41-Title: Detection of anthelmintic resistance in trichostrongyloid nematodes of goats reared under semi-intensive system
Authors: Jitendra Tiwari, Daya Shanker and Aniruddha Singh Niranjan
Source: Ruminant Science (2022)-11(2):465-470.
How to cite this manuscript: Tiwari Jitendra, Shanker Daya and Niranjan Aniruddha Singh (2022). Detection of anthelmintic resistance in trichostrongyloid nematodes of goats reared under semi-intensive system. Ruminant Science 11(2):465-470.
Abstract
The present study was conducted to reveal the status of anthelmintic resistance in Gastro Intestinal Nematodes (GIN) of goats reared under semi-intensive system. Sixteen herds of eight villages of Mathura were considered for their parasitic load and the efficacy of fenbendazole was evaluated using in-vitro Egg Hatch Assay (EHA) and PCR-RFLP test. The qualitative examination revealed the presence of Haemonchus, Trichostrongylus, Oesophagostomum, Trichuris and Strongyloides nematodes in the faecal samples. Log-probit analysis of EHA results of eight villages revealed the effective dose (ED50) in the range of 0.093 to 0.215 µg/ml. Post-treatment larvae population consists only of Haemonchus contortus indicating resistance development only due to this parasite whereas other worm species are still susceptible to benzimidazoles. Results of EHA indicate the emergence of benzimidazole resistance in five villages with an ED50 value of more than the discriminating dose 0.1 ìg/ml. The more sensitive molecular test revealed the presence of benzimidazole resistance in H. contortus in seven out of eight villages under the study. The percentage of homozygous resistant (RR) and heterozygous resistant (RS) populations of H. contortus in different villages showed variation from 5-20 per cent and 10-40 per cent, respectively. The overall frequency of the resistant (R) allele and susceptible allele (S) was 0.225 and 0.775, respectively. No resistance was recorded in Trichostrongylus spp. of nematode. The study evidences the presence of benzimidazole resistance in H. contortus worm of goats in the Mathura district. The results suggest the need for large-scale surveillance in other regions and the implementation of sustainable control strategies against GIN.
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