30-Title: Effect of phase of oestrus cycle on recovery and in vitro maturation of abbatoir derived buffalo oocytes
Authors: MK Shukla, D Kumar, Ashitha Suresh, Neeraj Verma, OP Shrivastava and BC Sarkhel
Source: Ruminant Science (2020)-9(2):373-377.
How to cite this manuscript: Shukla MK, Kumar D, Suresh Ashitha, Verma Neeraj, Shrivastava OP and Sarkhel BC (2020). Effect of phase of oestrus cycle on recovery and in vitro maturation of abbatoir derived buffalo oocytes. Ruminant Science 9(2):373-377.
Abstract
This study was conducted on oocytes retrieved from 1339 follicles (406 ovaries) in follicular phase, 1191 follicles (371 ovaries) in the early luteal phase and 1121 follicles (233 ovaries) in luteal phase to study the effect of the phase of oestrous cycle on oocyte retrieval, quality and in vitro maturation of the oocytes. Significantly (p<0.01) higher number of follicles per ovary and COCs retrieved per ovary were recorded in the ovaries in luteal phase (4.87±0.32 and 3.29±0.23) as compared to those in follicular phase (3.34±0.12 and 2.28±0.09) or early luteal phase (3.23±0.13 and 2.26±0.11). The in vitro maturation (%) was also significantly (p<0.01) higher in COCs retrieved from ovaries during the luteal phase (86.54±0.53) as compared to follicular (80.86±0.23) or early luteal phase (83.76±0.48) of the oestrus cycle. The difference between nuclear maturation in COCs retrieved during early luteal and follicular phase was also significant (p<0.05). Thus the present study indicates that COCs retrieved during the luteal phase of the cycle have better developmental competence as compared to those removed during other stages of the oestrous cycle.
References
Agrawal Jitendra Kumar, Saxena Atul, Kharche SD, Pathak Juhi, Sikarwar AKS, Patel Akhil, Goel AK and Jindal SK (2016). A study on parthenogenetic activation and embryo production from in vitro matured caprine oocytes. Ruminant Science 5(1):21-24.
Agrawal JK, Kharche SD, Saxena A, Yadav S and Panday RP (2017). A study on embryo production through in vitro fertization of caprine oocytes. Ruminant Science 6(2):333-336.
Baruselli PS, Mucciolo RG, Visintin JA, Viana WG, Arruda RP, Madureira EH, Oliveira CA and Molero-Filho JR (1997). Ovarian follicular dynamics during the estrous cycle in buffalo. Theriogenology 47:1531-1547.
Chian RC, Chung JT, Downey BR and Tan SL (2002). Maturational and developmental competence of immature oocytes retrieved from bovine ovaries at different phases of folliculogenesis. Reproductive Biomedicine Online 4(2):127-132.
Goto K, Takuma Y, Ooe N and Ogawa K (1990). In vitro development of bovine oocytes collected from ovaries of individual cows after in vitro fertilization. Japanese Journal of Animal Reproduction 36(2):110-113.
Hagemann LJ, Beaumont SE, Berg M, Donnison MJ, Ledgard A, Peterson AJ, Schurmann A and Tervit HR (1999). Development during single IVP of bovine oocytes from dissected follicles: Interactive effects of oestrous cycle stage, follicle size and atresia. Molecular Reproduction and Development 53:451-458.
Hunter AG and Moor RM (1987). Stage dependent effects of inhibiting ribonucleic acids protein synthesis on meiotic maturation of bovine oocytes in-vitro. Journal of Dairy Science 70:646-1651.
Jamil H, Samad HA, Qureshi ZA, Rehman NU and Lodhi LA (2008). Harvesting and evaluation of riverine buffalo follicular oocytes. Turkish Journal of Veterinary and Animal Sciences 32(1):25-30.
Kumar D, Gopalakrishna R, Singh AP, Ranjan R, Pandey SK and Sarkhel BC (2014). Developmental potency of pre-implant parthenogenetic goat embryos: Effect of activation protocols and culture media. In vitro Cellular and Developmental Biology-Animal 50(1):1-6.
