8-Title: Molecular detection of Theileria spp. in ticks on goats in Kerala, South India

8-Title: Molecular detection of Theileria spp. in ticks on goats in Kerala, South India

Authors: Hitaishi VN, Bindu Lakshmanan, Jain Jose K, Seph Ross Emmanuel and H Shameem

Source: Ruminant Science (2021)-10(2):281-284.

How to cite this manuscript: Hitaishi VN, Lakshmanan Bindu, Jain Jose K, Emmanuel Seph Ross and Shameem H (2021). Molecular detection of Theileria spp. in ticks on goats in Kerala, South India. Ruminant Science 10(2):281-284.


Ticks are important vectors of parasitic and rickettsial infections of animals and inflict serious economic loss in the livestock sector. Ixodid ticks of the genera Rhipicephalus and Haemaphysalis spp. commonly infest the livestock of Kerala. Though the prevalence of haemoparasitic infection and the extent of tick infestation are alarming among goats in the state, attempts have not been made to provide molecular evidence of these organisms in vectors. Out of the 100 ticks analysed as 21 tick pools, 38.1 per cent were found to harbour Theileria spp. by PCR targeting the 18S rRNA gene. The ticks belonging to Haemaphysalis bispinosa, H. spinigera and Rhipicephalus sanguineus were tested positive for Theileria ovis by PCR-RFLP using Vsp1digestion. Theileria luwenshuni could be identified in one tick belonging to Haemaphysalis spp by species-specific PCR. The present work provided molecular evidence of the most prevalent haemoparasite in tick vectors for the first time in Kerala. Though the presence of the organism in ticks need not establish its vectorial capacity, the study establishes the need to explore the vector status of different tick species for the prevalent haemoparasites in different regions.


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