29-Title: Evaluation of on-farm milk culture system for identification of mastitis pathogens
Authors: S Saleem Shabaz, D Rani Prameela, D Sreenivasulu and K Sujatha
Source: Ruminant Science (2020)-9(2):363-372.
How to cite this manuscript: Shabaz SS, Prameela DR, Sreenivasulu D and Sujatha K (2020). Evaluation of on-farm milk culture system for identification of mastitis pathogens. Ruminant Science 9(2):363-372.
In the present study, a total of 438 milk samples (clinical and subclinical) were subjected to cultural examination by on-farm culture system as well as with the conventional culture method. On farm, culture system revealed that 240 isolates were of Gram positive organisms with an occurrence of 54.79% and only 1.59% (7) was of Gram negative organisms. The conventional cultural method revealed that 86.98% (381) of organisms belonged to Gram positive Staphylococcal species and 2.05% (9) to Gram negative E. coli similarly with in Staphylococcal species the occurrence of coagulase positive and coagulase negative Staphylococcus were of 71.12% and 28.27%, respectively. In vitro antibiotics sensitivity test result of Gram positive Staphylococcal isolates revealed high sensitivity to Amoxycillin (60.37%) followed by ampicillin (56.65%), enrofloxacin (53.77%), ciprofloxacin (46.22%) and penicillin and (43.39%) and resistance to amikacin (92.45%) followed by kanamycin (90.56%), streptomycin (87.73%), tetracycline (79.24%) and gentamicin (73.5%). Similarly, the isolates of Gram negative E. coli isolates revealed sensitivity to enrofloxacin (66.6%) followed by ciprofloxacin (55.55%), amoxicillin (55.55%), gentamicin (44.44%), tetracycline (33.33%) and resistance to kanamycin (77.77%) followed by amikacin (66.66%), streptomycin (66.66%) and ampicillin (66.66%).
Bauer AW, Kirby WMM, Sherris JC and Turck M (1966). Antibiotic susceptibility testing by a standardized single disc method. American Journal of Clinical Pathology 45:493-496.
Bhanot V, Chaudhri SS, Bisla RS and Singh H (2012). Retrospective Study on Prevalence and antibiogram of mastitis in cows and buffaloes of Eastern Haryana. Indian Journal of Animal Research 46(2):160-163.
Bradley AJ (2002). Bovine mastitis an evolving disease. Veterinary Journal 164:116-128.
Das Gunjan, Lalnunpuia C, Sarma K, Behera SK, Dutta TK and Bandyopadhyay Samiran (2015). Prevalence of Staphylococcus aureus associated sub-clinical mastitis in crossbred cows in Mizoram. Ruminant Science 4(2):167-170.
Das PK and Joseph E (2005). Identification and antibiogram of Microbes associated with buffalo mastitis In Jabalpur, Madhya Pradesh, India. Buffalo Bulletin 24(1):3-9.
Davidson I (1961). Observation on pathogenic Staphylococci in dairy herd during a period of six years. Research in Veterinary Science 2:22.
Diwakar, Akriti, Choudhary Sunita, Meena Dhirendra, Bhati Taruna and Kataria AK (2019). Antibiotic sensitivity pattern of some Staphylococcus aureus isolates from milk from goats with clinical mastitis. Ruminant Science 8(1):19-22.
Erskine RI, Wilson RC and Riddell MG Jr (1992). Intramammary administration of gentamicin as treatment for experimentally induced Escherichia coli mastitis in cows. American Journal of Veterinary Research 53:375-381.
Guterbock WM, VanEenennaam AL and Anderson RJ (1993). Efficacy of intramammary antibiotic therapy for treatment of clinical mastitis caused by environmental pathogens. Journal of Dairy Science 76:3437-3444.
Halasa T, Huijps K, Osteras O and Hogeveen H (2007). Economic effects of bovine mastitis and mastitis management: A review. Veterinary Quarterly 29(1):18-31.
Huma Zul I, Sharma Neelesh, Ahmed Touqeer, Kour Savleen and Pathak AK (2020). Alteration in the hemato-biochemical profile of mastitis affected lactating dairy cattle. Ruminant Science 9(1):33-36.
Hussain R, Khan A, Javed MT and Rizvi F (2012). Possible risk factors associated with mastitis in indigenous cattle in Punjab, Pakistan. Pakistan Veterinary Journal 32:605-608.
Idriss SE, Foltys, Tancin V, Kirchnerova K, Tancinova D and Zaujec K (2014). Mastitis pathogens and their resistance against antimicrobial agents in dairy cows in Nitra, Slovakia. Slovak Journal of Animal Science 47:33-38.
Jagadeeswari M, Hemashenpagam N, Dhanabalan R and Mekala K (2013). Prevalence and invitro antibiogram pattern of different antibiotics against major bovine mastitis pathogens. International Journal of Pharmaceutical Research and Bio-Science 2(2):112-121.
Jones GF and Ward GE (1990) Evaluation of systemic administration of gentamicin for treatment of coliform mastitis in cows. Journal of the American Veterinary Medical Association 197:731-735.
Joshi S and Gokhale S (2006). Status of mastitis as an emerging disease in improved and periurban dairy farms in India. Annals of the New York Academy of Sciences 1081:74- 83.
Karthikeyan A (2003). Clinicopathological and ultrasonographic studies in bovine mastitis. MVSc Thesis submitted to Tamilnadu Veterinary and Animal Sciences University, Chennai, India.
Katheria Dharmendra, Gangwar LS, Rashmi and Kumar Amit (2016). Prospects and constraint faced by small holder dairy farmers and animal health service provider in controlling mastitis. Ruminant Science 5(1):51-54.