Kumar Dharmendra, Shukla MK, Jeena LM, Rahangdale S, Kumari D, Singh A and Sarkhel BC (2016). Assessment of in vitro maturation of oocytes derived from slaughterhouse goat ovaries. Ruminant Science 5(1):17-20.
Lakhera A (2015). Harvesting the reproductive potential of culled cows by in vivo and in vitro ovum pick up technique. PhD thesis submitted to NDVSU, Jabalpur.
Machatkova M, Hulinska P, Horakova J, Reckova Z and Hanzalova K (2008). Oestrous cycle stage influences the morphology and maturation of porcine oocytes in vitro. Veterinarni Medicina 53(2):70-76.
Machatkova M, Jokesova E, Horky F and Krepelova A (2000). Utilization of the growth phase of the first follicular wave for bovine oocyte collection improves blastocyst production. Theriogenology 54:543-550.
Mahesh YM, Rao MM, Sudhakar P and Rao KRSS (2014). Effect of harvesting technique and presence or absence of corpus luteum on in vitro development after parthenogenetic activation of oocytes recovered from buffalo ovaries. Veterinary World 7(5):315-320.
Manjunatha BM (2006). Studies on in vitro production and cryopreservation of buffalo embryos. PhD thesis submitted to Karnataka Veterinary Animal and Fishery Science University, Bidar.
Puri Gopal and Bag Sadhan (2014). Generation of parthenogenetic embryos by ethanol activation in buffalo. Ruminant Science 3(1):5-7.
Ratky J, Torner H, Egerszegi I, Schneider F, Sarlos P, Manabe N and Brussow KP (2005). Ovarian activity and oocyte development during follicular development in pigs at different reproductive phases estimated by the repeated endoscopic method. Journal of Reproduction and Development 51:109-115.
Sadeesh EM, Shah Fozia, Balhara AK, Thirumaran SMK, Balhara Sunesh and Yadav PS (2013). Effect of thiol compound supplemented culture medium on in vitro maturation of buffalo oocytes. Ruminant Science 2(1):59-62.
Schnurrbusch U, Schmette C and Elze K (1990). Recovery and light microscopic evaluation of follicular oocytes of swine and relationship between the degeneration rate of oocytes and the estrus phase. Deutsche Tierarztliche Wochenschrift 97:390-395.
Sharma AK, Chaudhary SS and Puri Gopal (2017). In-vitro maturation of buffalo oocytes in TCM-199 media supplemented with follicular fluid, hormones and antioxidants. Ruminant Science 6(1):13-18.
Shukla MK, Shrivastava OP, Kumar D, Shukla SN, Jain Neera and Sarkhel BC (2016). Effect of follicle size on glucose-6-phosphate dehydrogenase activity of buffalo oocytes. Ruminant Science 5(2):243-245.
Singh Vijay, Misra AK, Kumar Suresh and Kumar Vijay (2015a). Effect of seasons on in-vitro maturation and cleavage of oocyte and in-vitro development of buffalo embryo. Ruminant Science 4(1):29-31.
Singh Vijay, Misra AK, Kumar Suresh and Kumar Vijay (2015b). Effect of growth factors on in vitro maturation and in vitro culture of buffalo oocytes. Ruminant Science 4(2):219-224.
Varisanga MD, Sumantri C, Murakami M, Fahrudin M and Suzuki T (1998). Morphological classification of the ovarian in relation to the subsequent oocyte quality for IVF-produced bovine embryos. Theriogenology 50:1015-1023.
Zaraza J, Oropeza MA, Kosawe D, Hermann JW, Carnwath H and Niemann H (2010). Developmental competence and mRNA expression of preimplantation in vitro produced embryos from prepubertal and postpubertal cattle and their relationship with apoptosis after intraovarian administration of IGF-1. Theriogenology 74(1):75-89.