2-Title: Cytoprotective potential of curcumin and resveratrol during freeze-thaw cycle of buffalo bone marrow derived mesenchymal stem cells
Authors: Navjot Kaur, Manjinder Sharma, Milindmitra K Lonare and Digvijay Singh
Source: Ruminant Science (2020)-9(2):215-222.
How to cite this manuscript: Kaur Navjot, Sharma Manjinder, Lonare MK and Singh Digvijay (2020). Cytoprotective potential of curcumin and resveratrol during freeze-thaw cycle of buffalo bone marrow derived mesenchymal stem cells. Ruminant Science 9(2):215-222.
An endeavour has been made to evaluate the effect of curcumin (CUR) and resveratrol (RES) on the cell viability and apoptosis in buffalo bone marrow derived mesenchymal stem cells (MSCs) during the freeze-thaw cycle. MSCs were isolated and cultured in high glucose DMEM supplemented with 15% FBS. MSCs characterized for alkaline phosphatase (AP) activity and stem cell markers (CD73 and OCT4) showed positive expression. Cells were cryopreserved with CUR @ 3.125 µM and RES @ 0.156 µM alone and in combination (3.125 µM+0.156 µM) in a standardized cryopreservative medium for a period of one month. Standardized cryopreservative medium with 7% DMSO and 3% glycerol in high glucose DMEM served as control. Cell viability was significantly increased (p0.05) with the addition of CUR and RES alone as well as in combination than the control group. Apoptotic index, per cent necrotic cells and per cent dead cells were significantly decreased (p0.05) in bio-antioxidant treated groups. In conclusion, the addition of bio-antioxidants in cryopreservation medium suppressed the apoptosis and improved the post-thaw cell recovery during the freeze-thaw cycle.
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