37-Title: Pharmacokinetics of cefquinome after a single intramuscular injection in cows with clinical dystocia

37-Title: Pharmacokinetics of cefquinome after a single intramuscular injection in cows with clinical dystocia

Authors: Kishor Kumar DG, Rajdeep Kaur, Navdeep Singh, Suresh Kumar Sharma                                     and Simrat Pal Singh Saini

Source: Ruminant Science (2022)-11(1):181-185.

How to cite this manuscript: Kumar DG Kishor, Kaur Rajdeep, Singh Navdeep, Sharma Suresh Kumar and Saini Simrat Pal Singh (2022). Pharmacokinetics of cefquinome after a single intramuscular injection in cows with clinical dystocia. Ruminant Science 11(1):181-185.


The pharmacokinetics of cefquinome in cows (n=6) with clinical dystocia was studied following single intramuscular (IM) administration (1 mg.kg-1 bodyweight). Blood samples were collected prior to and up to 24 h after drug administration. No adverse effects or changes were observed after the IM injection of cefquinome. Plasma concentrations of cefquinome were determined by high-performance liquid chromatography. The plasma concentration-time curves following IM administration were best described by one-compartment open model. The important pharmacokinetic parameters after IM administration analyzed (Mean±SE) were maximum plasma concentration (Cmax) 3.54±0.10 µg.ml-1,  time to achieve Cmax (tmax) 1 h, absorption rate constant (Ka) 5.53±0.36 h-1, absorption half-life (t1/2ka) 0.12±0.008 h, elimination rate constant (ß) 0.61±0.05 h-1, elimination half-life (t1/2ß)1.12±0.009 h, apparent volume of distribution (Vdarea) 0.13±0.004 L.kg-1, area under the concentrationtime curve (AUC) 12.33±0.41 µg.ml-1.h and mean residential time (MRT) 2.39±0.004 h. The in vitro plasma protein binding of cefquinome was 12.56±0.56% at plasma concentrations between 1.25-20.00 µg.ml-1. Cefquinome at a dose of 1 mg.kg-1 body weight by IM administration was sufficient to maintain % T>MIC above 50 % for bacteria having MIC900.50 µg.ml-1 only for a period of 8h in cattle with clinical dystocia.




Al-Taher A (2010). Pharmacokinetics of cefquinome in camels. Journal of Animal and Veterinary Advances 9:848-52.

Amiridis GS, Fthenakis GC, Dafopoulos J, Papanicolaou T and Mavrogianni VS (2003). Use of cefquinome for prevention and treatment of bovine endometritis. Journal of Veterinary Pharmacology and Therapeutics 26:387-90.

Champawat M, Sankhala LN, Sharma P and Gaur A (2018). Effect of flunixin co-administration on pharmacokinetics of cefquinome following intramuscular administration in goats. The Pharma Innovation 7(5 Part G):480.

Craig WA (1995). Interrelationship between pharmacokinetics and pharmacodynamics in determining dosage regimens for broad-spectrum cephalosporins. Diagnostic Microbiology and Infectious Disease 22(1-2):89-96.

Dinakaran V, Dumka VK, Ranjan B, Balaje R and Sidhu PK (2013). Pharmacokinetics following intravenous administration and pharmacodynamics of cefquinome in buffalo calves. Tropical Animal Health and Production 45(7):1509-12.

Dumka VK, Dinakaran V, Ranjan B and Rampal S (2013). Comparative pharmacokinetics of cefquinome following intravenous and intramuscular administration in goats. Small Ruminant Research 113(1):273-77.

Gibaldi M and Perrier D (1982). Pharmacokinetics. 2nd Edn, Marcel and Dekker Inc. New York.

Gupta A, Hammarlund-Udenaes M, Chatelain P, Massingham R and Jonsson EN (2006). Stereoselective pharmacokinetics of cetirizine in the guinea pig: Role of protein binding. Biopharmaceutics and Drug Disposition 27(6):291-297.

Holtgrew-Bohling KJ (2014). Large Animal Clinical Procedures for Veterinary Technicians- E-Book. Elsevier Health Sciences.

Li YF, Lin W, Gu XY, Zeng Z, Fan Y and Ding HZ (2014). Pharmacokinetics and residues of cefquinome in milk of lactating Chinese dairy cows after intramammary administration. Journal of Integrative Agriculture 13(12):2750-57.

Limbert M, Isert D, Klesel N, Markus A, Seeger K, Seibert G and Schrinner E (1991). Antibacterial activities in vitro and in vivo and pharmacokinetics of cefquinome (HR 111V), a new broad-spectrum cephalosporin. Antimicrobial Agents and Chemotherapy 35:14-19.

Mangal M (2013). Pharmacokinetic and Toxicological Studies of cefquinome in buffalo calves. Doctoral dissertation submitted to GADVASU, Ludhiana.

Patil MK, Somkuwar AP and Patil PV (2020). Pharmacokinetics of cefquinome in Marathwadi buffalo calves. Ruminant Science 9(2):229-232.

Pohl A, Lübke-Becker A and Heuwieser W (2018). Minimum inhibitory concentrations of frequently used antibiotics against Escherichia coli and Trueperella pyogenes isolated from uteri of postpartum dairy cows. Journal of Dairy Science 101(2):1355-1364.

Rana MP, Sadariya KA and Thaker AM (2015). Effect of tolfenamic acid co-administration on pharmacokinetics of cefquinome following intramuscular administration in sheep. Veterinarski Arhiv 85(3):283-92.

Singh S (2017). Disposition of cefquinome alone and in combination with tazobactam in cattle suffering from pericarditis. MVSc thesis submitted to GADVASU, Ludhiana, India.

Srivastava AK and Bal MS (1994). Principles and Calculation in Pharmacokinetics. Punjab Agricultural University, Ludhiana, India.

Tohamy MA (2011). Age-related intramuscular pharmacokinetics of cefquinome in sheep. Small Ruminant Research 99:72-76.

Turnidge J (1999). Pharmacokinetics and pharmacodynamics of fluoroquinolones. Drugs 58(2):29-36.

Uney K, Altan F and Elmas M (2011). Development and validation of a high-performance liquid chromatography method for determination of cefquinome concentrations in sheep plasma and its application to pharmacokinetic studies. Antimicrobial Agents and Chemotherapy 55(2):854-59.

Williams EJ, Fischer DP, Pfeiffer DU, England GC, Noakes DE, Dobson H and Sheldon IM (2005). Clinical evaluation of postpartum vaginal mucus reflects uterine bacterial infection and the immune response in cattle. Theriogenology 63(1):102-17.