48-Title: Effect of buffalo specific semen extenders on hos test positive sperms at different stages of cryopreservation in Murrah buffalo bulls
Authors: Satish, GN Purohit, RK Sharma, Pradeep Kumar, A Jerome, JS Mehta, Devender Kumar, Renu Bala, Nisha Verma and Sajjan Singh
Source: Ruminant Science (2022)-11(1):235-237.
How to cite this manuscript: Satish, Purohit GN, Sharma RK, Kumar Pradeep, Jerome A, Mehta JS, Kumar Devender, Bala Renu, Verma Nisha and Singh Sajjan (2022). Effect of buffalo specific semen extenders on hos test positive sperms at different stages of cryopreservation in Murrah buffalo bulls. Ruminant Science 11(1):235-237.
With an objective to evaluate the best buffalo specific extender, semen was collected from Murrah buffalo bulls (n=6) by artificial vagina and subjected to dilution using buffalo specific extenders Tris egg yolk, CIRB (LDL), GVE (TEYC with 1 mg/mL tocopherol) and GSE (TEYC with 0.25% sericin). Semen was cryopreserved and HOS test-positive sperms were evaluated immediately after dilution, at the equilibration stage and in cryopreserved semen thawed after 24 h. A comparison of HOST reactive sperms at three different stages revealed that the overall HOST reactive sperms were non-significantly different for the different extenders although slightly higher for the CIRB extender. At the equilibration stage and frozen-thawed semen the HOST reactive sperms were significantly higher (P<0.001) for the CIRB extender as compared to TEYC, GSE, and the GVE extender. It was concluded that the LDL-based extender (CIRB) is optimum for the dilution and cryopreservation of buffalo semen.
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