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32-Title: Efficacy of epididymal spermatozoa for in vitro fertilization in caprine species - Anandpub

32-Title: Efficacy of epididymal spermatozoa for in vitro fertilization in caprine species

Authors: A Suresh, MK Shukla, Dharmendra Kumar, OP Shrivastava and Neeraj Verma

Source: Ruminant Science (2020)-9(2):383-386.

How to cite this manuscript: Suresh A, Shukla MK, Kumar Dharmendra, Shrivastava OP and Verma Neeraj (2020). Efficacy of epididymal spermatozoa for in vitro fertilization in caprine species. Ruminant Science 9(2):383-386.

Abstract

The fertilization capacity of caprine epididymal spermatozoa in an in vitro embryo production system was evaluated in this study. Oocytes and spermatozoa were recovered from ovaries and epididymis, respectively, procured from a local small animal abattoir. The abattoir derived cumulus oocyte complexes (COCs) were subjected to in vitro maturation and in vitro fertilization with epididymal spermatozoa harvested from the epididymis of slaughtered animals collected within 2-3 hours of slaughter and stored at 4oC up to 24 hours. Cytoplasmic maturation, cleavage and blastocyst rates of 89.78±0.50, 63.88±2.33 and 25.38±2.08%, respectively were recorded for in vitro embryo production with abattoir derived male and female gametes. In conclusion, the use of abattoir derived spermatozoa for in vitro embryo production can be useful for the conservation of elite germplasm from dead animals.

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