Title: Effect of environment on gene expression profiling of TNFα in barbari goats (Capra hircus)

Authors: Vijay Pratap Yadav and Anant Rai

Source: Ruminant Science (2016)-5(2):159-164.

Cite this reference as: Yadav Vijay Pratap and Rai Anant (2016). Effect of environment on gene expression profiling of TNFα in barbari goats (Capra hircus). Ruminant Science 5(2):159-164.

Abstract

Present study was design to establish the expression pattern of TNF-α in peripheral blood mononuclear cells (PBMCs) during different climatic temperature variation in goats (Capra hircus). Real-time PCR, SDS page and western blot were applied to investigate mRNA expressions, protein expressions of examined factors. The mRNA and protein expression of TNF-α was significantly higher (P<0.05) during mid of the summer (June) and expression was observed significantly higher (P<0.05) during mid of the winter season (February) as compared to thermo neutral or moderate season (March). Outcome of the proposed study was demonstrated that TNF-α  genes is  expressed in caprine PBMCs and higher expression of these genes during thermal stress suggest possible involvement of this gene to maintain the hemostat of animal during acute thermal stress and actively participate in to ameliorate deleterious effect of thermal stress and maintenance of cellular integrity . It is also interesting to see that this gene also show higher expression in winter season as compare to moderate season, the exact reason of this phenomena was unknown but we hypothesized that TNF-α gene is very sensitive to climate change either hot and cold and it may play an important role in the development and maintenance of the homeostatic in animal. However, the exact precise mechanism of action of this gene in this scenario was unknown and thrust to be investigated how this gene was recognized and select the path to either prevent or apoptosis the cells under stress condition. This study may help investigator for future study in the area of stress physiology in animals.   

References

Bouchama A, Roberts G, Al Mohanna F, El-Sayed R, Lach B, Chollet-Martin S, Ollivier V, Al Baradei R, Loualich A, Nakeeb S, Eldali A and De Prost D  (2005).Inflammatory, hemostatic, and clinical changes in a baboon experimental model for heatstroke. Journal of Applied Physiology 98:697-705.

Bernabucci U, Ronchi B, Lacetera N, Nardone A (2002). Markers of oxidative status in plasma and erythrocytes of transition dairy cows during hot season. Journal of Dairy Science 85:2173-2179.

Baumann H and Gauldie J (1994). The acute phase response. Immunology Today 15(2):74-80.

Cross AS, Sadoff JC, Kelly N, Bernton E and Gemski P (1989). Pretreatment with recombinant murine tumor necrosis factor alpha/cachectin and murine interleukin 1 alpha protects mice from lethal bacterial infection. Journal of Experimental Medicine 169(6):2021-2027.

Cohen MC and Cohen S (1996). Cytokine function: a study in biologic diversity. American Journal of Clinical Pathology 105(5):589-598.

Cross A, Asher L, Seguin M, Yuan LI, Kelly N, Hammack C, Sadoff J and Gemski Jr P (1995). The importance of a lipopolysaccharide-initiated, cytokine-mediated host defense mechanism in mice against extra intestinally invasive Escherichia coli. Journal of Clinical Investigation 96(2):676.

Dangi SS, Gupta M, Nagar V, Yadav VP, Dangi SK, Shankar O, Chouhan VS, Kumar P, Singh G and Sarkar M (2014). Impact of short-term heat stress on physiological responses and expression profile of HSPs in Barbari goats. International Journal of Biometeorology 58(10):2085-2093.

Dugué B and Leppanen E (2000). Adaptation related to cytokines in man-effects of regular swimming in ice-cold water. Clinical Physiology 20:114-121.

Dangi SS, Gupta M, Maurya D, Yadav VP, Panda RP, Singh G, Mohan NH, Bhure SK, Das BC, Bag S and Mahapatra R (2012). Expression profile of HSP genes during different seasons in goats (Capra hircus). Tropical Animal Health and Production 44(8):1905-1912.

Heads RJ, Yellon DM and Latchman DS (1995). Differential cytoprotection against heat stress or hypoxia following expression of specific stress protein genes in myogenic cells. Journal of Molecular and Cellular Cardiology 27(8):1669-1678.

