29-Title: Evaluation of on-farm milk culture system for identification of mastitis pathogens

Authors: S Saleem Shabaz, D Rani Prameela, D Sreenivasulu and K Sujatha

Source: Ruminant Science (2020)-9(2):363-372.

How to cite this manuscript: Shabaz SS, Prameela DR, Sreenivasulu D and Sujatha K (2020). Evaluation of on-farm milk culture system for identification of mastitis pathogens. Ruminant Science 9(2):363-372.


In the present study, a total of 438 milk samples (clinical and subclinical) were subjected to cultural examination by on-farm culture system as well as with the conventional culture method. On farm, culture system revealed that 240 isolates were of Gram positive organisms with an occurrence of 54.79% and only 1.59% (7) was of Gram negative organisms. The conventional cultural method revealed that 86.98% (381) of organisms belonged to Gram positive Staphylococcal species and 2.05% (9) to Gram negative E. coli similarly with in Staphylococcal species the occurrence of coagulase positive and coagulase negative Staphylococcus were of 71.12% and 28.27%, respectively. In vitro antibiotics sensitivity test result of Gram positive Staphylococcal isolates revealed high sensitivity to Amoxycillin (60.37%) followed by ampicillin (56.65%), enrofloxacin (53.77%), ciprofloxacin (46.22%) and penicillin and (43.39%) and resistance to amikacin (92.45%) followed by kanamycin (90.56%), streptomycin (87.73%), tetracycline (79.24%) and gentamicin (73.5%). Similarly, the isolates of Gram negative E. coli isolates revealed sensitivity to enrofloxacin (66.6%) followed by ciprofloxacin (55.55%), amoxicillin (55.55%), gentamicin (44.44%), tetracycline (33.33%) and resistance to kanamycin (77.77%) followed by amikacin (66.66%), streptomycin (66.66%) and ampicillin (66.66%).


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