Effect of environment on gene expression profiling of TNFα in barbari goats (Capra hircus)

Title: Effect of environment on gene expression profiling of TNFα in barbari goats (Capra hircus)

Authors: Vijay Pratap Yadav and Anant Rai

Source: Ruminant Science (2016)-5(2):159-164.

Cite this reference as: Yadav Vijay Pratap and Rai Anant (2016). Effect of environment on gene expression profiling of TNFα in barbari goats (Capra hircus). Ruminant Science 5(2):159-164.


Present study was design to establish the expression pattern of TNF-α in peripheral blood mononuclear cells (PBMCs) during different climatic temperature variation in goats (Capra hircus). Real-time PCR, SDS page and western blot were applied to investigate mRNA expressions, protein expressions of examined factors. The mRNA and protein expression of TNF-α was significantly higher (P<0.05) during mid of the summer (June) and expression was observed significantly higher (P<0.05) during mid of the winter season (February) as compared to thermo neutral or moderate season (March). Outcome of the proposed study was demonstrated that TNF-α  genes is  expressed in caprine PBMCs and higher expression of these genes during thermal stress suggest possible involvement of this gene to maintain the hemostat of animal during acute thermal stress and actively participate in to ameliorate deleterious effect of thermal stress and maintenance of cellular integrity . It is also interesting to see that this gene also show higher expression in winter season as compare to moderate season, the exact reason of this phenomena was unknown but we hypothesized that TNF-α gene is very sensitive to climate change either hot and cold and it may play an important role in the development and maintenance of the homeostatic in animal. However, the exact precise mechanism of action of this gene in this scenario was unknown and thrust to be investigated how this gene was recognized and select the path to either prevent or apoptosis the cells under stress condition. This study may help investigator for future study in the area of stress physiology in animals.   


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