6-Title: Expression profiling of ADAM17 gene in ovary, fallopian tube and uterus of Malabari goat
Authors: CA Dharamshaw, Elizabeth Kurian, T Naicy, TV Aravindakshan, RS Abhilash and R Thirupathy Venkatachalapathy
Source: Ruminant Science (2021)-10(2):271-275.
How to cite this manuscript: Dharamshaw CA, Kurian Elizabeth, Naicy T, Aravindakshan TV, Abhilash RS and Venkatachalapathy R Thirupathy (2021). Expression profiling of ADAM17 gene in ovary, fallopian tube and uterus of Malabari goat. Ruminant Science 10(2):271-275.
The ADAM17 gene regulates the signalling of TGF-ß and TNF-alpha that have a wide variety of functions in the reproductive system. The current study intended to analyse the expression of the ADAM17 gene in the ovary, fallopian tube and uterus of six Malabari does. For differential expression, RNA was extracted and complementary DNA synthesised. Relative quantification was performed by qPCR with GAPDH as the internal control gene. Statistical analysis revealed a significant upregulation of ADAM17 in the uterus (p<0.01) compared to the fallopian tube and ovary. This suggests that ADAM17 may influence uterine functions such as implantation, uterine remodelling and pregnancy maintenance.
Ashkenazi H, Cao X, Motola S, Popliker M, Conti M and Tsafriri A (2005). Epidermal growth factor family members: Endogenous mediators of the ovulatory response. Endocrinology 146(1):77-84.
Black RA, Rauch CT, Kozlosky CJ, Peschon JJ, Slack JL, Wolfson MF, Castner BJ, Stocking KL, Reddy P, Srinivasan S, Nelson N and Cerretti DP (1997). A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells. Nature 385(6618):729-733.
Chegini Nasser, Zhao Yong, Williams RS and Flanders KC (1994). Human uterine tissue throughout the menstrual cycle expresses transforming growth factor-beta 1 (TGF beta 1), TGF beta 2, TGF beta 3, and TGF beta type II receptor messenger ribonucleic acid and protein and contains [125I] TGF beta 1-binding sites. Endocrinology 135(1):439-449.
Chu W, Li X, Li C, Wan L, Shi H, Song X, Liu X, Chen X, Zhang C, Shan H and Lu Y (2011). TGFBR3, a potential negative regulator of TGF-ß signalling, protects cardiac fibroblasts from hypoxia induced apoptosis. Journal of Cellular Physiology 226(10): 2586-2594.
Eppig JJ, Wigglesworth K and Pendola FL (2002). The mammalian oocyte orchestrates the rate of ovarian follicular development. Proceedings of the National Academy of Sciences 99(5):2890-2894.
Feldmann M, Brennan FM, Elliott MJ, Williams RO and Maini RN (1995). TNF-alpha Is an Effective Therapeutic Target for Rheumatoid Arthritis. Annals of the New York Academy of Sciences 766(1):272-278.
Feng M, Wang Q, Zhang F and Lu L (2012). Ex vivo induced regulatory T cells regulate inflammatory response of Kupffer cells by TGF-beta and attenuate liver ischemia reperfusion injury. International Immunopharmacology 12(1):189-196.
Gooz M (2010). ADAM-17: the enzyme that does it all. Critical Reviews in Biochemistry and Molecular Biology 45(2):146-169.
Gooz M, Gooz P, Luttrell LM and Raymond JR (2006). 5-HT2A receptor induces ERK phosphorylation and proliferation through ADAM-17 tumor necrosis factor-alpha-converting enzyme (TACE) activation and heparin-bound epidermal growth factor-like growth factor (HB-EGF) shedding in mesangial cells. Journal of Biological Chemistry 281(30):21004-21012.
Harris RC, Chung E and Coffey RJ (2003). EGF receptor ligands. Experimental Cell Research 284:2-13.
Jowicz AP, Brown JK, McDonald SE, Shaw JL, Critchley HO and Horne AW (2013). Characterization of the temporal and spatial expression of a disintegrin and metalloprotease 17 in the human endometrium and fallopian tube. Reproductive Sciences 20(11):1321-1326.
Livak KJ and Schmittgen TD (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2-ÄÄCT method. Methods 25(4):402-408.
Malapeira J, Esselens C, Bech-Serra JJ, Canals F and Arribas J (2011). ADAM17 (TACE) regulates TGF-ß signalling through the cleavage of vasorin. Oncogene 30(16):1912-1922.
Malik S, Day K, Perrault I, Charnock-Jones DS and Smith SK (2006). Reduced levels of VEGF-A and MMP-2 and MMP-9 activity and increased TNF-alpha in menstrual endometrium and effluent in women with menorrhagia. Human Reproduction 21(8):2158-2166.
Matzuk MM, Burns KH, Viveiros MM and Eppig JJ (2002). Intercellular communication in the mammalian ovary: Oocytes carry the conversation. Science 296(5576):2178-2180.
Monsivais D, Matzuk MM and Pangas SA (2017). The TGF-ß family in the reproductive tract. Cold Spring Harbor Perspectives in Biology 9(10):a022251.
Moss ML, Jin SLC, Milla ME, Burkhart W, Carter HL, Chen WJ, Clay WC, Didsbury JR, Hassler D, Hoffman CR and Kost TA (1997). Cloning of a disintegrin metalloproteinase that processes precursor tumour-necrosis factor-alpha. Nature 385(6618):733-736.
Naicy T, Venkatachalapathy RT, Aravindakshan TV, Radhika G, Raghavan KC, Mini M and Shyama K (2016). Nerve Growth Factor gene ovarian expression, polymorphism identification, and association with litter size in goats. Theriogenology 86(9):2172-2178.
Panigone S, Hsieh M, Fu M, Persani L and Conti M (2008) Luteinizing hormone signalling in preovulatory follicles involves early activation of the epidermal growth factor receptor pathway. Molecular Endocrinology 22(4):924-936.
Park JY, Su YQ, Ariga M, Law E, Jin SL and Conti M (2004). EGF-like growth factors as mediators of LH action in the ovulatory follicle. Science 303(5658):682-684.
Rotwein P (1991). Structure, evolution, expression and regulation of insulin-like growth factors I and II. Growth Factors 5(1):3-18.
Rovida E, Paccagnini A, Del Rosso M, Peschon J and Sbarba PD (2001). TNF-alpha-converting enzyme cleaves the macrophage colony-stimulating factor receptor in macrophages undergoing activation. Journal of Immunology 166(3):1583-1589.
Sadek CM and Allen-Hoffmann BL (1994). Suspension-mediated induction of Hepa 1c1c7 Cyp1a-1 expression is dependent on the Ah receptor signal transduction pathway. Journal of Biological Chemistry 269(50):31505-31509.
Sahin U, Weskamp G, Kelly K, Zhou HM, Higashiyama S, Peschon J, Hartmann D, Saftig P and Blobel CP (2004). Distinct roles for ADAM10 and ADAM17 in ectodomain shedding of six EGFR ligands. Journal of Cell Biology 164(5):769-779.
Sayasith K and Sirois J (2015). Molecular characterization of a disintegrin and metalloprotease-17 (ADAM17) in granulosa cells of bovine preovulatory follicles. Molecular and Cellular Endocrinology 411:49-57.
Vacca P, Cantoni C, Vitale M, Prato C, Canegallo F, Fenoglio D, Ragni N, Moretta L and Mingari MC (2010). Crosstalk between decidual NK and CD14+ myelomonocytic cells results in induction of Tregs and immunosuppression. Proceedings of the National Academy of Sciences 107(26):11918-11923.