18-Title: Fixation of ‘H’ allele in C/T polymorphism in exon 1 region of growth differentiation factor 9 (GDF9) gene in Indian Muzaffarnagari sheep
Authors: Parul Singh, Deepak Sharma, Satyendra Pal Singh, Madhu Tiwari, Avneesh Kumar, Sanjeev Kumar Singh and Vijay Pandey
Source: Ruminant Science (2020)-9(2):303-304.
How to cite this manuscript: Singh Parul, Sharma Deepak, Singh SP, Tiwari Madhu, Kumar Avneesh, Singh SK and Pandey Vijay (2020). Fixation of ‘H’ allele in C/T polymorphism in exon 1 region of growth differentiation factor 9 (GDF9) gene in Indian Muzaffarnagari sheep. Ruminant Science 9(2):303-304.
In the present study, FecGH (high fertility) polymorphism in exon 1 region of growth differentiation factor 9 (GDF9) gene was investigated using PCR-RFLP method in Muzaffarnagari sheep (n=200). The amplified fragments of the GDF9 showed 139 bp product that was digested with DdeI restriction enzyme. The results revealed only HH genotype (105 and 34 bp; 100%) with ‘H’ allele (1.0). We observed monomorphic pattern in GDF9 gene revealed that FecGH allele seems to be fixed in the screened sheep population. Consequently, we could not perform association analysis with reproduction trait.
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