27-Title: Prevalence of paratuberculosis in cattle of Jaipur city, Rajasthan

27-Title: Prevalence of paratuberculosis in cattle of Jaipur city, Rajasthan

Authors: Rahul Tiwari, Rashmi Singh, Nirmal K Jeph, DS Meena, Nazeer Mohammad and Sarjna Meena

Source: Ruminant Science (2021)-10(2):383-388.

How to cite this manuscript: Tiwari Rahul, Singh Rashmi, Jeph Nirmal K, Meena DS, Mohammad Nazeer and Meena Sarjna (2021). Prevalence of paratuberculosis in cattle of Jaipur city, Rajasthan. Ruminant Science 10(2):383-388.


The present study was conducted to evaluate the prevalence of paratuberculosis in cattle. A total of 200 serum samples were collected from cattle of a gaushala in Jaipur city and subjected to ELISA and single intradermal tuberculin test. For bovine paratuberculosis, the overall prevalence obtained was 3.5% in cattle. Individual test wise, 5.5% and 8% of animals were found positive to SIT and ELISA, respectively. Higher prevalence was observed in the age group above 5 years as 5.84% and 9.49% by SIT and ELISA, respectively. Also, the prevalence was found higher in crossbred cattle as 12.06% and 13.79% by SIT and ELISA, respectively. For paratuberculosis in cattle, comparison of results for SIT and ELISA tests was found statistically non-significant (p>0.05) with respect to age. Concerning breed, comparison of results for both tests was found statistically significant (p<0.05) in cattle. The kappa value to know the agreement between SIT and ELISA test was 0.48 for paratuberculosis in cattle. It shows an intermediate to good agreement between both the tests. Overall ELISA was found more sensitive than SIT test for diagnosis of paratuberculosis. Study shows the prevalence of paratuberculosis in cattle of Gaushala in Jaipur, which was found more in the crossbred and higher age group of animals


Aly S, Wagner K, Keller C, Malm S and Malzan A (2006). Oxygen status of lung granulomas in Mycobacterium tuberculosis-infected mice. Journal of Pathology 210:298-305.

Bhati Anita, Shringi Archana, Vyas Indu, Mandovera Vijay and Rani Sunita (2013). A study of pathological conditions in buffalo intestine. Ruminant Science 2(1):43-44.

Boelaert F, Walravens K, Biront P, Vermeersch JP, Berkvens D and Godfroid J (2000). Prevalence of paratuberculosis (Johne’s disease) in the Belgian cattle population. Veterinary Microbiology 77:269-281.

Celik A and Turutoglu H (2017). Seroprevalence of paratuberculosis in cattle, sheep and goats in Burdur, Southwestern Turkey. Israel Journal of Veterinary Medicine 72:1.

Chi J, VanLeeuwen JA, Weersink A and Keefe A (2002). Direct production losses and treatment costs from bovine viral diarrhoea virus, bovine leukosis virus, Mycobacterium avium subspecies paratuberculosis and Neosporacaninum. Preventive Veterinary Medicine 55:137-153.

Didugu H, Ramanipushpa RN, Reddy CEN, Devi MA and Bhaskraramarajusagi S (2015). Mycobacterium avium subspecies paratuberculosis among bovines in Krishna district of Andhra Pradesh, India: A serological survey. Indian Journal of Animal Health 54:149-54.

Dieguez FJ, Arnaiz I, Sanjuan ML, Vilar MJ and Yus E (2008). Management practices associated with Mycobacterium avium subspecies paratuberculosis infection and the effects of the infection on dairy herds. Veterinary Record 162:614-17.

Dutta P, Harikumar AV, Shroff S, Bhattacharya K and Sharma GK (2015). A study on prevalence of bovine paratuberculosis in semen station in India. International Journal of Advanced Veterinary Science and Technology 4(1):195-198.

Filia G, Mahajan V, Devi LG, Bal MS and Singh A (2016). Seroprevalence of paratuberculosis in rural bovine herds from different agro-climatic zones of Punjab. Journal of Animal Research 6(4):751-754.

Ganesan PI (2013). Prevalence of tuberculosis, Johne’sdisease and brucellosis in elite Jersey cows in selected districts of Tamil Nadu. Indian Veterinary Journal 90(8):107-108.

Geisbauer E, Khol JL, Wassertheurer M, Damoser J, Österreicher E, Dünser M, Revilla Fernández S and Baumgartne W (2007). Longterm investigation in an Austrian dairy herd with low prevalence of paratuberculosis detection of antibodies in blood and milk. Veterinary Quarterly 29(4):138-148.

Hendrick S, Duffield T, Leslie K, Lissemore K, Archambault M and Kelton D (2005).The prevalence of milk and serum antibodies to Mycobacterium avium subspecies paratuberculosis in dairy herds in Ontario. Canadian Veterinary Journal 46:1126-1129.

Katoch S, Dohru S, Sharma M, Vashist V, Chahota R, Dhar P, Thakur A and Verma S (2017). Seroprevalence of viral and bacterial diseases among the bovines in Himachal Pradesh, India. Veterinary World 10(12):1421-1426.