Jiang Q, DeTolla L, van Rooijen N, Singh IS, Fitzgerald B, Lipsky MM, Kane AS, Cross AS and Hasday JD (1999). Febrile-range temperature modifies early systemic tumor necrosis factor alpha expression in mice challenged with bacterial endotoxin. Infection and Immunity 67:1539-1546.

Kluger MJ (1991). Fever: Role of pyrogens and cryogens. Physiological Reviews 7193-127.

Leon LR, Blaha MD and DuBose DA (2006). Time course of cytokine, corticosterone, and tissue injury responses in mice during heat strain recovery. Journal of Applied Physiology 100(4):1400-1409.

Marber MS and Yellon DM (1996) adaptation, stress proteins, and the second window of protection. Annals of the New York Academy of Sciences 793:123-141.

Marai IF and Haeeb AA (2010). Buffalo’s biological functions as affected by heat stress-A review. Livestock Science 127(2):89-109.

Maurya D, Gupta M, Dangi SS, Yadav VP, Mahapatra RK, Sarkar M (2013). Expression of genes associated with thermal stress in goats during different seasons. Indian Journal of Animal Sciences 83(6).

Misra RP and Puneet K (2009). Improved shelter management, feeding and watering devices for goats. Goat enterprises, CIRG, Mathura (UP), India, 132.

Nwaka S, Mechler B, von Ahsen O and Holzer H (1996). The heat shock factor and mitochondrial Hsp70 are necessary for survival of heat shock in Saccharomyces cerevisiae. FEBS Letters 399(3):259-263.

Ostberg JR, Taylor SL, Baumann H and Repasky EA (2000). Regulatory effects of fever-range whole-body hyperthermia on the LPS-induced acute inflammatory response. Journal of Leukocyte Biology 68(6):815-820.

Plumier JC, Robertson HA, Currie WR (1996). Differential accumulation of mRNA for immediate early genes and heat shock genes in heart after ischaemic injury. Journal of Molecular and Cellular Cardiology 28(6):1251-1260.

Pedersen BK and Febbraio MA (2008). Muscle as an

endocrine organ: focus on muscle-derived interleukin-6. Physiological Reviews 88(4):1379-1406.

Pritchard MT, Li Z and Repasky EA (2005). Nitric oxide production is regulated by fever-range thermal stimulation of murine macrophages. Journal of Leukocyte Biology 78(3):630-638.

Panjwani NN, Popova L and Srivastava PK (2002). Heat shock proteins gp96 and hsp70 activate the release of nitric oxide by APCs. Journal of Immunology 168(6):2997-3003.

Suzuki K and Watanabe M (1994). Modulation of cell growth and mutation induction by introduction of the expression vector of human hsp70 gene. Experimental Cell Research 215(1):75-81.

Steiner E, Kleinhappl B, Gutschi A and Marth E (1998). Analysis of hsp70 mRNA levels in HepG2 cells exposed to various metals differing in toxicity. Toxicology Letters 96:169-176.

Vega VL, Rodríguez-Silva M, Frey T, Gehrmann M, Diaz JC, Steinem C, Multhoff G, Arispe N and De Maio A (2008). Hsp70 translocates into the plasma membrane after stress and is released into the extracellular environment in a membrane-associated form that activates macrophages. Journal of Immunology 180(6):4299-4307.

Wu C (1995). Heat shock transcription factors: Structure and regulation. Annual Review of Cell and Developmental Biology 11(1):441-469.

Welc SS, Phillips NA, Oca-Cossio J, Wallet SM, Chen DL and Clanton TL (2012). Hyperthermia increases interleukin-6 in mouse skeletal muscle. American Journal of Physiology Cell Physiology 303(4):C455-C466.

Yadav VP, Dangi SS, Chouhan VS, Gupta M, Dangi SK, Singh G, Maurya VP, Kumar P and Sarkar M (2016). Expression analysis of NOS family and HSP genes during thermal stress in goat (Capra hircus). International Journal of Biometeorology 60(3):381-389.