31-Title: Seasonal changes in seminal plasma biochemical parameters of NARI Suwarna rams

Authors: Venkanagouda Doddagoudar, MK Tandle, RG Bijurkar, NA Patil, Ashok Pawar, Shrikant Kulkarni and Vinay Tikare

Source: Ruminant Science (2020)-9(2):379-382.

How to cite this manuscript: Doddagoudar Venkanagouda, Tandle MK, Bijurkar RG, Patil NA, Pawar Ashok, Kulkarni Shrikant and Tikare Vinay (2020). Seasonal changes in seminal plasma biochemical parameters of NARI Suwarna rams. Ruminant Science 9(2):379-382.


The seminal plasma composition of NARI Suwarna rams, a strain of sheep with improved reproductive capability was evaluated over six months, divided equally into breeding (winter) and non-breeding (summer) seasons. The lipid peroxidation during the non-breeding season was significantly higher, whereas all other estimated parameters viz. total protein, cholesterol, AST, ALT and ALP did not show any significant variation (p>0.05) between or within breeding (winter) and non-breeding (summer) seasons.


Ashworth PJC, Harrison RAP, Miller NGA, Plummer JM and Watson PF (1994). Survival of ram spermatozoa at high dilution: Protective effect of simple constituents of culture media as compared with seminal plasma. Reproduction, Fertility and Development 6(2):173-80.

Ayala A, Muñoz MF and Argüelles S (2014). Lipid peroxidation: Production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxidative Medicine and Cellular Longevity 2014:1-31.

Barrios B, Pérez-Pé R, Gallego M, Tato A, Osada J, Muiño-Blanco T and Cebrián-Pérez JA (2000) Seminal plasma proteins revert the cold-shock damage on ram sperm membrane. Biology of Reproduction 63(5):1531-7.

Benmoula A, Badi A, El Fadili M, EL Khalil K, Allai L, El Hilali A and El Amiri B (2017). Effect of season on scrotal circumference, semen characteristics, seminal plasma composition and spermatozoa motility during liquid storage in INRA180 rams. Animal Reproduction Science 180:17-22.

Ciereszko A, Glogowski J, Strzezek J and Demianowicz W (1992). Low stability of aspartate aminotransferase activity in boar semen. Theriogenology 37(6):1269-1281.

De Neergaard R, Nielsen JE, Jorgensen A, Toft BG, Goetze JP and Jorgensen N (2018). Positive association between cholesterol in human seminal plasma and sperm counts: results from a cross-sectional cohort study and immunohistochemical investigations. Andrology 6(6):817-828.

Gundogan M (2006). Some reproductive parameters and seminal plasma constituents in relation to season in Akkaraman and Awassi rams. Turkish Journal of Veterinary and Animal Science 30:95-100.

Kaimal P (2015). Studies on reproductive traits, semen preservation at refrigeration temperature and its evaluation in NARI Suwarna strain of sheep. MVSc thesis submitted to KVAFSU, Bidar, Karnataka.

Kapadiya PS, Nakhashi HC, Chauhan PM, Sutaria TV and Suthar BN (2018). Efficacy of melatonin as an antioxidant for cryopreservation of Kankrej bull semen. Ruminant Science 7(1):89-92.

Mustapha AR, Beigh YA, Abhishek K, Rahul K, Omer D, Kumar A, Prasad JK, Bhure S, Srivastava N  and Ghosh SK (2017). Effects of partial deoxygenation of extender on plasma membrane integrity and enzyme activity in frozen-thawed crossbred bull semen. Ruminant Science 6(2):327-331.

Pandey Vijay, Nigam Rajesh, Saxena Atul, Singh Pawanjit, Sharma Ambika, Swain Dilip Kumar, Sharma Lokesh and Dixit Shilpi (2012). Biochemical attributes of cattle and buffalo bull semen: A comparative study. Ruminant Science 1(1):59-62.

Perumal P, Khate K and Rajkhowa C (2013). Effect of foot and mouth disease vaccination on seminal and biochemical profiles of Mithun (Bos frontalis) semen. Asian Pacific Journal of Reproduction 2(3):178-84.

Rao B, Soufir JC, Martin M and David G (1989). Lipid peroxidation in human spermatozoa as related to midpiece abnormalities and motility. Gamete Research 24(2):127-34.

Sikarwar AKS, Sinha NK, Ramachandran N, Ranjan R, Gangwar Chetna and Agrawal JK (2015). SDS-PAGE profile of seminal plasma proteins of Sirohi buck semen. Ruminant Science 4(2):213-214.

Singh Vipin, Kumar Anuj, Yadava CL, Patel Akhil, Yadav Brijesh, Panday Vijay and Saxena Atul (2018). Effect of season on certain seminal plasma enzymes profile at pre-freeze and post thaw period in the Haryana bull. Ruminant Science 7(2):279-282.

Tejaswi V, Swamy MN, Yathiraj S, Honnappa TG and Isloor S (2016). Enzymatic activities in fresh seminal plasma and extended refrigerated semen in NARI Suvarna rams. Theriogenology Insight 6(1):27.

Tejaswi V, Swamy M Narayana, Isloor Shrikrishna and Shwetha HS (2020). Enzymatic and electrolyte profile in seminal plasma of NARI Suwarna rams. Ruminant Science 9(1):69-70.

Turner RMO and McDonnell SM (2003). Alkaline phosphatase in stallion semen: Characterization and clinical applications. Theriogenology 60(1):1-10.

Yeni D, Gundogan M, Hakki Cigerci I, Avdatek F and Fatih Fidan A (2010). Seasonal variation of oxidative stress parameters in ram seminal plasma. Journal of Animal and Veterinary Advances 9(1):49-54.