28-Title: Effect of aloe vera (Aloe barbadensis) supplementation as feed additive on haematological parameters in Murrah buffalo calves

28-Title: Effect of aloe vera (Aloe barbadensis) supplementation as feed additive on haematological parameters in Murrah buffalo calves

Authors: R Saini, SK Chhikara, S Sahu, S Sihag, DC Yadav and Amandeep

Source: Ruminant Science (2021)-10(2):389-394.

How to cite this manuscript: Saini R, Chhikara SK, Sahu S, Sihag S, Yadav DC and Amandeep (2021). Effect of aloe vera (Aloe barbadensis) supplementation as feed additive on haematological parameters in Murrah buffalo calves. Ruminant Science 10(2):389-394.

Abstract

The present experiment aimed to study the effect of dietary supplementation of fresh aloe vera (Aloe barbadensis) leaves, on haematological parameters in Murrah buffalo calves in tropical India. The study was conducted on 15 buffalo calves (3-6 months age) that were divided into 3 groups of 5 calves each based on nearness in age and body weight. The calves were fed different dietary treatments viz. T1 as Control (basal diet), T2 (basal diet with aloe vera leaves @ 2 g/kg BW) and T3 (basal diet with aloe vera leaves @ 4 g/kg BW) according to ICAR (2013) feeding standards for the duration of 90 days. At the monthly interval, blood samples were collected and analyzed for different blood indices (Hb, TEC, TLC, DLC, PCV, MCV, MCH and MCHC). The results of the study indicated that the mean values of TEC, Hb, PCV, TLC, monocyte and lymphocyte were found statistically higher (P<0.05) in T3 group as compared to T1 group. The mean eosinophil and neutrophil percentages were observed significantly lower (P<0.05) in T3 group as compared to T1 group at the end of the study. The mean value of basophil, MCV and MCHC did not differ significantly (P>0.05) during the experiment among the various treatment groups. The increased values of Hb, TLC, TEC, PCV, lymphocyte and monocyte have potentiated the immune system of calves in the higher aloe vera supplemented group and thus resulted in improved overall health.

References

Anonymous. Govt of India (2012). 19th Livestock census all India report. Ministry of Agriculture Department of Animal Husbandary, Dairying and Fisheries, Krishi Bhavan, New Delhi.

AOAC (2013). Official Methods of Analysis. 19th Edn, Association of Official Analytical Chemists. Arlington Virginia, USA.

Barman S, Samanta AK and Boro PR (2019). Effect of dietary supplementation of aloe vera and Lactobacillus acidophilus on haematological, biochemical and immune parameters of broiler birds. International Journal of Chemical Studies 7(1):1604-1611.

Bhati M (2001). Effect of feeding Bacopa monnieri and aloe vera herbs as feed additives on nutrient utilization efficiency in Rathi calves. MVSc thesis submitted to RAJUVAS, Bikaner.

Cartes KF, Coronado M, Hernandej AJ, Allende ML and Feijoo CJ (2019). Anti-inflammatory effects of aloe vera on soy meal-induced intestinal inflammation in zebrafish. Fish and Shellfish Immunology 95:564-573.

Channa AA, Qazi IH, Soomro SA, Shah AH, Gandahi JA, Korejo RA, Shah IA, Kalhoro NK and Khaskeli BEA (2014). Effect of oral supplements of aloe vera extract on hematology and immune cells in rabbits.  African Journal of Pharmacy and Pharmacology 8(19):497-501.

Choi S and Chung (2003). A review on the relationship between Aloe vera components and their biologic effects. Seminars in Internal Medicine 1:53-62.

Duncan DB (1955). Multiple range and multiple F tests. Biometrics 11(1):1-42.

Economic Survey (2019-2020). Agriculture and food management. Department of Economic Affairs, Government of India, New Delhi. Accessed 10 Apr. 2021. https://www.indiabudget.gov.in/ economicsurvey/doc/vol2chapter/echap07_vol2.pdf

Giannakoudakis DA, Hosseini-Bandegharaei A, Tsafrakidou P, Triantafyllidis KS, Kornaros M and Anastopoulos I (2018).  Aloe vera waste biomass-based adsorbents for the removal of aquatic pollutants: A review. Journal of Environmental Management 227:354–364.

Hamman JH (2008). Composition and applications of aloe vera leaf gel. Molecules 13(8):1599-1616.

