37-Title: Ameliorative effect of Withania somnifera on oxidative stress of crossbred cows suffered with subclinical mastitis

37-Title: Ameliorative effect of Withania somnifera on oxidative stress of crossbred cows suffered with subclinical mastitis

Authors: JP Kachhawa, AP Singh, SR Gupta, ML Sain, Rajendra Yadav, TC Nayak and Sunita Choudhary

Source: Ruminant Science (2022)-11(2):441-444.

How to cite this manuscript: Kachhawa JP, Singh AP, Gupta SR, Sain ML, Yadav Rajendra, Nayak TC and Choudhary Sunita (2022). Ameliorative effect of Withania somnifera on oxidative stress of crossbred cows suffered with subclinical mastitis. Ruminant Science 11(2):441-444.


The present study aimed to determine the effect of Withania somnifera (WS) on oxidative stress in subclinical mastitis in crossbred cows. For this milk samples from apparently healthy Holstein Friesian crossbred cows were collected and examined for subclinical mastitis (SCM) by the CMT, EC, pH, SCC and cultural examination. The effect of WS on oxidative stress-related biochemical parameters including malondialdehyde, reduced glutathione and catalase were evaluated. The eight cows affected with SCM were administered oral alcoholic extract of WS @ 125 mg/kg body weight and intramammary 500 mg WS extract dissolved in 5 ml NSS twice daily for 7 days. The results of this study showed that mean values of CMT point score, SCC, pH, EC and oxidative stress-related biomolecules were improved significantly after the treatment in comparison to pre-treatment values which indicated that the WS alcoholic extract possesses antioxidative property.  It was concluded that the herbal extract of WS possesses antioxidative and therapeutic impact to treat SCM.


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