parkinson TJ (2009). Infertility in cow. In: Arthur’s Veterinary Reproduction and Obstetrics. Noakes DE, Parkinson TJ and England GCW, 9th Edn, WB Saunders Company, Philadelphia. pp 393-558.

Parmar KS, Mehta VM and Patel JM (1986). Biochemical profile of repeat breeding cattle in relation to different phases of oestrous cycle. Indian Journal of Animal Reproduction 7:31-35.

Patterson HH, Adams DC, Klopfenstein TJ, Clark RT and Teichert B (2003). Supplementation to meet metabolizable protein requirements of primiparous beef heifers: II. Pregnancy and Economics. Journal of Animal Science 81:503-570.

Pradhan R and Nakagoshi N (2008). Reproductive disorder in cattle due to nutritional status. Journal of International Development and Cooperation 14(1):45-66.

Reddy DV (2010). Principles of Animal Nutrition and Feed Technology. 2nd Edn, New Delhi, India: Oxford and IBH Publishing Company Pvt. Ltd.

Sales JNS, Pereira RVV, Bicalho RC and Baruselli PS (2011). Effect of injectable copper, selenium, zinc and manganese on the pregnancy rate of crossbred heifers (Bos indicus × Bos taurus) synchronized for timed embryo transfer. Livestock Science 142(1-3):59-62.

Singaram B and Jacob T (2017). Comparative studies on blood biochemical profile of anestrus and normal cyclic Jersey cross-bred cows. International Journal of Livestock Research 7(3):40-44.

Solanki VL, Sharma VK, Chauhan PM, Prajapati BI, Desai SP and Nakhashi HC (2019). Effect of oyster glycogen on cervical mucus microbiota and conception rate in repeat breeding cross bred cows. Ruminant Science 8(1):49-52.

Ugyen P, Penjor and Dorji R (2016). Effect of serum calcium, magnesium and phosphorous levels in cyclic and postpartum anestrus jersey cross cow. Bhutan Journal of Natural Resources and Development 3(1):1-5.

Yasothai R (2014). Importance of minerals on reproduction in dairy cattle. International Journal of Science, Environment and Technology 3(6):2051-2057.

Zaman MI, Sharma U, Razzaque WAA, Kumar S and Kumar S (2014). Studies on certain blood biochemical constituents in normal and repeat breeding crossbred cows. Indian Journal of Animal Reproduction 35(2):24-26.