3-Title: Phenotypic and genotypic characterization of extended spectrum beta-lactamases producing Klebsiella pneumoniae in bovines from Jammu region, India

3-Title: Phenotypic and genotypic characterization of extended spectrum beta-lactamases producing Klebsiella pneumoniae in bovines from Jammu region, India

Authors: Deep Shikha, Virendera Singh Wazir, Mohd Rashid and Sabahat Gazal

Source: Ruminant Science (2021)-10(2):253-259.

How to cite this manuscript: Shikha Deep, Wazir Virendera Singh, Rashid Mohd and Gazal Sabahat (2021). Phenotypic and genotypic characterization of extended spectrum beta-lactamases producing Klebsiella pneumoniae in bovines from Jammu region, India. Ruminant Science 10(2):253-259.


The study was conducted to determine the phenotypic and genotypic characterization of Extended Spectrum Beta-Lactamases producing Klebsiella in bovines from Jammu region, India. Out of the total 30 ESBL isolates, resistance to cefotaxime and ceftazidime was observed in 60.0% (15) and 40.0% (10) isolates, respectively while 64.0% (16) isolates showed resistance to both. The present study revealed that the total prevalence of ESBL producing Klebsiella pneumonia in bovines of the Jammu region is 83.33% and is reported for the first time in Jammu region. Out of ESBL isolates, only 44.0% (11) isolates carried the gene/s for blaTEM, blaCTX-M and blaSHV genes by m-PCR. Out of 11 isolates, only 18.18% (2) isolates carried blaTEM gene alone, 54.54% (6) isolates carried blaCTX-M gene alone, 18.18% (2) isolates carried both blaTEM/blaCTX-M genes and only 9.09% (1) isolates carried blaSHV /blaTEM /blaCTX-M. Of the six blaCTX-M positive isolates, 50.0% (3) belonged to CTX-M-1 group, 33.33% (2) to CTX-M-9 group and 16.66% (1) isolates carried both blaCTX-M 1 / blaCTX-M 9. One representative amplicon of blaSHV, blaTEM and blaCTX-M were cloned in pGEM-T and pJET1.2 and got sequenced. The sequence of blaCTX-M was found to match with the database sequence of blaCTX-M -15 and is the first report of its kind in India. Antimicrobial sensitivity test of 11 ESBL producing Klebsiella isolates showed that resistance was most commonly to ampicillin (100%), cefexime (100%), neomycin (100%) followed by streptomycin (90.90%). Antibiotic chloramphenicol (81.81%) and imipenem (72.72%) show sensitive results. Out of 11 multi-drug resistance ESBL positive isolates, only 07 isolates tested positive for class 1 and 2 integrons. Among the 07, 57.14% (4) isolates harbour intI 1 gene, while 28.57% (2) isolates carried intI 2 gene. One (14.28%) isolate, was found to harbour both intI 1 and intI 2.


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