Keefe. Greg, McCarron Jennifer, MacDonald Kimberley and Cameron Marguerite (2011). Using On-Farm mastitis culturing. WCDS Advances in Dairy Technology 23: 81-91.
Kumar R and Sharma A (2002). Prevalence, etiology and antibiogram of mastitis in cows and buffaloes in Hisar, Haryana. Indian Journal of Animal Science 72:361-363.
Kumar Vaibhav, Patel JS, Patel BR, Mevada VK and Raval AP (2012). Therapeutic efficacy of antimicrobial drugs in clinical mastitis of cross bred cattle. Ruminant Science 1(2):177-180.
Madushanka DNN, Padmakumara HMS, Kumarasinghe GDN, Sanjeewa MPK and Magamage MPS (2017). Effect of two different bedding systems on udder health management of dairy cows. Ruminant Science 6(1):1-6.
Markey BK, Leonard FC, Archambault M, Cullinane A and Maguire D (2013). Clinical Veterinary Microbiology. 2nd Edn, Elsevier, Edinburgh.
Neeser NL, Hueston WD, Godden SM and Bey RF (2006). Evaluation of the use of an on-farm system for bacteriologic culture of milk from cows with low-grade mastitis. Journal of American Veterinary Medical Association 228:254-260.
Oliver SP, Gonzalez RN, Hogan JS, Jayarao BM and Owens WE (2004). Microbiological procedures for the diagnosis of bovine udder infection and determination of milk quality. 4th Edn, National Mastitis Council, Inc, Verona, WI.
Othman N and Bahaman AR (2005). Prevalence of subclinical mastitis and antibiotic resistant bacterial in three selected cattle farms in Serdang, Selangor and Klaung, Johor. Journal of Veterinary-Malaysia 17(1):27-31.
Prabhakar R, Priyadarshini M and Prabhu M (2018). Isolation and antibiotic sensitivity of bovine peracute mastitis in field conditions. (2018). National Symposium on “Innovations in Animal Health – Current Challenges and future prospective” & XXXI Annual Convention of IAVMI, 29- 31. January, 2018, CVSc, Tirupati.
Pyorala S, Kaartinen L, Kack H and Rainio V (1994). Efficacy of two therapy regimens for treatment of experimentally induced Escherichia coli mastitis in cows. Journal of Dairy Science 77:453-461.
Qayyum A, Khan JA, Hussain R, Khan A, Avais M, Ahmad N and Hassan MF (2016). Molecular characterization of Staphylococcus aureus isolates recovered from natural cases of subclinical mastitis in Holstein cattle and their antibacterial susceptibility. Pakistan Journal of Agriculture Science 53(4):2016.
Raguvaran R, Mondal DB, Jithin MV, Kumar Bipin and Sivakumar M (2016). Staphylococcus aureus mastitis in a nondescript goat Ruminant Science 5(2):291-292.
Raorane Abhay, Chothe Shubhadha, Dubal ZB, Barbuddhe SB, Karunakaran M, Doijad Swapnil, Pathak Ajay, Poharkar Krupali and Singh NP (2013). Antimicrobial resistance of the pathogens isolated from bovine mastitis in Goa. Ruminant Science 2(2):139-144.
Roberson Jr, Warnick LD and Moore G (2003). Mild to moderate clinical mastitis: Efficacy of intramammary amoxicillin, frequent milk out, a combined intramammary amoxicillin, and frequent milk out treatment versus no treatment. Journal of Dairy Science 87:583-592.
Ruegg PL, Godden S, Lago A, Bey R and Leslie K (2012). On-Farm culturing for better milk quality–Engormix.
Sahoo SS, Sahoo N and Parida GS (2009). Antibiogram of bacterial isolates from bovine subclinical mastitis. Indian Veterinary Journal 86(12):1298-1299.
Saravanan S and Palanivel KM (2020). Acute gangrenous and haemorrhagic mastitis due to bacillus species in a preparturient cow. Ruminant Science 9(1):199-201.
Savita, Singh AP, Nayak TC, Chahar A, Yadav R and Kachhawa JP (2020). Isolation and identification of bacteria in subclinical mastitis in cattle from Bikaner city. Ruminant Science 9(1):45-48.
Schroeder J (2010). Mastitis Control Programs: Bovine Mastitis and Milking Management. Extension Bulletin as-1129: North Dakota State University.
Santos VM, Martins HB and Rezende IS (2014). Virulence factor profile of Staphylococcus aureus isolated from bovine milk from Brazil. Food Nutrition Science 5:1496-1505.
Singh Pawanjit, Nigam Rajesh, Kumar Amit and Pandey Vijay (2018). Isolation and molecular characterization of pathogens associated with mastitis in Sahiwal cows. Ruminant Science 7(1):43-46.
Spencer GR and Lasmanis J (1952). Reservoirs of Micrococcus pyogenes in bovine mastitis. American Journal of Veterinary Research 13:500.
Sumathi BR, Veeregowda BM and Gomes AR (2008). Prevalence and antibiogram profile of bacterial isolates. Journal of Animal Health 39(2):79- 81.
Sunita, Diwakar and Kataria AK (2017). Antibiotic resistance pattern of Staphylococcus aureus isolated from milk of cattle with clinical mastitis. Ruminant Science 6(2):319-322.
Tufani NA, Makhdoomi DM and Hafiz A (2012). Epidemiology and therapeutic management of bovine mastitis. Indian Journal of Animal Research 46(2):148-151.
Wahid NAA, Al Atabi AC and Saeed AA (2017). Isolation and identification study of E. coli from buffalo mastitis and antimicrobial susceptibility pattern of its. Kufa Journal for Veterinary Medical Sciences 8:2.