38-Title: Antibiotic resistance studies in Staphylococcus aureus isolates from goat milk

Authors: QA Juya, P Kaur, M Parmar, NS Sharma and AK Arora

Source: Ruminant Science (2022)-11(2):445-454.

How to cite this manuscript: Juya QA, Kaur P, Parmar M, Sharma NS and Arora AK (2022). Antibiotic resistance studies in Staphylococcus aureus isolates from goat milk. Ruminant Science 11(2):445-454.

Abstract

A total of 150 samples (milk=125 and farm premise samples=25) were collected from mastitic (n=100) as well as apparently healthy (n=25) goats. On isolation, a total of 21 Staphylococcus aureus isolates were obtained, which were confirmed by various biochemical and molecular tests. All 21 isolates exhibited positive results for genus and species-specific PCR. For studying the antibiotic resistance pattern, the isolates were tested for sensitivity against 16 different antibiotics using the disc diffusion method. Sensitivity pattern indicated that the majority of the isolates were resistant to cefoxitin, methicillin (76.1%) and penicillin G (61.9%) and the highest resistance was observed against beta-lactam group. Molecular detection for various antibiotic resistance genes viz. aacA-aph D, blaZ, tetK, tetM, tetL, tetA, ermA, ermB, ermC, vanA, vanB and mec A revealed that tetK (38.09%) and tetM (33.33%) were the predominant resistance genes detected among the isolates, followed by ermC (28.57%), blaZ and ermB (14.29%).

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