4-Title: A novel snp identified in intron1 of superoxide dismutase 1 (SOD1) in Vechur cattle of Kerala

Authors: Katam Divya, Elizabeth Kurian, T Naicy, Manoj M, Hiron M Harshan, Priya M and TV Aravindakshan

Source: Ruminant Science (2021)-10(2):261-264.

How to cite this manuscript: Divya Katam, Kurian Elizabeth, Naicy T, Manoj M, Harshan Hiron M, Priya M and Aravindakshan TV (2021). A novel snp identified in intron1 of superoxide dismutase 1 (SOD1) in Vechur cattle of Kerala. Ruminant Science 10(2):261-264.

Abstract

Vechur, a threatened indigenous cattle breed of Kerala is considered to be one of the smallest cattle in the world. The uniqueness of the breed is a reflection of the distinct allelic combination it possesses. Extensive molecular studies are required to characterise this breed. The genetic polymorphisms could regulate the gene transcription and alter the structure, and function of the translated protein. Genomic DNA was isolated from the whole blood of Vechur cattle (n=78) using the phenol-chloroform extraction method. The coding sequence of SOD1 consists of five exons. This present study was conducted to characterise the gene fragment containing exon 2 with the flanking intronic sequences. The fragment was amplified using polymerase chain reaction (PCR) with custom-designed primers. Sequence analysis revealed the presence of one novel single nucleotide polymorphisms (SNP) g.1992G>A. The population was genotyped using a high-resolution melt curve (HRM) analysis.  Gene and genotype frequencies in the population were estimated.

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