27-Title: Impact of LEPR/BseGI polymorphism on production and reproduction traits of Haryana cattle
Authors: Rambachan, Rajesh Nigam, Vijay Pandey, Pawanjit Singh, SP Singh and Deepak Sharma
Source: Ruminant Science (2020)-9(2):351-356.
How to cite this manuscript: Rambachan, Nigam Rajesh, Pandey Vijay, Singh Pawanjit, Singh SP and Sharma Deepak (2020). Impact of LEPR/BseGI polymorphism on production and reproduction traits of Haryana cattle. Ruminant Science 9(2):351-356.
This study was aimed to elucidate the genetic polymorphism of the LEPR gene and its correlation with reproduction and production traits in Haryana cattle. For that genomic DNA was harvested from WBCs of 62 Haryana cows. The LEPR/BseGI assay showed two alleles C and T with 0.52 and 0.48 allelic frequency and three genotypes CT, CC, and TT with 87.09, 8.06 and 4.83 per cent genotypic frequency, respectively in the studied Haryana population. The LEPR/BseGI genotypes showed significant association with GP in the second lactation and with LP, TMY, and MY300 in the first lactation in Haryana cattle. In conclusion, LEPR/BseGI assay revealed genetic polymorphism in Indian Haryana cattle showing significant association with reproduction (GP) and milk production (LP, TMY and MY300) traits. This signifies that this SNP in the LEPR gene may be one among many markers for the selection of cattle with better milk yield. Nevertheless, more studies are needed to do in another breed and population of Indian cattle for authenticating the association of LEPR/BseGI assay with other production and reproduction traits.
Al-Janabi HRA, Al-Rawi AJ and Al-Anbari NN (2020). The relationship between leptin receptor gene polymorphisms and some productive and reproductive traits in Holstein primiparous cows. Plant Archives 20(1):3271-3276.
Al-Janabi HRA, Al-Rawi AJ and Al-Anbari NN (2018). Association of leptin receptor gene polymorphism with some productive and reproductive traits in Holstein primiparous cows. Journal of Entomology and Zoology Studies 6(2):1353-1358.
Almeida SEM, Santos LBS, Passos DT, Corbellini AO, Lopes BMT, Kirst C, Terra G, Neves JP, Gonçalve PBD, Moraes JCF and Weimer TA (2008). Genetic polymorphisms at the leptin receptor gene in three beef cattle breeds. Genetics and Molecular Biology 31(3):680-685.
Chen CC, Chang T and Su HY (2004). Characterization of porcine leptin receptor polymorphisms and their association with reproduction and production traits. Animal Biotechnology 15:89-102.
Clempson AM, Pollott GE, Brickell JS, Bourne NE, Munce N and Wathes DC (2011). Evidence that leptin genotype is associated with fertility, growth, and milk production in Holstein cows. Journal of Dairy Science 94:3618-3628.
Chauhan HD, Patel HA and Priyadarshi BH (2012). Feeding and resting behaviour of lactating Kankrej cows under different housing systems. Ruminant Science 1(2):173-176.
Chilliard Y, Delavaud C and Bonnet M (2005). Leptin expression in ruminants: Nutritional and physiological regulations in relation with energy metabolism. Domestic Animal Endocrinology 29:3-22.
Ghanbari-Baghenoey SS, Ansari-Mahyari H, Asadollahpour-Nanaei M, Rostami MA and Edriss (2014). Association of LEPR gene Polymorphism with milk yield and age at first calving in the Iranian Holstein dairy cows. Journal of Livestock Science and Technology 2(2):39-42.
Glantz M, Mansson HL, Stalhammar H and Paulsson M (2011). Effect of polymorphisms in the leptin, leptin receptor, and acyl-coenzyme A: diacylglycerol acyltransferase 1(DGAT1) genes and genetic polymorphism of milk proteins on cheese characteristics. Journal of Dairy Science 94(7):3295-3304.
Glantz M, Mansson HL, Stalhammar H and Paulsson M (2012). Effect of polymorphism in the leptin, leptin receptor and acyl-CoA: diacylglycerol acyl transferase 1 (DGAT1) genes and genetic polymorphism of milk proteins on bovine milk composition. Journal of Dairy Research 79:110-118.
Gorlov I, Sulimova G, Perchun A and Slozhenkina M (2017). Genetic polymorphism of the rorc, bgh, bghr, lep, lepr genes in Russian hornless cattle breed. Engineering for Rural Development Jelgava, 24.-26.05.2017. DOI: 10.22616/ERDev2017.16.N038
Komisarek J (2010). Impact of LEP and LEPR gene polymorphisms on functional traits in Polish Holstein-Friesian cattle. Animal Science Papers and Reports 28:133-141.
Komisarek J and Dorynek Z (2006). The relationship between the T945M single nucleotide polymorphism in the leptin receptor gene (LEPR) and milk production traits in Jersey cows. Animal Science Papers and Reports 24(4):271-277.
