35-Title: Anthelmintic activity of Saraca asoca (roxb.) bark extracts against benzimidazole resistant gastrointestinal nematodes of goats

35-Title: Anthelmintic activity of Saraca asoca (roxb.) bark extracts against benzimidazole resistant gastrointestinal nematodes of goats

Authors: Siddhant Bendigeri, Giridhari Das, Suman Kumar, Rupesh Verma and Rupanjali Saiyam

Source: Ruminant Science (2021)-10(1):177-182.

How to cite this manuscript: Bendigeri Siddhant, Das Giridhari, Kumar Suman, Verma Rupesh and Saiyam Rupanjali (2021). Anthelmintic activity of Saraca asoca (roxb.) bark extracts against benzimidazole resistant gastrointestinal nematodes of goats. Ruminant Science 10(1):177-182.


Anthelmintic activity of aqueous and methanolic extracts of Saraca asoca (Roxb.) bark were evaluated against benzimidazole resistant gastrointestinal nematodes of goats by using both in vitro tests viz. egg hatch test (EHT), larval paralysis test (LPT) and adult mortality test (AMT) and in vivo test, faecal egg count reduction test (FECRT). EHT revealed 100 per cent efficacy at 100 mg/ml (10%), 50 mg/ml (5%) and 25 mg/ml (2.5%) concentration of methanolic and aqueous extracts of the bark. Aqueous extract showed better ED50 (0.156 mg/ml) as compared to methanolic extract (0.357 mg/ml). LPT revealed better efficacy for methanolic extract (99.39% at 100 mg/ml concentration) as compared to aqueous extract (61.06% at 100 mg/ml concentration). Methanolic extract showed better ED50 value (0.552 mg/ml) as compared to aqueous extract (3.050 mg/ml). In AMT, 100 per cent mortality of adult worms was observed at concentrations of 100 mg/ml (10%), 50 mg/ml (5%), 25 mg/ml (2.5%), 12.5 mg/ml (1.25%) and 6.25 mg/ml (0.625%) for methanolic extract and at 100 mg/ml (10%) for aqueous extract.  In in-vivo test, adult goats having egg per gram of faeces (EPG)>300 were selected for the study. A significant reduction (p<0.01)) was observed on day 14 (75.00%) post-treatment in goats treated with methanolic extract of bark as compared to the untreated control. No significant reduction was observed in goats treated with aqueous extract as compared to the control. In the present study, the methanolic extract of Saraca asoca showed better efficacy as compared to aqueous extract.


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