43-Title: Effect of resveratrol supplementation on the semen quality of cryopreserved Hariana bull semen

Authors: Mahesh Kumar, Anuj Kumar, Vikas Sachan, JK Agarwal, Atul Saxena, Avaneesh Kumar Singh, Shashi Kant Gupta and Dilip Swain

Source: Ruminant Science (2022)-11(2):475-478.

How to cite this manuscript: Kumar Mahesh, Kumar Anuj, Sachan Vikas, Agarwal JK, Saxena Atul, Singh Avaneesh Kumar, Gupta Shashi Kant and Swain Dilip (2022). Effect of resveratrol supplementation on the semen quality of cryopreserved Hariana bull semen. Ruminant Science 11(2):475-478.

Abstract

The present study was designed to determine the effect of resveratrol as an additive in tris-egg yolk-based extender in Hariana bull semen opted for cryopreservation. The study evaluated physico-morphological properties (individual progressive motility, viability, Hypoosmotic swelling (HOS) response, acrosomal integrity, at pre-freeze and post-thaw stages of cryopreservation. Thirty-two ejaculates collected from four Hariana bulls were divided into four aliquots. Aliquot diluted in egg yolk tris glycerol (EYTG), Group I: control (without addition of resveratrol), Group II: treated with 0.5 mM resveratrol, Group III: treated with 1.0 mM resveratrol, Group IV: treated with 2.0 mM resveratrol. Semen evaluated at pre-freeze and post-thaw stages showed that supplementation of 0.5 mM resveratrol in semen significantly (p<0.05) increase individual progressive motility (%), viability (%), acrosomal integrity (%), and HOS response (%) at both stages of cryopreservation.

References

Ahmed H, Jahan S, Ullah H, Ullah F, Salman MM (2020). The addition of resveratrol in tris citric acid extender ameliorates post-thaw quality parameters, antioxidant enzymes levels and fertilizing capacity of buffalo (Bubalus bubalis) bull spermatozoa. Theriogenology 152:106-113.

Assuncao MC, Mendes VRA, Brandao FZ, Batista RITP, Souza ED, Carvalho BCD, Quintao CCR, Raposo NRB, Camargo LSA (2021). Effect of resveratrol in bull semen extender on post-thaw sperm quality and capacity for fertilization and embryo development. Animal Reproduction Science 226:106-697.

Bucak MN, Baþpýnar N, Tuncer PB, Coyan K, Sarýozkan S, Akalýn PP, Buyukleblebici S and Kuçukgunay S (2014). Effects of curcumin and dithioerythritol on frozen-thawed bovine semen. Andrologia 44:102-109.

Chaudhary KF, Suthar BN, Nakhashi HC and Mohapatra SK (2022). Effect of curcumin as a semen additive on seminal attributes and biochemical parameters of Kankrej bull spermatozoa. Ruminant Science 11(1):57-62.

Fulda S, Galluzzi L and Kroemer G (2010). Targeting mitochondria for cancer therapy. Nature Reviews Drug Discovery 9:447-64.

Garcez ME, Branco CD, Lara LV, Pasqualotto FF and Salvador M (2010). Effects of resveratrol supplementation on cryopreservation medium of human semen. Fertility and Sterility 94(6):2118-2021.

Garrido N, Meseguer M, Simon C, Pellicer A and Remohi J (2004). Pro-oxidative and anti-oxidative imbalance in human semen and its relation with male fertility. Asian Journal of Andrology 6(1):59-66.

Holt WV (2000). Fundamental aspects of sperm cryobiology: the importance of species and individual differences. Theriogenology 53:47-58.

Jeyendran RS, Vander Ven HH, Parez-pelaez M, Crabo BG and Zaneweld LJD (1984). Development of an assay to assess the functional integrity of the human membrane and its relationship to other semen characteristics. Journal of Reproduction and Fertility 70:219-228.

Kapadiya PS, Nakhashi HC, Chauhan PM, Sutaria TV and Suthar BN (2018). Efficacy of melatonin as an antioxidant for cryopreservation of Kankrej bull semen. Ruminant Science 7(1):89-92.

Kaur Navjot, Sharma Manjinder, Lonare MK and Singh Digvijay (2020). Cytoprotective potential of curcumin and resveratrol during freeze-thaw cycle of buffalo bone marrow derived mesenchymal stem cells. Ruminant Science 9(2):215-222.

Leonard SS, Xia C, Jiang BH, Stinefelt B, Klandorf H, Harris GK and Shi X (2003). Resveratrol scavenges reactive oxygen species and effects radical-induced cellular responses. Biochemical and Biophysical Research Communications 309:1017-1026.

Lv C, Larbi, Allai Wu, Guoquan Hong, Qionghua Quan and Guobo (2019). Improving the quality of cryopreserved goat semen with a commercial bull extender supplemented with resveratrol. Animal Reproduction Science 208:106-127.

Nouri H, Shojaeian K, Samadian F, Lee S, Kohram H and Lee JI (2018). Using resveratrol and epigallocatechin-3-gallate to improve cryopreservation of stallion spermatozoa with low quality. Journal of Equine Veterinary Science 70:18-25.

Patel BR and Siddiquee GM (2013). Effect of semen additives on leakage of certain enzymes during cryopreservation of Kankrej semen. Ruminant Science 2(1):63-66.

Pervaiz S and Holme AL (2009). Resveratrol: its biological targets and functional activity. Antioxid Redox Signal 11:2851-97.

Revel A, Raanani H, Younglai E, Xu J, Han R and Savouret JF (2001). Resveratrol a natural aryl hydrocarbon receptor antagonist protects sperm from DNA damage and apoptosis caused by benzopyrene. Reproductive Toxicology 15:479-86.

Sahrawat Alok K, Saxena Atul and Mishra Sanjay K (2013). Cryopreservation of Bhadawari buffalo bull spermatozoa in tris and citrate based extenders. Ruminant Science 2(1):67-70.

Sarlos P, Molnar A, Kokai M, Gabor G and Ratky J (2002). Comparative evaluation of the effect of antioxidants in the conservation of ram semen. Acta Veterinaria Hungarica 50:235-45.

Satish, Purohit GN, Sharma RK, Kumar Pradeep, Jerome A, Mehta JS, Kumar Devender,  Bala Renu, Verma Nisha and Singh Sajjan (2022). Effect of buffalo specific semen extenders on hos test positive sperms at different stages of cryopreservation in Murrah buffalo bulls. Ruminant Science 11(1):235-237.

Shukla MK, Misra AK and Gupta HP (2012). Caffeine as semen additive to improve Murrah buffalo (Bubalus bubalis) semen cryopreservation. Ruminant Science 1(2):121-124.

Silva ECB, Cajueiro JFP, Silva SV, Soares PC and Guerra MMP (2012). Effect of antioxidants resveratrol and quercetin on in vitro evaluation of frozen ram sperm. Theriogenology 77(8):1722-1726.

Yadava Chhote Lal, Saxena Atul, Yadav Brijesh, Singh Vipin, Reddy A Vidhya Sagar, Patel Akhil, Kumar Anuj and Yadav Sarvajeet (2018). Effect of sericin supplementation on the semen quality of cryopreserved hariana bull semen. Ruminant Science 7(1):93-96.

Zhu Z, Li R, Fan X, Lv Y, Zheng Y, Hoque SAM, Wu D and Zeng W (2019). Resveratrol improves boar sperm quality via 5’AMP-Activated protein kinase activation during cryopreservation. Oxidative Medicine and Cellular Longevity. pp 1-15.