ICAR (2013). Nutrient Requirements of Animals- Cattle and Buffalo. 3rd Edn, Indian Council of Agricultural Research, Pusha, New Delhi.

Im SA, Oh ST, Song S, Kim MR, Kim DS and Woo SS (2005). Identification of optimal molecular size of modified Aloe polysaccharides with maximum immunomodulatory activity. International Immunopharmacology 5:271-279.

Kumar R, Kumar S, Palod J, Tewari H and Singh B (2017). Effect of chedu and mattha on growth performance of Murrah buffalo calves. Indian Journal of Animal Research 51(5):906-909.

Kumar S, Kumar S, Dar AH, Palod J, Singh SK and Kumar A (2018). Role of Aloe barbadensis supplementation on haematological parameters and rumen development of crossbred calves. Journal of Entomology and Zoology Studies 6(5):2261-2264.

Leung MYK, Liu C, Zhu LF, Hui YZ, Yu B and Fung KP (2004). Chemical and biological characterization of a polysaccharide biological response modifier from Aloe vera L. var. chinensis (Haw). Berg. Glycobiology 14:501-510.

Maan AA, Nazir A, Khan MKI, Ahmad T, Zia R, Murid  M and Abrar M (2018). The therapeutic properties and applications of Aloe vera: A review. Journal of Herbal Medicine 12:1-10.

Mahdavi A, Alemi F, Ghazvinian K, Ghaderi M and Darabighane B (2012). Study of effects of different levels of Aloe vera gel powder on antibody titre against sheep red blood cells and other blood parameters in broilers. British Poultry Abstracts 8:49-50.

Mushtaq M, Durrani FR, Imtiaz N, Sadique U, Hafeez A, Akhtar S and Ahmad S (2012). Effect of administration of Withania somnifera on some haematological and immunological profile of broiler chicks. Pakistan Veterinary Journal 32(1):70-72.

Nehru PA, Sunandhadevi S, Rama T and Muniyappan N (2017). Effect of probiotic supplementation on growth performance of crossbred calves in an organized cattle farm. Journal of Animal Health and Production 5(3):89-91.

Ni Y, Turner D, Yates KM and Tizard I (2004). Isolation and characterization of structural components of Aloe vera L. leaf pulp. International Immunopharmacology 4:1745-1755.

Oishi K, Susanna H, George A, Diaz Tartania B, Deepa M and Lily Ng (2002). Targeted disruption of Slc19a2, the gene encoding the high-affinity thiamin transporter Thtr-1, causes diabetes mellitus, sensorineural deafness and megaloblastosis in mice. Human Molecular Genetics 11:2951-2960.

Qiao J, Li HH, Zheng ChJ, Feng ZY and Wang W (2013). Dietary supplementation with aloe vera polysaccharide enhances the growth performance and immune function of weaned piglets. Journal of Animal and Feed Sciences 22:329-334.

Saxena N, Mohan Chander, Sharma ML, Dixit VB, Mudgal Vishal and Lal Dalip (2014). Effects of strategic supplementation to male buffalo calves in the peri urban areas of Hisar. Ruminant Science 3(2):195-197.

Sharma P, Kharkwal AC, Kharkwal H, Abdin MZ and Varma A (2014). A review on pharmacological properties of Aloe vera. International Journal of Pharmaceutical Sciences 29(2):31-37.

Snedecor GW and Cochran WG (1994). Statistical Methods. 6th Edn, The Iowa State University Press, Ames, Iowa, USA.

Valle-Paraso M, Vidamo P, Anunciado R and Lapitan A (2005). Effects of aloe vera (Aloe barbadensis) on the white blood cell count and antibody titre of broiler chickens vaccinated against Newcastle disease. Philippine  Journal of Veterinary Medicine 42:49-52.

Verma Abhishek, Kumar Dharmendra, Nema Anuradha, Sheikh TJ, Shukla Mansi, Ranjan Rajesh and Jain Payal (2020). Studies on the efficacy of fresh Aloe vera pulp on wound healing in calves. Ruminant Science 9(1):155-158.

Yadav DC, Bidhan DS, Sihag SS, Sahu S and Sigroha R (2017). Effect of supplementation of different forms of aloe vera (Aloe barbadensis) on the haemato-biochemical and serum mineral profile of broiler Chickens. Indian Journal of Poultry Science 52(1):53-57.