Mohan A, Das P, Kushwaha N, Karthik K and Niranjan AK (2013). Investigation on the status of Johne’s disease based on agar gel immunodiffusion, Ziehl-Neelsen staining and nested PCR approach in two cattle farms. Veterinary World 6(10):778-784.

Mohan MS, Duraisamy P, Ezhil PP, Sivakumar P, Tripathi BN and Singh N (2009). Prevalence of paratuberculosis in cattle and buffaloes. Indian Veterinary Journal 86:4-6.

Narnaware SD and Tripathi BN (2017). Seroepidemiology of paratuberculosis in cattle population of organized and unorganized farms of India. Indian Journal of Animal Sciences 87(1):21-24.

NcNab WB, Meek AH, Duncan JR, Martin SW and Dreumel AAV (1991). An epidemiological study of paratuberculosis in dairy cattle in Ontario: Study design and prevalence estimates. Canadian Journal of Veterinary Research 55:246-251

OIE (2021). Terrestrial Manual. Chapter 3.1.15 Paratuberculosis (Johne’s disease).

Thrusfield M (2005). Veterinary Epidemiology. Blackwell Science Ltd., Oxford, UK.

Ott SL, Wells SJ and Wagner BA (1999). Herd-level economic losses associated with Johne’s disease on US dairy operations. Preventive Veterinary Medicine 40:179-192.

Rehman A, Javed MT, Rizvi F and Khan MN (2017). Prevalence of paratuberculosis in cattle and buffaloes in Faisalabad and associated risk factors. Journal of Animal and Plant Science 27(6).

Roupie V, Viart S, Leroy B, Romano M and Trinchero N (2012). Immunogenicity of eight Mycobacterium avium subsp. paratuberculosis specific antigens in DNA vaccinated and Map infected mice. Veterinary Immunology and Immunopathology 15:74-85.

Sahzad, Gupta Saurabh, Chaubey Kundan Kumar, Jayaraman Sujatha, Singh Manju, Stephan Bjorn John, Dayal Deen, Jain Mukta, Pachoori Anjali, Sohal Jagdip Singh, Bhatia Ashok Kumar and Singh Shoor Vir (2015). Comparative evaluation of two screening tests (serum elisa and fecal microscopy) for the estimation of Johne’s disease infection in goat herds endemically infected with Johne’s disease. Ruminant Science 4(2):171-174.

Sharma G, Singh SV, Sevilla I, Singh AV, Whittington RJ, Juste RA, Kumar S, Gupta VK, Singh P, Sohal JS and Vihan VS (2008). Evaluation of indigenous milk ELISA with m-culture and m-PCR for the diagnosis of bovine Johne’s disease (BJD) in lactating Indian dairy cattle. Research in Veterinary Science 84:30-37.

Shivakumar P, Singh N, Tripathi BN, Praveena PE and Saravanan D (2005). Seroprevalence of paratuberculosis infection in cattle. International Journal of Cow Science 1(2):65-67.

Singh SV, Singh AV, Singh R, Sharma S, Shukla N, Misra S, Singh PK, Sohal JS, Kumar H, Patil PK, Misra P and Sandhu KS (2008). Seroprevalence of Johne’s disease in buffaloes and cattle population of North India using indigenous ELISA kit based on native Mycobacterium avium subspecies paratuberculosis ‘Bison type’ genotype of goat origin. Comparative Immunology, Microbiology and Infectious Diseases 31:419-433.

Smith RL, Grohn YT, Pradhan AK, Whitlock RH and Van Kessel JS (2009). A longitudinal study on the impact of Johne’s disease status on milk production in individual cows. Journal of Dairy Science 92:2653-2661.

Stott AW, Jones GM, Humphry RW and Gunn GJ (2005). Financial incentive to control paratuberculosis (Johne’s disease) on dairy farms in the United Kingdom. Veterinary Record 156:825-831.

Thrusfield M (2005). Veterinary Epidemiology. Blackwell Science Ltd, Oxford, UK.

Trangadia BJ, Rana SK, Nagmani K and Srinivasan VA (2012). Serological investigation of ovine brucellosis, Johne’s disease and infectious bovine rhinotracheitis in two states of India. Journal of Advanced Veterinary Research 2(1):38-41.

Tripathi BN, Sonawane GG, Munjal SK, Bind RB, Gradinaru D, Dubey SC, Tripathi S, Mondal D, Paliwal OP and Singh N (2007). Seroprevalence of paratuberculosis in selected population of ruminants in India. In: Proceedings of 9ICP – 2007. pp 246-249.

Vinodhkumar OR and Ganesan PI (2011). Status of bovine paratuberculosis in organized farms in Tamil Nadu. Indian Veterinary Journal 88(11):90-91.

Wells SJ and Wagner BA (2000). Herd-level risk factors for infection with Mycobacterium paratuberculosis in US dairies and association between familiarity of the herd manager with the disease or prior diagnosis of the disease in that herd and use of preventive measures. Journal of American Veterinary Medical Association 216:1450-1457.