Kumawat Manish, Sharma Vishnu, Karnani Monika and Sharma Sanjita (2018). Effect of complete ration in diverse physical forms on nutrient utilization and lactation performance in indigenous Gir cattle of semi-arid India. Ruminant Science 7(2):241-246.
Liefers SC, Veerkamp RW, Te Pas MFW, Delavaud C, Chilliard Y and Van Der Lende T (2004). A missense mutation in the bovine leptin receptor gene is associated with leptin concentrations during late pregnancy. Animal Genetics 35:138-141.
Mackowski M, Szymoniak K, Szydlowski M, Kamyczek M, Eckert R, Rozycki M, and Switonski M (2005). Missense mutations in exon 4 of the porcine LEPR gene encoding extracellular domain and their association with fatness traits. Animal Genetics 36:135-137.
Matteis G, Scata MC, Grandoni F, Petrera F, Abeni F, Catillo G, Napolitano F and Moioli B (2012). Association analyses of single nucleotide polymorphisms in the leptin and leptin receptor genes on milk and morphological traits in Holstein cows. Open Journal of Animal Sciences 2:174-182.
Moravcikova N, Trakovicka A and Kasarda R (2012). Polymorphism within the Intron Region of the Bovine Leptin Gene in Slovak Pinzgau Cattle. Journal of Animal Science and Biotechnology 245(1):112-115.
Pandey V, Nigam R, Rambachan, Singh P, SinghSP and Madan AK (2016). Plasma leptin and biochemical profile around parturition in primiparous Sahiwal cows. Ruminant Science 5(2):227-233.
Pandey V, Nigam R, Singh SP, Sharma D, Tiwari M and Rambachan (2017). Effect of leptin gene polymorphism on reproduction and production traits in Sahiwal cattle. Ruminant Science 6(2):237-242.
Pandey V, Nigam R, Singh SP, Sharma D, Tiwari M and Chaudhary S (2019). Circulating levels of adipokines and hormones in periparturient primiparous Sahiwal cows. Indian Journal of Animal Sciences 89(6): 645-647
Rambachan, Nigam R, Pandey V, Singh P, Singh SP and Sharma D (2017). Genetic polymorphisms of leptin gene in relation with reproduction traits in Hariana cows. Journal of Animal Research 7(3):1-5.
Rambachan, Nigam R, Pandey V, Singh SP, Sharma D, Tiwari M and Chaudhary S. (2019). Variations in circulating adipocytokine and lipid profile during periparturient period in primiparous Haryana cows. Ruminant Science 8(1):31-34.
Sambrook J and Russel DW (2001). Molecular Cloning: A Laboratory Manual. 3rd Edn, Vol. 1, Cold Spring Harbor Laboratory Press, New York, USA, 2344pp.
Schenkel FS, Miller SP, Moore SS, Li C, Fu A, Lobo S, Mandell IB and Wilton JW (2006). Association of SNPs in the leptin and leptin receptor genes with different fat depots in beef cattle. In: Proceedings of the 8th Word Congress on genetics applied to livestock production, Belo Horizonte, Minas Gerais, Brazil. 13-18 August.
Silva LFP, Vandehaar MJ, Weber-Nielsen MS and Smith GW (2002). Evidence for a local effect of leptin in bovine mammary gland. Journal of Dairy Science 85:3277-3286.
Silva RC, Ferraz JB, Meirelles FV, Eler JP, Balieiro JC, Cucco DC, Mattos EC, Rezende FM, and Silva SL (2012). Associations of single nucleotide polymorphisms in the bovine leptin gene and leptin receptor genes with growth and ultrasound carcass traits in Nellore cattle. Genetics and Molecular Research 11:3721-3728.
Tartaglia LA (1997). The leptin receptor. Journal of Biological Chemistry 272:6093-6096.
Trakovicka A, Moravcíkova N and Miluchova N (2011). The t945m single nucleotide polymorphism of the bovine leptin receptor gene in population of Slovak spotted bulls. Agriculturae Conspectus Scientificus 76(3):161-164.
Trakovicka A, Moravcíkova N, Miluchova N and Navratilova A (2013a). Association of t945m polymorphism in the bovine leptin receptor gene with milk production traits in Slovak spotted cattle. Agriculturae Conspectus Scientificus 2(1):1602-1610.
Trakovicka A, Moravcíkova N, Nadasky R and Kasarda R (2013b). Genetic polymorphisms of leptin and leptin receptor genes in relation with production and reproduction traits in cattle. Acta Biochimica Polonica 60(4):783-787.
Trakovicka A, Moravcikova N, Nadasky R and Kasarda R (2015). Impact of SNPs in candidate genes on economically important traits in Pinzgau cattle. Poljoprivreda 21(1):150-154.
Yadav AK and Mukherjee Anupama (2018). Genetic variants in exon 4 region of FABP3 gene in relation to milk production traits in Sahiwal and Karan Fries cattle. Ruminant Science 7(2):189-198.